Restriction map for
pF1KB4367
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AhdI ApaI AscI Asp718I AspEI
AvaI AvrII BamHI BbeI BciVI
BcnI BglI BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT14I FseI
HapII HpaII KasI KpnI MamI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI StuI
StyI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 44 CGCG CG^CG
AclI 55 AACGTT AA^CGTT
AcyI 3042 GGCGTC G[GA]^CG[CT]C
AflIII 687 ACATGT A^C[GA][CT]GT
Alw44I 2174 GTGCAC G^TGCAC
ApaLI 2174 GTGCAC G^TGCAC
AseI 1621 ATTAAT AT^TAAT
AsnI 1621 ATTAAT AT^TAAT
Asp700I 379 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 693 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 435 GGACC G^G[AT]CC
AviII 2314 TGCGCA TGC^GCA
BanII 1828 GAGCTC G[GA]GC[CT]^C
BbrPI 3028 CACGTG CAC^GTG
BbvCI 2167 GCTGAGG CCTCAGC(-5/-2)
BcgI 336 CGAGTCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1530 AGATCT A^GATCT
BsaAI 3028 CACGTG [CT]AC^GT[GA]
BsaHI 3042 GGCGTC G[GA]^CG[CT]C
BsmFI 965 GTCCC GGGAC(10/14)
BspLU11I 687 ACATGT A^CATGT
BspMI 179 ACCTGC ACCTGC(4/8)
BsrDI 2448 CATTGC GCAATG(2/0)
BstAPI 1792 GCATATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 44 CGCG CG^CG
BstYI 1530 AGATCT [GA]^GATC[CT]
DrdI 400 GACACTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 43 CCGCGG C^C[GA][CT]GG
EcoNI 1939 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 491 ATGCAT ATGCA^T
FauI 42 CCCGC CCCGC(4/6)
FspI 2314 TGCGCA TGC^GCA
Hin1I 3042 GGCGTC G[GA]^CG[CT]C
HpaI 1027 GTTAAC GTT^AAC
KspI 43 CCGCGG CCGC^GG
MflI 1530 AGATCT [GA]^GATC[CT]
MvnI 44 CGCG CG^CG
NdeI 800 CATATG CA^TATG
NheI 2484 GCTAGC G^CTAGC
NsiI 491 ATGCAT ATGCA^T
PflFI 693 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1189 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3028 CACGTG CAC^GTG
PmlI 3028 CACGTG CAC^GTG
PshBI 1621 ATTAAT AT^TAAT
Psp1406I 55 AACGTT AA^CGTT
SacI 1828 GAGCTC GAGCT^C
SacII 43 CCGCGG CCGC^GG
SpeI 2257 ACTAGT A^CTAGT
TaqI 3019 TCGA T^CGA
Tth111I 693 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3019 TCGA T^CGA
Van91I 1189 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1530 AGATCT [GA]^GATC[CT]
XmnI 379 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 774 CTTAAG C^TTAAG
2475 CTTAAG
AocI 418 CCTGAGG CC^T[ACGT]AGG
1159 CCTCAGG
Aor51HI 2481 AGCGCT AGC^GCT
2900 AGCGCT
BalI 1356 TGGCCA TGG^CCA
2444 TGGCCA
BanI 423 GGCACC G^G[CT][GA]CC
3034 GGTGCC
BclI 1395 TGATCA T^GATCA
1977 TGATCA
BfrI 774 CTTAAG C^TTAAG
2475 CTTAAG
BpmI 1730 CTGGAG CTGGAG(16/14)
2864 CTGGAG
BsmBI 941 CGTCTC CGTCTC(1/5)
3044 CGTCTC
Bsp1407I 313 TGTACA T^GTACA
2159 TGTACA
BsrGI 313 TGTACA T^GTACA
2159 TGTACA
BstXI 2435 CCACAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2583 CCAGCCAAATGG
Bsu36I 418 CCTGAGG CC^T[ACGT]AGG
1159 CCTCAGG
DraII 282 GGGGCCT [GA]G^G[ACGT]CC[CT]
3003 AGGGCCT
DraIII 726 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2381 CACCAAGTG
EaeI 1356 TGGCCA [CT]^GGCC[GA]
2444 TGGCCA
Eco47III 2481 AGCGCT AGC^GCT
2900 AGCGCT
Eco81I 418 CCTGAGG CC^T[ACGT]AGG
1159 CCTCAGG
EcoO109I 282 GGGGCCT [GA]G^G[ACGT]CC[CT]
3003 AGGGCCT
EcoRI 779 GAATTC G^AATTC
1060 GAATTC
FbaI 1395 TGATCA T^GATCA
1977 TGATCA
HaeII 2481 AGCGCT [GA]GCGC^[CT]
2900 AGCGCT
HgaI 693 GACGC GACGC(5/10)
3043 GCGTC
HincII 1027 GTTAAC GT[CT]^[GA]AC
2247 GTCAAC
HindII 1027 GTTAAC GT[CT]^[GA]AC
2247 GTCAAC
HindIII 740 AAGCTT A^AGCTT
1560 AAGCTT
MluNI 1356 TGGCCA TGG^CCA
2444 TGGCCA
MscI 1356 TGGCCA TGG^CCA
2444 TGGCCA
NspI 687 ACATGT [GA]CATG^[CT]
2849 ACATGC
PstI 1685 CTGCAG CTGCA^G
2552 CTGCAG
PvuII 149 CAGCTG CAG^CTG
2165 CAGCTG
SapI 1825 GAAGAGC GCTCTTC(1/4)
2182 GAAGAGC
SspBI 313 TGTACA T^GTACA
2159 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BbsI BbvI
BfaI BmyI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI