Restriction map for
pF1KB4274
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NgoMIV NheI NlaIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI Sau96I SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 686 GTAGAC GT^[AC][GT]AC
AlwNI 61 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 438 ATTAAT AT^TAAT
AsnI 438 ATTAAT AT^TAAT
BbvI 60 GCAGC GCAGC(8/12)
BpmI 614 CTCCAG CTGGAG(16/14)
BsaJI 190 CCATGG C^C[ACGT][ACGT]GG
BsmI 1029 GAATGC GAATGC(1/-1)
BspHI 1314 TCATGA T^CATGA
BsrFI 1666 ACCGGC [GA]^CCGG[CT]
BssKI 66 CCTGG ^CC[ACGT]GG
BstNI 66 CCTGG CC^[AT]GG
Cfr10I 1666 ACCGGC [GA]^CCGG[CT]
DsaI 190 CCATGG C^C[GA][CT]GG
EaeI 1668 CGGCCA [CT]^GGCC[GA]
EcoRII 66 CCTGG ^CC[AT]GG
EcoT14I 190 CCATGG C^C[AT][AT]GG
EcoT22I 863 ATGCAT ATGCA^T
Fnu4HI 60 GCAGC GC^[ACGT]GC
HapII 1667 CCGG C^CGG
HpaII 1667 CCGG C^CGG
ItaI 60 GCAGC GC^[ACGT]GC
MseI 439 TTAA T^TAA
MspI 1667 CCGG C^CGG
MvaI 66 CCTGG CC^[AT]GG
NcoI 190 CCATGG C^CATGG
NsiI 863 ATGCAT ATGCA^T
NspI 760 ACATGC [GA]CATG^[CT]
PshBI 438 ATTAAT AT^TAAT
PstI 786 CTGCAG CTGCA^G
RcaI 1314 TCATGA T^CATGA
ScaI 511 AGTACT AGT^ACT
ScrFI 66 CCTGG CC^[ACGT]GG
SfcI 786 CTGCAG C^T[GA][CT]AG
SspI 742 AATATT AAT^ATT
StyI 190 CCATGG C^C[AT][AT]GG
Tru9I 439 TTAA T^TAA
TseI 60 GCAGC G^C[AT]GC
XbaI 283 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AluI 237 AGCT AG^CT
567 AGCT
BfaI 284 CTAG C^TAG
358 CTAG
BsmAI 471 GAGAC GTCTC(1/5)
523 GAGAC
BspMI 214 ACCTGC ACCTGC(4/8)
205 GCAGGT
BsrI 230 ACTGG ACTGG(1/-1)
315 ACTGG
BstF5I 532 CATCC GGATG(2/0)
96 GGATG
EcoNI 691 CCTTACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1706 CCTTTAGCAGG
FokI 532 CATCC GGATG(9/13)
96 GGATG
MaeI 284 CTAG C^TAG
358 CTAG
NdeI 1091 CATATG CA^TATG
1511 CATATG
PleI 84 GACTC GAGTC(4/5)
903 GACTC
SfaNI 299 GCATC GCATC(5/9)
810 GCATC
SmlI 166 CTCAAG C^T[CT][GA]AG
958 CTCAAG
StuI 669 AGGCCT AGG^CCT
1451 AGGCCT
TfiI 804 GATTC G^A[AT]TC
1154 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AfaI AlwI ApoI
Asp700I BbsI BclI BpuAI BseRI
BsiYI BslI Bsp1407I BsrDI BsrGI
DdeI DpnII EarI EcoRI FbaI
HaeIII HinfI HphI Ksp632I MaeIII
MboI MboII MnlI MslI NdeII
NlaIII RsaI SapI Sau3AI SspBI
Tsp45I Tsp509I TspRI XmnI