Restriction map for
pF1KB4270
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BanI BbeI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
ApaI 980 GGGCCC GGGCC^C
Asp700I 450 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 674 GAGCTC G[AT]GC[AT]^C
AvaI 671 CCCGAG C^[CT]CG[GA]G
BamHI 824 GGATCC G^GATCC
BbrPI 929 CACGTG CAC^GTG
BbvCI 852 CCTCAGC CCTCAGC(-5/-2)
BciVI 1208 GGATAC GTATCC(6/5)
BcnI 35 CCGGG CC^[GC]GG
BpmI 1116 CTGGAG CTGGAG(16/14)
BsaAI 929 CACGTG [CT]AC^GT[GA]
BsiHKAI 674 GAGCTC G[AT]GC[AT]^C
BsmFI 37 GGGAC GGGAC(10/14)
BsoBI 671 CCCGAG C^[CT]CG[GA]G
BspHI 878 TCATGA T^CATGA
BspLU11I 380 ACATGT A^CATGT
BspMI 656 ACCTGC ACCTGC(4/8)
BsrDI 125 CATTGC GCAATG(2/0)
BstXI 863 CCAGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 824 GGATCC [GA]^GATC[CT]
DraI 922 TTTAAA TTT^AAA
DraII 980 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1205 GACGGATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 149 CCATGG C^C[GA][CT]GG
EarI 236 CTCTTC CTCTTC(1/4)
EcoNI 852 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 980 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 149 CCATGG C^C[AT][AT]GG
HapII 35 CCGG C^CGG
HincII 18 GTTGAC GT[CT]^[GA]AC
HindII 18 GTTGAC GT[CT]^[GA]AC
HpaII 35 CCGG C^CGG
Ksp632I 236 CTCTTC CTCTTC(1/4)
MflI 824 GGATCC [GA]^GATC[CT]
MspI 35 CCGG C^CGG
NciI 35 CCGGG CC^[GC]GG
NcoI 149 CCATGG C^CATGG
PleI 882 GAGTC GAGTC(4/5)
PmaCI 929 CACGTG CAC^GTG
PmlI 929 CACGTG CAC^GTG
PshAI 115 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 191 CAGCTG CAG^CTG
RcaI 878 TCATGA T^CATGA
SacI 674 GAGCTC GAGCT^C
SspI 82 AATATT AAT^ATT
StyI 149 CCATGG C^C[AT][AT]GG
Tsp45I 1193 GTCAC ^GT[GC]AC
XcmI 681 CCAGTGTGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 824 GGATCC [GA]^GATC[CT]
XmnI 450 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 1102 CCGC CCGC(-3/-1)
1180 CCGC
AcsI 451 AAATTC [GA]^AATT[CT]
1129 AAATTC
AflIII 380 ACATGT A^C[GA][CT]GT
928 ACACGT
AlwI 825 GATCC GGATC(4/5)
824 GGATC
ApoI 451 AAATTC [GA]^AATT[CT]
1129 AAATTC
BanII 674 GAGCTC G[GA]GC[CT]^C
980 GGGCCC
BbsI 4 GTCTTC GAAGAC(2/6)
964 GTCTTC
BfaI 367 CTAG C^TAG
1217 CTAG
BpuAI 4 GTCTTC GAAGAC(2/6)
964 GTCTTC
BsaI 891 GAGACC GGTCTC(1/5)
874 GGTCTC
BsiYI 552 CCAGAGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
852 CCTCAGCCAGG
BslI 552 CCAGAGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
852 CCTCAGCCAGG
BsmAI 891 GAGAC GTCTC(1/5)
875 GTCTC
Cfr13I 980 GGGCC G^G[ACGT]CC
981 GGCCC
HaeIII 861 GGCC GG^CC
981 GGCC
MaeI 367 CTAG C^TAG
1217 CTAG
MaeII 930 ACGT A^CGT
1212 ACGT
MaeIII 418 GTAAC ^GT[ACGT]AC
1193 GTCAC
MslI 72 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
909 CATTGACATG
MspA1I 191 CAGCTG C[AC]G^C[GT]G
1102 CCGCTG
NspI 380 ACATGT [GA]CATG^[CT]
401 ACATGC
PstI 658 CTGCAG CTGCA^G
886 CTGCAG
Sau96I 980 GGGCC G^G[ACGT]CC
981 GGCCC
SmlI 819 CTTGAG C^T[CT][GA]AG
1084 CTCAAG
TfiI 951 GATTC G^A[AT]TC
1098 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HinfI HphI ItaI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI SfaNI SfcI Tru9I TseI
Tsp509I TspRI