Restriction map for
pF1KB4251
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BciVI
BfrI BlnI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NarI NcoI NotI
NruI PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfiI SfoI SgrAI
SnaBI SpeI StyI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 2414 GTCTAC GT^[AC][GT]AC
AlwNI 2761 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2022 GGGCCC GGGCC^C
BanI 2806 GGCACC G^G[CT][GA]CC
BbrPI 2027 CACGTG CAC^GTG
BbsI 2466 GAAGAC GAAGAC(2/6)
BbvCI 1984 GCTGAGG CCTCAGC(-5/-2)
BcgI 764 GCATGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2751 TGATCA T^GATCA
BglII 1073 AGATCT A^GATCT
BlpI 1552 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1552 GCTCAGC GC^T[ACGT]AGC
BpuAI 2466 GAAGAC GAAGAC(2/6)
BsaAI 2027 CACGTG [CT]AC^GT[GA]
BsaBI 2662 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1501 ACCGGA [AT]^CCGG[AT]
BseRI 2591 CTCCTC GAGGAG(10/8)
Bsp1407I 1677 TGTACA T^GTACA
BspHI 2877 TCATGA T^CATGA
BsrGI 1677 TGTACA T^GTACA
BssSI 2374 CTCGTG CACGAG(-5/-1)
BstBI 2102 TTCGAA TT^CGAA
CelII 1552 GCTCAGC GC^T[ACGT]AGC
CfoI 2262 GCGC GCG^C
DraII 537 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 2267 CCGTGG C^C[GA][CT]GG
EcoO109I 537 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 543 CCCGC CCCGC(4/6)
FbaI 2751 TGATCA T^GATCA
HhaI 2262 GCGC GCG^C
HinP1I 2262 GCGC G^CGC
HincII 1864 GTCAAC GT[CT]^[GA]AC
HindII 1864 GTCAAC GT[CT]^[GA]AC
MamI 2662 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1885 GCCGGC GCC^GGC
NdeI 2755 CATATG CA^TATG
NgoMIV 1885 GCCGGC G^CCGGC
NspV 2102 TTCGAA TT^CGAA
PaeR7I 1853 CTCGAG C^TCGAG
PflMI 353 CCACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2027 CACGTG CAC^GTG
PmlI 2027 CACGTG CAC^GTG
PpuMI 537 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1950 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 131 CTGCAG CTGCA^G
RcaI 2877 TCATGA T^CATGA
SacI 2182 GAGCTC GAGCT^C
SbfI 130 CCTGCAGG CCTGCA^GG
ScaI 2569 AGTACT AGT^ACT
SfuI 2102 TTCGAA TT^CGAA
Sse8387I 130 CCTGCAGG CCTGCA^GG
SspBI 1677 TGTACA T^GTACA
SspI 1246 AATATT AAT^ATT
StuI 228 AGGCCT AGG^CCT
Van91I 353 CCACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1538 CCATGAAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1853 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 125 GAGCAC G[AT]GC[AT]^C
2182 GAGCTC
BamHI 3 GGATCC G^GATCC
363 GGATCC
BpmI 590 CTCCAG CTGGAG(16/14)
934 CTGGAG
BsgI 583 CTGCAC GTGCAG(16/14)
2068 GTGCAG
BsiHKAI 125 GAGCAC G[AT]GC[AT]^C
2182 GAGCTC
BspLU11I 1868 ACATGT A^CATGT
2786 ACATGT
BspMI 129 ACCTGC ACCTGC(4/8)
478 GCAGGT
BsrDI 1409 CATTGC GCAATG(2/0)
1840 GCAATG
EcoT22I 1180 ATGCAT ATGCA^T
1807 ATGCAT
HgaI 426 GACGC GACGC(5/10)
451 GCGTC
MspA1I 547 CAGCTG C[AC]G^C[GT]G
2893 CAGCTG
NsiI 1180 ATGCAT ATGCA^T
1807 ATGCAT
PleI 1308 GACTC GAGTC(4/5)
199 GAGTC
PvuII 547 CAGCTG CAG^CTG
2893 CAGCTG
SapI 470 GAAGAGC GCTCTTC(1/4)
1119 GCTCTTC
SmaI 533 CCCGGG CCC^GGG
2318 CCCGGG
SmlI 865 CTTGAG C^T[CT][GA]AG
1853 CTCGAG
XmaCI 533 CCCGGG C^CCGGG
2318 CCCGGG
XmaI 533 CCCGGG C^CCGGG
2318 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
Asp700I AvaI AvaII BalI BanII
BbvI BcnI BfaI BglI BmyI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NheI NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI