Restriction map for
pF1KB4237
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI AscI
Asp700I Asp718I AspEI AvrII BbrPI
BbsI BcgI BciVI BfaI BfrI
BglI BlnI BpuAI BsaAI BseAI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoNI
EcoRI EcoRV EcoT22I HindIII HpaI
KpnI KspI MaeI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 144 CGCG CG^CG
Alw44I 324 GTGCAC G^TGCAC
AlwNI 617 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1078 CCTCAGG CC^T[ACGT]AGG
ApaLI 324 GTGCAC G^TGCAC
AseI 1606 ATTAAT AT^TAAT
AsnI 1606 ATTAAT AT^TAAT
AspI 1912 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1277 TGCGCA TGC^GCA
BamHI 1957 GGATCC G^GATCC
BbvCI 1126 GCTGAGG CCTCAGC(-5/-2)
BclI 1889 TGATCA T^GATCA
BglII 2294 AGATCT A^GATCT
BlpI 1572 GCTCAGC GC^T[ACGT]AGC
BpmI 2317 CTGGAG CTGGAG(16/14)
Bpu1102I 1572 GCTCAGC GC^T[ACGT]AGC
BsaBI 1531 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1289 ACCGGA [AT]^CCGG[AT]
BsgI 1165 CTGCAC GTGCAG(16/14)
BsiEI 234 CGGCCG CG[GA][CT]^CG
BsmI 1323 GAATGC GAATGC(1/-1)
Bsp1407I 2352 TGTACA T^GTACA
BspHI 1332 TCATGA T^CATGA
BspLU11I 956 ACATGT A^CATGT
BsrGI 2352 TGTACA T^GTACA
BssSI 871 CACGAG CACGAG(-5/-1)
BstUI 144 CGCG CG^CG
Bsu36I 1078 CCTCAGG CC^T[ACGT]AGG
CelII 1572 GCTCAGC GC^T[ACGT]AGC
DraIII 2020 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 94 GACTGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 515 CCATGG C^C[GA][CT]GG
EagI 234 CGGCCG C^GGCCG
EclXI 234 CGGCCG C^GGCCG
Eco52I 234 CGGCCG C^GGCCG
Eco81I 1078 CCTCAGG CC^T[ACGT]AGG
FbaI 1889 TGATCA T^GATCA
FseI 1362 GGCCGGCC GGCCGG^CC
FspI 1277 TGCGCA TGC^GCA
HgaI 809 GACGC GACGC(5/10)
HincII 679 GTCAAC GT[CT]^[GA]AC
HindII 679 GTCAAC GT[CT]^[GA]AC
MamI 1531 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1377 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 144 CGCG CG^CG
NaeI 1363 GCCGGC GCC^GGC
NcoI 515 CCATGG C^CATGG
NgoMIV 1363 GCCGGC G^CCGGC
PaeR7I 73 CTCGAG C^TCGAG
PflFI 1912 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1606 ATTAAT AT^TAAT
RcaI 1332 TCATGA T^CATGA
ScaI 2336 AGTACT AGT^ACT
SspBI 2352 TGTACA T^GTACA
Tth111I 1912 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1112 CCAGGCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 73 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 569 ACGTGT A^C[GA][CT]GT
956 ACATGT
BalI 316 TGGCCA TGG^CCA
926 TGGCCA
BbeI 68 GGCGCC GGCGC^C
136 GGCGCC
BsaI 664 GAGACC GGTCTC(1/5)
1286 GAGACC
BstAPI 168 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2147 GCAAGCACTGC
BstEII 45 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
BstPI 45 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
EarI 1692 GAAGAG CTCTTC(1/4)
2371 GAAGAG
EcoO65I 45 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
KasI 68 GGCGCC G^GCGCC
136 GGCGCC
Ksp632I 1692 GAAGAG CTCTTC(1/4)
2371 GAAGAG
MaeII 569 ACGT A^CGT
1989 ACGT
MluNI 316 TGGCCA TGG^CCA
926 TGGCCA
MscI 316 TGGCCA TGG^CCA
926 TGGCCA
NarI 68 GGCGCC GG^CGCC
136 GGCGCC
NdeI 1844 CATATG CA^TATG
2276 CATATG
PflMI 308 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2019 CCACTTTGTGG
SacI 1467 GAGCTC GAGCT^C
1715 GAGCTC
SfoI 68 GGCGCC GGC^GCC
136 GGCGCC
SmaI 270 CCCGGG CCC^GGG
1779 CCCGGG
SmlI 73 CTCGAG C^T[CT][GA]AG
400 CTTGAG
TfiI 1314 GAATC G^A[AT]TC
1420 GAATC
Van91I 308 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2019 CCACTTTGTGG
XmaCI 270 CCCGGG C^CCGGG
1779 CCCGGG
XmaI 270 CCCGGG C^CCGGG
1779 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII