Restriction map for
pF1KB4176
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccII AccIII AciI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstPI BstUI
BstXI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KspI MamI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 1288 GGTACC G^GTACC
AccI 465 GTATAC GT^[AC][GT]AC
AclI 299 AACGTT AA^CGTT
AhdI 1252 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1141 GATCC GGATC(4/5)
Asp700I 1224 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1288 GGTACC G^GTACC
AspEI 1252 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 896 GTGCTC G[AT]GC[AT]^C
BanI 1288 GGTACC G^G[CT][GA]CC
BbvI 554 GCAGC GCAGC(8/12)
BmyI 896 GTGCTC G[AGT]GC[ACT]^C
BpmI 368 CTCCAG CTGGAG(16/14)
BsaAI 217 TACGTG [CT]AC^GT[GA]
BsaJI 935 CCATGG C^C[ACGT][ACGT]GG
BsiHKAI 896 GTGCTC G[AT]GC[AT]^C
BsmI 493 GAATGC GAATGC(1/-1)
Bsp1286I 896 GTGCTC G[AGT]GC[ACT]^C
BspHI 785 TCATGA T^CATGA
BspMI 656 ACCTGC ACCTGC(4/8)
Bst1107I 465 GTATAC GTA^TAC
BstAPI 30 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 437 TTCGAA TT^CGAA
BstYI 1140 AGATCC [GA]^GATC[CT]
BstZ17I 465 GTATAC GTA^TAC
DpnII 1141 GATC ^GATC
DsaI 935 CCATGG C^C[GA][CT]GG
Eam1105I 1252 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1038 GAAGAG CTCTTC(1/4)
EcoNI 1333 CCTGTATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 935 CCATGG C^C[AT][AT]GG
Fnu4HI 554 GCAGC GC^[ACGT]GC
ItaI 554 GCAGC GC^[ACGT]GC
KpnI 1288 GGTACC GGTAC^C
Ksp632I 1038 GAAGAG CTCTTC(1/4)
MboI 1141 GATC ^GATC
MfeI 263 CAATTG C^AATTG
MflI 1140 AGATCC [GA]^GATC[CT]
MslI 213 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1211 CAGCTG C[AC]G^C[GT]G
MunI 263 CAATTG C^AATTG
NcoI 935 CCATGG C^CATGG
NdeII 1141 GATC ^GATC
NheI 352 GCTAGC G^CTAGC
NspI 26 ACATGC [GA]CATG^[CT]
NspV 437 TTCGAA TT^CGAA
PflMI 935 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 299 AACGTT AA^CGTT
PvuII 1211 CAGCTG CAG^CTG
RcaI 785 TCATGA T^CATGA
Sau3AI 1141 GATC ^GATC
SfuI 437 TTCGAA TT^CGAA
StyI 935 CCATGG C^C[AT][AT]GG
TseI 554 GCAGC G^C[AT]GC
Van91I 935 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 68 CCAGGTTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1140 AGATCC [GA]^GATC[CT]
XmnI 1224 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 31 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1103 CAGTGGCTG
AvaII 60 GGACC G^G[AT]CC
316 GGTCC
BbsI 3 GAAGAC GAAGAC(2/6)
202 GTCTTC
BfaI 353 CTAG C^TAG
638 CTAG
BpuAI 3 GAAGAC GAAGAC(2/6)
202 GTCTTC
BsmFI 59 GGGAC GGGAC(10/14)
454 GGGAC
BssKI 68 CCAGG ^CC[ACGT]GG
175 CCAGG
BstNI 68 CCAGG CC^[AT]GG
175 CCAGG
DraII 59 GGGACCC [GA]G^G[ACGT]CC[CT]
315 AGGTCCT
EcoO109I 59 GGGACCC [GA]G^G[ACGT]CC[CT]
315 AGGTCCT
EcoRII 68 CCAGG ^CC[AT]GG
175 CCAGG
HaeIII 232 GGCC GG^CC
664 GGCC
HphI 703 GGTGA GGTGA(8/7)
1010 TCACC
MaeI 353 CTAG C^TAG
638 CTAG
MaeII 218 ACGT A^CGT
300 ACGT
MvaI 68 CCAGG CC^[AT]GG
175 CCAGG
MwoI 30 GCAGTTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1114 GCCAATTATGC
PleI 366 GACTC GAGTC(4/5)
456 GACTC
PpuMI 59 GGGACCC [GA]G^G[AT]CC[CT]
315 AGGTCCT
ScrFI 68 CCAGG CC^[ACGT]GG
175 CCAGG
Tsp45I 220 GTGAC ^GT[GC]AC
580 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsiYI
BslI BsmAI BsrI BstF5I Cac8I
Cfr13I DdeI DraI EcoRI EcoT22I
FokI HinfI MaeIII MboII MnlI
MseI NlaIII NlaIV NsiI RsaI
Sau96I SfaNI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I