Restriction map for
pF1KB4151
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaI BsiEI
BsiWI BsmBI BspDI BspLU11I BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 1765 GTCTAC GT^[AC][GT]AC
AccIII 1148 TCCGGA T^CCGGA
AcsI 2392 GAATTC [GA]^AATT[CT]
AcyI 2410 GGCGTC G[GA]^CG[CT]C
AocI 1297 CCTGAGG CC^T[ACGT]AGG
ApaI 2230 GGGCCC GGGCC^C
ApoI 2392 GAATTC [GA]^AATT[CT]
BbrPI 3088 CACGTG CAC^GTG
BcgI 1935 GCAGTGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3088 CACGTG [CT]AC^GT[GA]
BsaHI 2410 GGCGTC G[GA]^CG[CT]C
BseAI 1148 TCCGGA T^CCGGA
BsmI 1095 GAATGC GAATGC(1/-1)
BspEI 1148 TCCGGA T^CCGGA
BspHI 1226 TCATGA T^CATGA
Bsu36I 1297 CCTGAGG CC^T[ACGT]AGG
DraI 907 TTTAAA TTT^AAA
DraIII 647 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1303 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1297 CCTGAGG CC^T[ACGT]AGG
EcoRI 2392 GAATTC G^AATTC
FseI 1030 GGCCGGCC GGCCGG^CC
HgaI 2411 GCGTC GACGC(5/10)
Hin1I 2410 GGCGTC G[GA]^CG[CT]C
MroI 1148 TCCGGA T^CCGGA
NaeI 1031 GCCGGC GCC^GGC
NcoI 791 CCATGG C^CATGG
NgoMIV 1031 GCCGGC G^CCGGC
NspI 1742 GCATGT [GA]CATG^[CT]
PaeR7I 1100 CTCGAG C^TCGAG
PflMI 477 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3088 CACGTG CAC^GTG
PmlI 3088 CACGTG CAC^GTG
PshAI 2467 GACAGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1226 TCATGA T^CATGA
SapI 591 GCTCTTC GCTCTTC(1/4)
Van91I 477 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2475 TCTAGA T^CTAGA
XhoI 1100 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 31 CGCG CG^CG
572 CGCG
AspI 736 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1842 GACAGAGTC
AvaI 1100 CTCGAG C^[CT]CG[GA]G
1943 CTCGGG
BfaI 1702 CTAG C^TAG
2476 CTAG
BlpI 406 GCTGAGC GC^T[ACGT]AGC
1021 GCTCAGC
Bpu1102I 406 GCTGAGC GC^T[ACGT]AGC
1021 GCTCAGC
BsaWI 1148 TCCGGA [AT]^CCGG[AT]
1772 ACCGGA
BsmAI 742 GTCTC GTCTC(1/5)
1292 GTCTC
BsoBI 1100 CTCGAG C^[CT]CG[GA]G
1943 CTCGGG
Bsp1407I 1745 TGTACA T^GTACA
2726 TGTACA
BsrFI 1031 GCCGGC [GA]^CCGG[CT]
2636 ACCGGC
BsrGI 1745 TGTACA T^GTACA
2726 TGTACA
BstEII 669 GGTTACC G^GT[ACGT]ACC
693 GGTTACC
BstPI 669 GGTTACC G^GT[ACGT]ACC
693 GGTTACC
BstUI 31 CGCG CG^CG
572 CGCG
BstYI 1176 GGATCT [GA]^GATC[CT]
2417 AGATCC
CelII 406 GCTGAGC GC^T[ACGT]AGC
1021 GCTCAGC
Cfr10I 1031 GCCGGC [GA]^CCGG[CT]
2636 ACCGGC
EarI 592 CTCTTC CTCTTC(1/4)
1963 CTCTTC
EcoO65I 669 GGTTACC G^GT[ACGT]ACC
693 GGTTACC
HincII 1987 GTCAAC GT[CT]^[GA]AC
2266 GTCAAC
HindII 1987 GTCAAC GT[CT]^[GA]AC
2266 GTCAAC
Ksp632I 592 CTCTTC CTCTTC(1/4)
1963 CTCTTC
MaeI 1702 CTAG C^TAG
2476 CTAG
MflI 1176 GGATCT [GA]^GATC[CT]
2417 AGATCC
MslI 538 CACAACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1743 CATGTACATG
MvnI 31 CGCG CG^CG
572 CGCG
NdeI 1160 CATATG CA^TATG
2572 CATATG
PflFI 736 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1842 GACAGAGTC
SacI 409 GAGCTC GAGCT^C
2482 GAGCTC
ScaI 2150 AGTACT AGT^ACT
2837 AGTACT
SfiI 235 GGCCTGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1034 GGCCACCTCGGCC
SspBI 1745 TGTACA T^GTACA
2726 TGTACA
Tsp509I 2393 AATT ^AATT
2951 AATT
Tth111I 736 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1842 GACAGAGTC
XhoII 1176 GGATCT [GA]^GATC[CT]
2417 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaII BalI BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XcmI XmnI