##################################
# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoT14I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 394 AACGTT AA^CGTT
AflIII 809 ACATGT A^C[GA][CT]GT
AlwNI 860 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1282 CCTCAGG CC^T[ACGT]AGG
BbvI 170 GCAGC GCAGC(8/12)
BfaI 1109 CTAG C^TAG
BpmI 319 CTGGAG CTGGAG(16/14)
BsaJI 566 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 133 ACCGGA [AT]^CCGG[AT]
BsmFI 162 GGGAC GGGAC(10/14)
BsmI 1102 GCATTC GAATGC(1/-1)
BspLU11I 809 ACATGT A^CATGT
BspMI 438 ACCTGC ACCTGC(4/8)
BssKI 566 CCTGG ^CC[ACGT]GG
BstBI 719 TTCGAA TT^CGAA
BstNI 566 CCTGG CC^[AT]GG
Bsu36I 1282 CCTCAGG CC^T[ACGT]AGG
Cac8I 885 GCGAGC GC[ACGT]^[ACGT]GC
Cfr13I 1018 GGCCC G^G[ACGT]CC
DdeI 1283 CTCAG C^T[ACGT]AG
DraI 993 TTTAAA TTT^AAA
Eco81I 1282 CCTCAGG CC^T[ACGT]AGG
EcoRI 449 GAATTC G^AATTC
EcoRII 566 CCTGG ^CC[AT]GG
EcoRV 483 GATATC GAT^ATC
EcoT22I 1100 ATGCAT ATGCA^T
Fnu4HI 170 GCAGC GC^[ACGT]GC
HaeIII 1018 GGCC GG^CC
HindIII 114 AAGCTT A^AGCTT
ItaI 170 GCAGC GC^[ACGT]GC
MaeI 1109 CTAG C^TAG
MaeII 395 ACGT A^CGT
MvaI 566 CCTGG CC^[AT]GG
NsiI 1100 ATGCAT ATGCA^T
NspV 719 TTCGAA TT^CGAA
PleI 929 GAGTC GAGTC(4/5)
Psp1406I 394 AACGTT AA^CGTT
Sau96I 1018 GGCCC G^G[ACGT]CC
ScrFI 566 CCTGG CC^[ACGT]GG
SfuI 719 TTCGAA TT^CGAA
TaqI 720 TCGA T^CGA
TseI 170 GCAGC G^C[AT]GC
TthHB8I 720 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BbsI 548 GTCTTC GAAGAC(2/6)
931 GTCTTC
BpuAI 548 GTCTTC GAAGAC(2/6)
931 GTCTTC
BsmAI 126 GTCTC GTCTC(1/5)
379 GTCTC
BsrI 318 ACTGG ACTGG(1/-1)
618 ACTGG
BstF5I 280 GGATG GGATG(2/0)
938 GGATG
BstYI 647 AGATCC [GA]^GATC[CT]
1218 AGATCC
FokI 280 GGATG GGATG(9/13)
938 GGATG
HapII 134 CCGG C^CGG
882 CCGG
HpaII 134 CCGG C^CGG
882 CCGG
MflI 647 AGATCC [GA]^GATC[CT]
1218 AGATCC
MspI 134 CCGG C^CGG
882 CCGG
NspI 29 ACATGC [GA]CATG^[CT]
809 ACATGT
SspI 654 AATATT AAT^ATT
715 AATATT
XhoII 647 AGATCC [GA]^GATC[CT]
1218 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
DpnII HinfI HphI MaeIII MboI
MboII MnlI MseI MslI NdeII
NlaIII RsaI Sau3AI SfcI TfiI
Tru9I Tsp45I Tsp509I TspRI