Restriction map for
pF1KB4115
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccII AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII BamHI BbeI BbrPI BbvCI
BfrI BlpI BpmI Bpu1102I BsaAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI FspI HaeII HpaI
KasI KpnI KspI MluI MvnI
NarI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI SwaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1154 GTCGAC GT^[AC][GT]AC
AccIII 1841 TCCGGA T^CCGGA
AclI 253 AACGTT AA^CGTT
AcyI 1469 GGCGTC G[GA]^CG[CT]C
AflIII 1866 ACACGT A^C[GA][CT]GT
AspI 1834 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1971 CCTAGG C^CTAGG
BanI 2320 GGTGCC G^G[CT][GA]CC
BbsI 532 GAAGAC GAAGAC(2/6)
BcgI 795 GCACCAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2527 GGATAC GTATCC(6/5)
BclI 609 TGATCA T^GATCA
BglI 112 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1971 CCTAGG C^CTAGG
BpuAI 532 GAAGAC GAAGAC(2/6)
BsaHI 1469 GGCGTC G[GA]^CG[CT]C
BsaI 726 GGTCTC GGTCTC(1/5)
BsaWI 1841 TCCGGA [AT]^CCGG[AT]
BseAI 1841 TCCGGA T^CCGGA
BspEI 1841 TCCGGA T^CCGGA
BsrFI 2606 GCCGGC [GA]^CCGG[CT]
BssSI 1727 CACGAG CACGAG(-5/-1)
BstEII 1831 GGTGACC G^GT[ACGT]ACC
BstPI 1831 GGTGACC G^GT[ACGT]ACC
BstXI 2517 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2600 GCGC GCG^C
Cfr10I 2606 GCCGGC [GA]^CCGG[CT]
DraII 1112 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1112 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1831 GGTGACC G^GT[ACGT]ACC
EcoRI 2211 GAATTC G^AATTC
FbaI 609 TGATCA T^GATCA
HgaI 1470 GCGTC GACGC(5/10)
HhaI 2600 GCGC GCG^C
Hin1I 1469 GGCGTC G[GA]^CG[CT]C
HinP1I 2600 GCGC G^CGC
MroI 1841 TCCGGA T^CCGGA
MspA1I 2756 CAGCTG C[AC]G^C[GT]G
NaeI 2606 GCCGGC GCC^GGC
NgoMIV 2606 GCCGGC G^CCGGC
NheI 1539 GCTAGC G^CTAGC
PflFI 1834 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 253 AACGTT AA^CGTT
PstI 911 CTGCAG CTGCA^G
PvuII 2756 CAGCTG CAG^CTG
SalI 1154 GTCGAC G^TCGAC
SfaNI 2285 GCATC GCATC(5/9)
SpeI 2194 ACTAGT A^CTAGT
Tth111I 1834 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 540 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1480 GAATTT [GA]^AATT[CT]
2211 GAATTC
ApoI 1480 GAATTT [GA]^AATT[CT]
2211 GAATTC
BglII 699 AGATCT A^GATCT
1887 AGATCT
BsmI 1902 GAATGC GAATGC(1/-1)
352 GCATTC
BsrBI 1772 GAGCGG CCGCTC(-3/-3)
1984 GAGCGG
BsrDI 1894 GCAATG GCAATG(2/0)
2092 GCAATG
FauI 195 GCGGG CCCGC(4/6)
1774 GCGGG
HincII 1154 GTCGAC GT[CT]^[GA]AC
2359 GTCAAC
HindII 1154 GTCGAC GT[CT]^[GA]AC
2359 GTCAAC
HindIII 473 AAGCTT A^AGCTT
2663 AAGCTT
MfeI 566 CAATTG C^AATTG
980 CAATTG
MunI 566 CAATTG C^AATTG
980 CAATTG
NcoI 1584 CCATGG C^CATGG
2517 CCATGG
SexAI 966 ACCAGGT A^CC[AT]GGT
1835 ACCTGGT
SfcI 911 CTGCAG C^T[GA][CT]AG
1977 CTACAG
StuI 1968 AGGCCT AGG^CCT
2048 AGGCCT
XcmI 105 CCACAGTGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2063 CCACAGGAGCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BsaBI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DrdI
DsaI EaeI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI SapI Sau3AI Sau96I
ScrFI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII