Restriction map for
pF1KB4085
##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AviII AvrII BbeI BbsI
BbvCI BcgI BfrI BglI BglII
BlnI BpuAI BseAI BsiWI BsmBI
BspEI BspHI BspLU11I Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
HindIII KasI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SnaBI SpeI Sse8387I SspI StuI
SwaI XcmI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 199 GTCTAC GT^[AC][GT]AC
AocI 37 CCTCAGG CC^T[ACGT]AGG
Asp700I 673 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2584 TGGCCA TGG^CCA
BclI 2574 TGATCA T^GATCA
BlpI 2264 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2264 GCTCAGC GC^T[ACGT]AGC
BsaBI 732 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2830 GAGACC GGTCTC(1/5)
BsaWI 2704 TCCGGT [AT]^CCGG[AT]
BsgI 399 GTGCAG GTGCAG(16/14)
BsmI 2546 GCATTC GAATGC(1/-1)
BspDI 739 ATCGAT AT^CGAT
BssSI 1669 CACGAG CACGAG(-5/-1)
Bsu36I 37 CCTCAGG CC^T[ACGT]AGG
CelII 2264 GCTCAGC GC^T[ACGT]AGC
ClaI 739 ATCGAT AT^CGAT
DraIII 1861 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1027 GAAGAG CTCTTC(1/4)
Eco81I 37 CCTCAGG CC^T[ACGT]AGG
EcoRI 354 GAATTC G^AATTC
EcoRV 577 GATATC GAT^ATC
EcoT14I 1082 CCATGG C^C[AT][AT]GG
FbaI 2574 TGATCA T^GATCA
HpaI 1054 GTTAAC GTT^AAC
Ksp632I 1027 GAAGAG CTCTTC(1/4)
KspI 1840 CCGCGG CCGC^GG
MamI 732 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2584 TGGCCA TGG^CCA
MscI 2584 TGGCCA TGG^CCA
NcoI 1082 CCATGG C^CATGG
NspI 2513 GCATGC [GA]CATG^[CT]
PaeR7I 2432 CTCGAG C^TCGAG
PflMI 1860 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 805 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1840 CCGCGG CCGC^GG
SmaI 120 CCCGGG CCC^GGG
SmlI 2432 CTCGAG C^T[CT][GA]AG
SphI 2513 GCATGC GCATG^C
StyI 1082 CCATGG C^C[AT][AT]GG
Van91I 1860 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 555 TCTAGA T^CTAGA
XhoI 2432 CTCGAG C^TCGAG
XmaCI 120 CCCGGG C^CCGGG
XmaI 120 CCCGGG C^CCGGG
XmnI 673 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 2073 GACGTC GACGT^C
2127 GACGTC
AflIII 1616 ACGTGT A^C[GA][CT]GT
2186 ACGTGT
Alw44I 1624 GTGCAC G^TGCAC
2527 GTGCAC
ApaLI 1624 GTGCAC G^TGCAC
2527 GTGCAC
AspI 1129 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2127 GACGTCGTC
BamHI 124 GGATCC G^GATCC
2157 GGATCC
BbrPI 1861 CACGTG CAC^GTG
1963 CACGTG
BciVI 404 GTATCC GTATCC(6/5)
2593 GTATCC
BsmAI 2830 GAGAC GTCTC(1/5)
2430 GTCTC
Bsp1407I 1973 TGTACA T^GTACA
2246 TGTACA
BsrDI 1200 CATTGC GCAATG(2/0)
94 GCAATG
BsrGI 1973 TGTACA T^GTACA
2246 TGTACA
DraI 2445 TTTAAA TTT^AAA
2674 TTTAAA
HincII 1054 GTTAAC GT[CT]^[GA]AC
2653 GTCAAC
HindII 1054 GTTAAC GT[CT]^[GA]AC
2653 GTCAAC
NaeI 1714 GCCGGC GCC^GGC
1782 GCCGGC
NgoMIV 1714 GCCGGC G^CCGGC
1782 GCCGGC
PflFI 1129 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2127 GACGTCGTC
PmaCI 1861 CACGTG CAC^GTG
1963 CACGTG
PmlI 1861 CACGTG CAC^GTG
1963 CACGTG
PpuMI 245 AGGACCT [GA]G^G[AT]CC[CT]
311 GGGACCT
PvuII 2425 CAGCTG CAG^CTG
2664 CAGCTG
SgrAI 485 CGCCGGTG C[GA]^CCGG[CT]G
1713 CGCCGGCG
SspBI 1973 TGTACA T^GTACA
2246 TGTACA
Tth111I 1129 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2127 GACGTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII