Restriction map for
pF1KB4076
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 1496 TCCGGA T^CCGGA
AflIII 1048 ACATGT A^C[GA][CT]GT
AlwI 186 GATCC GGATC(4/5)
AlwNI 260 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1656 AGCGCT AGC^GCT
AspHI 1164 GAGCAC G[AT]GC[AT]^C
AvaII 26 GGTCC G^G[AT]CC
BanII 211 GAGCCC G[GA]GC[CT]^C
BbsI 518 GTCTTC GAAGAC(2/6)
BbvI 87 GCTGC GCAGC(8/12)
BcnI 29 CCCGG CC^[GC]GG
BglII 1249 AGATCT A^GATCT
BpuAI 518 GTCTTC GAAGAC(2/6)
BseAI 1496 TCCGGA T^CCGGA
BseRI 76 GAGGAG GAGGAG(10/8)
BsiHKAI 1164 GAGCAC G[AT]GC[AT]^C
BspEI 1496 TCCGGA T^CCGGA
BspHI 1389 TCATGA T^CATGA
BspLU11I 1048 ACATGT A^CATGT
BsrDI 1220 GCAATG GCAATG(2/0)
BstEII 39 GGTGACC G^GT[ACGT]ACC
BstPI 39 GGTGACC G^GT[ACGT]ACC
BstYI 1249 AGATCT [GA]^GATC[CT]
Cac8I 824 GCTGGC GC[ACGT]^[ACGT]GC
DraII 1207 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1656 AGCGCT AGC^GCT
EcoO109I 1207 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 39 GGTGACC G^GT[ACGT]ACC
EcoT14I 155 CCTTGG C^C[AT][AT]GG
Fnu4HI 87 GCTGC GC^[ACGT]GC
HaeIII 1208 GGCC GG^CC
ItaI 87 GCTGC GC^[ACGT]GC
MaeII 561 ACGT A^CGT
MflI 1249 AGATCT [GA]^GATC[CT]
MroI 1496 TCCGGA T^CCGGA
MwoI 168 GCTTACTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 29 CCCGG CC^[GC]GG
RcaI 1389 TCATGA T^CATGA
SapI 208 GAAGAGC GCTCTTC(1/4)
SexAI 146 ACCTGGT A^CC[AT]GGT
StyI 155 CCTTGG C^C[AT][AT]GG
TaqI 1329 TCGA T^CGA
TfiI 256 GATTC G^A[AT]TC
TseI 87 GCTGC G^C[AT]GC
TthHB8I 1329 TCGA T^CGA
XhoII 1249 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 1149 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1485 GAAAGCCTTC
BciVI 1442 GGATAC GTATCC(6/5)
1684 GGATAC
BlpI 100 GCTCAGC GC^T[ACGT]AGC
529 GCTCAGC
BmyI 211 GAGCCC G[AGT]GC[ACT]^C
1164 GAGCAC
Bpu1102I 100 GCTCAGC GC^T[ACGT]AGC
529 GCTCAGC
BsaWI 191 TCCGGT [AT]^CCGG[AT]
1496 TCCGGA
BsmFI 1651 GGGAC GGGAC(10/14)
27 GTCCC
Bsp1286I 211 GAGCCC G[AGT]GC[ACT]^C
1164 GAGCAC
Bsp1407I 113 TGTACA T^GTACA
1043 TGTACA
BsrGI 113 TGTACA T^GTACA
1043 TGTACA
CelII 100 GCTCAGC GC^T[ACGT]AGC
529 GCTCAGC
CfoI 828 GCGC GCG^C
1657 GCGC
Cfr13I 26 GGTCC G^G[ACGT]CC
1208 GGCCC
DraI 307 TTTAAA TTT^AAA
582 TTTAAA
EarI 208 GAAGAG CTCTTC(1/4)
1540 GAAGAG
EcoRI 1274 GAATTC G^AATTC
1526 GAATTC
HaeII 827 GGCGCT [GA]GCGC^[CT]
1656 AGCGCT
HhaI 828 GCGC GCG^C
1657 GCGC
HinP1I 828 GCGC G^CGC
1657 GCGC
Ksp632I 208 GAAGAG CTCTTC(1/4)
1540 GAAGAG
MspA1I 822 CAGCTG C[AC]G^C[GT]G
990 CAGCTG
NdeI 1379 CATATG CA^TATG
1463 CATATG
NlaIV 26 GGTCCC GG[ACGT]^[ACGT]CC
954 GGAACC
NspI 1048 ACATGT [GA]CATG^[CT]
1636 ACATGC
PleI 565 GACTC GAGTC(4/5)
485 GAGTC
PvuII 822 CAGCTG CAG^CTG
990 CAGCTG
Sau96I 26 GGTCC G^G[ACGT]CC
1208 GGCCC
ScaI 468 AGTACT AGT^ACT
537 AGTACT
SspBI 113 TGTACA T^GTACA
1043 TGTACA
XmnI 1149 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1485 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BpmI BsaI BsaJI BsiYI BslI
BsmAI BsmI BsrI BssKI BstF5I
BstNI DdeI DpnII EcoRII FokI
HapII HinfI HpaII HphI MaeI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI Tru9I
Tsp45I Tsp509I TspRI