Restriction map for
pF1KB4041
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
AlwNI AocI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BbvI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I Fnu4HI FokI
FseI FspI HgaI Hin1I HindIII
HpaI ItaI KasI KpnI KspI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TseI Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AciI 1331 GCGG CCGC(-3/-1)
AgeI 964 ACCGGT A^CCGGT
Alw44I 1068 GTGCAC G^TGCAC
Aor51HI 278 AGCGCT AGC^GCT
ApaLI 1068 GTGCAC G^TGCAC
AvaI 926 CTCGAG C^[CT]CG[GA]G
AvaII 84 GGACC G^G[AT]CC
BamHI 1376 GGATCC G^GATCC
BanI 346 GGCACC G^G[CT][GA]CC
BbsI 747 GAAGAC GAAGAC(2/6)
BciVI 1040 GGATAC GTATCC(6/5)
BclI 308 TGATCA T^GATCA
BcnI 1217 CCGGG CC^[GC]GG
BpuAI 747 GAAGAC GAAGAC(2/6)
BsaBI 306 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 126 GGTCTC GGTCTC(1/5)
BsaWI 964 ACCGGT [AT]^CCGG[AT]
BseRI 52 GAGGAG GAGGAG(10/8)
BsmI 730 GAATGC GAATGC(1/-1)
BsoBI 926 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1237 TGTACA T^GTACA
BspHI 186 TCATGA T^CATGA
BspMI 330 ACCTGC ACCTGC(4/8)
BsrFI 964 ACCGGT [GA]^CCGG[CT]
BsrGI 1237 TGTACA T^GTACA
BstNI 6 CCAGG CC^[AT]GG
CfoI 279 GCGC GCG^C
Cfr10I 964 ACCGGT [GA]^CCGG[CT]
DraII 83 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1251 GAAGAG CTCTTC(1/4)
Eco47III 278 AGCGCT AGC^GCT
EcoO109I 83 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 6 CCAGG ^CC[AT]GG
EcoT14I 234 CCTTGG C^C[AT][AT]GG
FauI 1331 GCGGG CCCGC(4/6)
FbaI 308 TGATCA T^GATCA
HaeII 278 AGCGCT [GA]GCGC^[CT]
HhaI 279 GCGC GCG^C
HinP1I 279 GCGC G^CGC
HincII 440 GTCAAC GT[CT]^[GA]AC
HindII 440 GTCAAC GT[CT]^[GA]AC
Ksp632I 1251 GAAGAG CTCTTC(1/4)
MamI 306 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1092 CAATTG C^AATTG
MseI 1028 TTAA T^TAA
MunI 1092 CAATTG C^AATTG
MvaI 6 CCAGG CC^[AT]GG
NciI 1217 CCGGG CC^[GC]GG
NdeI 149 CATATG CA^TATG
NspI 1339 GCATGT [GA]CATG^[CT]
PaeR7I 926 CTCGAG C^TCGAG
PflMI 6 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 964 ACCGGT A^CCGGT
PpuMI 83 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 186 TCATGA T^CATGA
SacI 685 GAGCTC GAGCT^C
SapI 1251 GAAGAGC GCTCTTC(1/4)
SgrAI 963 CACCGGTG C[GA]^CCGG[CT]G
SspBI 1237 TGTACA T^GTACA
StyI 234 CCTTGG C^C[AT][AT]GG
Tru9I 1028 TTAA T^TAA
Van91I 6 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 926 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 685 GAGCTC G[AT]GC[AT]^C
1068 GTGCAC
BanII 594 GGGCTC G[GA]GC[CT]^C
685 GAGCTC
BfaI 338 CTAG C^TAG
356 CTAG
BpmI 282 CTCCAG CTGGAG(16/14)
106 CTGGAG
BsiHKAI 685 GAGCTC G[AT]GC[AT]^C
1068 GTGCAC
BsmAI 369 GAGAC GTCTC(1/5)
127 GTCTC
BsmFI 83 GGGAC GGGAC(10/14)
95 GGGAC
BssKI 6 CCAGG ^CC[ACGT]GG
1217 CCGGG
BstYI 956 AGATCC [GA]^GATC[CT]
1376 GGATCC
EaeI 237 TGGCCG [CT]^GGCC[GA]
1266 CGGCCA
HapII 965 CCGG C^CGG
1217 CCGG
HpaII 965 CCGG C^CGG
1217 CCGG
MaeI 338 CTAG C^TAG
356 CTAG
MflI 956 AGATCC [GA]^GATC[CT]
1376 GGATCC
MspI 965 CCGG C^CGG
1217 CCGG
MwoI 25 GCCGACGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
687 GCTCACTCAGC
PleI 142 GAGTC GAGTC(4/5)
929 GAGTC
ScrFI 6 CCAGG CC^[ACGT]GG
1217 CCGGG
SfaNI 102 GATGC GCATC(5/9)
948 GCATC
SfcI 114 CTACAG C^T[GA][CT]AG
170 CTACAG
SmlI 597 CTCAAG C^T[CT][GA]AG
926 CTCGAG
Tsp45I 16 GTGAC ^GT[GC]AC
1262 GTCAC
XhoII 956 AGATCC [GA]^GATC[CT]
1376 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssSI Cfr13I DdeI
DpnII EcoRI HaeIII HinfI HphI
MaeII MaeIII MboI MboII MnlI
MslI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I StuI TaqI TfiI
Tsp509I TspRI TthHB8I XmnI