Restriction map for
pF1KB4035
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI Alw44I AocI ApaI
ApaLI AscI AseI AsnI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BlnI BmyI BpmI BpuAI
BsaAI BsaHI BseAI BsiHKAI BsmI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 958 GGTACC G^GTACC
AccI 820 GTCGAC GT^[AC][GT]AC
AccII 1235 CGCG CG^CG
AhdI 607 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1311 GGATC GGATC(4/5)
Aor51HI 1018 AGCGCT AGC^GCT
Asp700I 1320 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 958 GGTACC G^GTACC
AspEI 607 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 275 GCCTTAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 102 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC
BsaJI 1029 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 1316 ACCGGA [AT]^CCGG[AT]
BsiEI 1302 CGACCG CG[GA][CT]^CG
BsiWI 1289 CGTACG C^GTACG
BsmBI 256 GAGACG CGTCTC(1/5)
BsmFI 1376 GTCCC GGGAC(10/14)
Bsp1407I 467 TGTACA T^GTACA
BsrGI 467 TGTACA T^GTACA
BssSI 1240 CACGAG CACGAG(-5/-1)
BstUI 1235 CGCG CG^CG
CelII 102 GCTGAGC GC^T[ACGT]AGC
Cfr13I 273 GGGCC G^G[ACGT]CC
DraII 272 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 607 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1022 CTCTTC CTCTTC(1/4)
Eco47III 1018 AGCGCT AGC^GCT
EcoO109I 272 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1324 GAATTC G^AATTC
EcoRV 929 GATATC GAT^ATC
EcoT14I 1029 CCTTGG C^C[AT][AT]GG
FauI 1246 GCGGG CCCGC(4/6)
HaeII 1018 AGCGCT [GA]GCGC^[CT]
HaeIII 274 GGCC GG^CC
KpnI 958 GGTACC GGTAC^C
Ksp632I 1022 CTCTTC CTCTTC(1/4)
MspA1I 1275 CAGCTG C[AC]G^C[GT]G
MvnI 1235 CGCG CG^CG
NdeI 953 CATATG CA^TATG
PstI 529 CTGCAG CTGCA^G
PvuII 1275 CAGCTG CAG^CTG
SalI 820 GTCGAC G^TCGAC
SapI 1021 GCTCTTC GCTCTTC(1/4)
Sau96I 273 GGGCC G^G[ACGT]CC
ScaI 965 AGTACT AGT^ACT
SphI 1391 GCATGC GCATG^C
SspBI 467 TGTACA T^GTACA
StyI 1029 CCTTGG C^C[AT][AT]GG
XmnI 1320 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BanI 625 GGCACC G^G[CT][GA]CC
958 GGTACC
BbvI 785 GCAGC GCAGC(8/12)
1274 GCAGC
BclI 134 TGATCA T^GATCA
380 TGATCA
BfaI 1109 CTAG C^TAG
1193 CTAG
BglII 759 AGATCT A^GATCT
1252 AGATCT
BsaBI 367 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
929 GATATCCATC
BseRI 719 CTCCTC GAGGAG(10/8)
1187 CTCCTC
BspMI 824 ACCTGC ACCTGC(4/8)
577 GCAGGT
BstYI 759 AGATCT [GA]^GATC[CT]
1252 AGATCT
FbaI 134 TGATCA T^GATCA
380 TGATCA
HincII 820 GTCGAC GT[CT]^[GA]AC
1261 GTTGAC
HindII 820 GTCGAC GT[CT]^[GA]AC
1261 GTTGAC
MaeI 1109 CTAG C^TAG
1193 CTAG
MamI 367 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
929 GATATCCATC
MflI 759 AGATCT [GA]^GATC[CT]
1252 AGATCT
MslI 177 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
438 CATCCCAGTG
NlaIV 625 GGCACC GG[ACGT]^[ACGT]CC
958 GGTACC
NspI 1070 ACATGC [GA]CATG^[CT]
1391 GCATGC
PleI 1343 GACTC GAGTC(4/5)
334 GAGTC
SexAI 666 ACCAGGT A^CC[AT]GGT
684 ACCTGGT
SfcI 529 CTGCAG C^T[GA][CT]AG
1278 CTGTAG
SmlI 199 CTCAAG C^T[CT][GA]AG
1269 CTTGAG
TseI 785 GCAGC G^C[AT]GC
1274 GCAGC
XcmI 173 CCAGCACCGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1329 CCACTCCCCCATTGG
XhoII 759 AGATCT [GA]^GATC[CT]
1252 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BsaI BsgI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I CfoI DdeI DpnII EcoRII
Fnu4HI FokI HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I