Restriction map for
pF1KB3946
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI AlwI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrDI BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 841 GACGTC GACGT^C
AccI 1504 GTCTAC GT^[AC][GT]AC
Alw44I 661 GTGCAC G^TGCAC
AlwNI 886 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 696 GGGCCC GGGCC^C
ApaLI 661 GTGCAC G^TGCAC
BbrPI 997 CACGTG CAC^GTG
BcgI 359 GCAGGAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 997 CACGTG [CT]AC^GT[GA]
BsaI 859 GAGACC GGTCTC(1/5)
Bsp1407I 1427 TGTACA T^GTACA
BspHI 20 TCATGA T^CATGA
BspMI 890 ACCTGC ACCTGC(4/8)
BsrGI 1427 TGTACA T^GTACA
BssHII 355 GCGCGC G^CGCGC
BstEII 936 GGTCACC G^GT[ACGT]ACC
BstPI 936 GGTCACC G^GT[ACGT]ACC
EcoO65I 936 GGTCACC G^GT[ACGT]ACC
EcoT14I 161 CCAAGG C^C[AT][AT]GG
MaeIII 937 GTCAC ^GT[ACGT]AC
PmaCI 997 CACGTG CAC^GTG
PmlI 997 CACGTG CAC^GTG
PpuMI 1454 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 619 CTGCAG CTGCA^G
PvuII 1201 CAGCTG CAG^CTG
RcaI 20 TCATGA T^CATGA
SacI 400 GAGCTC GAGCT^C
SfiI 1053 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 909 CCCGGG CCC^GGG
SspBI 1427 TGTACA T^GTACA
StyI 161 CCAAGG C^C[AT][AT]GG
TfiI 3 GAATC G^A[AT]TC
Tsp45I 937 GTCAC ^GT[GC]AC
TspRI 1489 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 909 CCCGGG C^CCGGG
XmaI 909 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 152 ACACGT A^C[GA][CT]GT
998 ACGTGT
Asp700I 150 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
327 GAACGCCTTC
AvaI 681 CTCGGG C^[CT]CG[GA]G
909 CCCGGG
BsgI 487 GTGCAG GTGCAG(16/14)
733 GTGCAG
BsmAI 429 GAGAC GTCTC(1/5)
859 GAGAC
BsmFI 1173 GTCCC GGGAC(10/14)
1456 GTCCC
BsoBI 681 CTCGGG C^[CT]CG[GA]G
909 CCCGGG
DpnII 175 GATC ^GATC
1251 GATC
HincII 283 GTCAAC GT[CT]^[GA]AC
871 GTCAAC
HindII 283 GTCAAC GT[CT]^[GA]AC
871 GTCAAC
MboI 175 GATC ^GATC
1251 GATC
NdeII 175 GATC ^GATC
1251 GATC
PleI 85 GACTC GAGTC(4/5)
628 GACTC
Sau3AI 175 GATC ^GATC
1251 GATC
SfaNI 215 GCATC GCATC(5/9)
959 GCATC
XmnI 150 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
327 GAACGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbeI
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIII NlaIV RsaI SacII
Sau96I ScrFI SfcI SfoI TaqI
TseI TthHB8I