Restriction map for
pF1KB3860
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AseI AsnI AspEI AspHI
AspI AvaI AviII AvrII BbrPI
BbsI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HpaI MaeI
MaeIII MamI MfeI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfaNI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 116 GGTACC G^GTACC
AccI 148 GTCTAC GT^[AC][GT]AC
Aor51HI 920 AGCGCT AGC^GCT
ApaI 136 GGGCCC GGGCC^C
AscI 94 GGCGCGCC GG^CGCGCC
Asp718I 116 GGTACC G^GTACC
BamHI 481 GGATCC G^GATCC
BbeI 991 GGCGCC GGCGC^C
BbvCI 120 CCTCAGC CCTCAGC(-5/-2)
BlpI 1026 GCTCAGC GC^T[ACGT]AGC
BpmI 58 CTGGAG CTGGAG(16/14)
Bpu1102I 1026 GCTCAGC GC^T[ACGT]AGC
BsmI 891 GCATTC GAATGC(1/-1)
BsrI 912 CCAGT ACTGG(1/-1)
BstYI 481 GGATCC [GA]^GATC[CT]
CelII 1026 GCTCAGC GC^T[ACGT]AGC
DpnII 482 GATC ^GATC
DrdI 313 GACGCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 920 AGCGCT AGC^GCT
EcoT14I 707 CCATGG C^C[AT][AT]GG
HinfI 970 GAGTC G^A[ACGT]TC
HphI 356 TCACC GGTGA(8/7)
KasI 991 GGCGCC G^GCGCC
KpnI 116 GGTACC GGTAC^C
KspI 1004 CCGCGG CCGC^GG
MboI 482 GATC ^GATC
MflI 481 GGATCC [GA]^GATC[CT]
MslI 708 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 991 GGCGCC GG^CGCC
NcoI 707 CCATGG C^CATGG
NdeII 482 GATC ^GATC
PleI 970 GAGTC GAGTC(4/5)
PpuMI 7 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 1004 CCGCGG CCGC^GG
SapI 930 GAAGAGC GCTCTTC(1/4)
Sau3AI 482 GATC ^GATC
SfoI 991 GGCGCC GGC^GCC
SgrAI 810 CACCGGCG C[GA]^CCGG[CT]G
SmlI 943 CTCAAG C^T[CT][GA]AG
StuI 848 AGGCCT AGG^CCT
StyI 707 CCATGG C^C[AT][AT]GG
TaqI 610 TCGA T^CGA
TthHB8I 610 TCGA T^CGA
XhoII 481 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 482 GATCC GGATC(4/5)
481 GGATC
Asp700I 819 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAGAGCTTC
AvaII 8 GGTCC G^G[AT]CC
232 GGTCC
BalI 281 TGGCCA TGG^CCA
710 TGGCCA
BanII 136 GGGCCC G[GA]GC[CT]^C
196 GAGCCC
BcgI 83 GCAGCGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
845 GCAAGGCCTTCG
BglI 418 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
497 GCCGGGGCGGC
BmyI 136 GGGCCC G[AGT]GC[ACT]^C
196 GAGCCC
BsaAI 130 TACGTG [CT]AC^GT[GA]
691 TACGTG
BsgI 103 CTGCAC GTGCAG(16/14)
1036 GTGCAG
Bsp1286I 136 GGGCCC G[AGT]GC[ACT]^C
196 GAGCCC
BsrBI 404 CCGCTC CCGCTC(-3/-3)
861 CCGCTC
DsaI 707 CCATGG C^C[GA][CT]GG
1004 CCGCGG
EarI 45 CTCTTC CTCTTC(1/4)
930 GAAGAG
Ksp632I 45 CTCTTC CTCTTC(1/4)
930 GAAGAG
MluI 38 ACGCGT A^CGCGT
572 ACGCGT
MluNI 281 TGGCCA TGG^CCA
710 TGGCCA
MscI 281 TGGCCA TGG^CCA
710 TGGCCA
NspI 797 ACATGC [GA]CATG^[CT]
881 ACATGC
PstI 242 CTGCAG CTGCA^G
787 CTGCAG
PvuII 212 CAGCTG CAG^CTG
655 CAGCTG
SfcI 242 CTGCAG C^T[GA][CT]AG
787 CTGCAG
XcmI 5 CCAGGTCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
698 CCATGCCGGCCATGG
XmnI 819 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI BanI BbvI BcnI
BsaHI BsaJI BsiEI BsiYI BslI
BspMI BsrFI BssHII BssKI BstAPI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV RsaI Sau96I
ScrFI TseI TspRI