Restriction map for
pF1KB3849
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvrII
BbsI BbvCI BciVI BfrI BglI
BglII BlnI BpuAI BsaBI BsiWI
BsmI BspDI BspHI BspLU11I BssSI
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoT22I FseI HindIII HpaI KpnI
MamI MluI NdeI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SbfI ScaI SfiI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1318 GACGTC GACGT^C
AccIII 683 TCCGGA T^CCGGA
Asp700I 1048 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1463 TGGCCA TGG^CCA
BamHI 686 GGATCC G^GATCC
BbeI 10 GGCGCC GGCGC^C
BcgI 1695 CGACGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1091 TGATCA T^GATCA
BseAI 683 TCCGGA T^CCGGA
BsiEI 1647 CGACCG CG[GA][CT]^CG
BspEI 683 TCCGGA T^CCGGA
BspMI 2722 GCAGGT ACCTGC(4/8)
BstBI 1570 TTCGAA TT^CGAA
EcoRI 870 GAATTC G^AATTC
EcoRV 2008 GATATC GAT^ATC
FbaI 1091 TGATCA T^GATCA
KasI 10 GGCGCC G^GCGCC
KspI 1666 CCGCGG CCGC^GG
MfeI 2302 CAATTG C^AATTG
MluNI 1463 TGGCCA TGG^CCA
MroI 683 TCCGGA T^CCGGA
MscI 1463 TGGCCA TGG^CCA
MunI 2302 CAATTG C^AATTG
NaeI 2581 GCCGGC GCC^GGC
NarI 10 GGCGCC GG^CGCC
NcoI 2588 CCATGG C^CATGG
NgoMIV 2581 GCCGGC G^CCGGC
NheI 1819 GCTAGC G^CTAGC
NspV 1570 TTCGAA TT^CGAA
PflMI 2719 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1689 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 1666 CCGCGG CCGC^GG
SalI 2376 GTCGAC G^TCGAC
SfoI 10 GGCGCC GGC^GCC
SfuI 1570 TTCGAA TT^CGAA
SmaI 1828 CCCGGG CCC^GGG
Van91I 2719 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1961 CCACGCTGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1828 CCCGGG C^CCGGG
XmaI 1828 CCCGGG C^CCGGG
XmnI 1048 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2376 GTCGAC GT^[AC][GT]AC
2656 GTCTAC
AflIII 734 ACGTGT A^C[GA][CT]GT
1499 ACGTGT
AlwNI 794 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1906 CAGCGCCTG
AvaI 112 CCCGAG C^[CT]CG[GA]G
1828 CCCGGG
BlpI 1611 GCTCAGC GC^T[ACGT]AGC
2044 GCTGAGC
Bpu1102I 1611 GCTCAGC GC^T[ACGT]AGC
2044 GCTGAGC
BsaWI 683 TCCGGA [AT]^CCGG[AT]
1335 TCCGGT
BsgI 434 CTGCAC GTGCAG(16/14)
1966 CTGCAC
BsmBI 731 GAGACG CGTCTC(1/5)
1316 GAGACG
BsoBI 112 CCCGAG C^[CT]CG[GA]G
1828 CCCGGG
Bsp1407I 737 TGTACA T^GTACA
979 TGTACA
BsrDI 2672 GCAATG GCAATG(2/0)
2729 GCAATG
BsrFI 2476 ACCGGC [GA]^CCGG[CT]
2581 GCCGGC
BsrGI 737 TGTACA T^GTACA
979 TGTACA
BstEII 693 GGTAACC G^GT[ACGT]ACC
1749 GGTGACC
BstPI 693 GGTAACC G^GT[ACGT]ACC
1749 GGTGACC
BstYI 95 AGATCC [GA]^GATC[CT]
686 GGATCC
CelII 1611 GCTCAGC GC^T[ACGT]AGC
2044 GCTGAGC
Cfr10I 2476 ACCGGC [GA]^CCGG[CT]
2581 GCCGGC
EcoO65I 693 GGTAACC G^GT[ACGT]ACC
1749 GGTGACC
HincII 1321 GTCAAC GT[CT]^[GA]AC
2376 GTCGAC
HindII 1321 GTCAAC GT[CT]^[GA]AC
2376 GTCGAC
MflI 95 AGATCC [GA]^GATC[CT]
686 GGATCC
PvuII 1558 CAGCTG CAG^CTG
2693 CAGCTG
SacI 679 GAGCTC GAGCT^C
2559 GAGCTC
SexAI 1745 ACCTGGT A^CC[AT]GGT
2172 ACCTGGT
SspBI 737 TGTACA T^GTACA
979 TGTACA
SspI 929 AATATT AAT^ATT
994 AATATT
Tsp45I 1246 GTCAC ^GT[GC]AC
1750 GTGAC
XhoII 95 AGATCC [GA]^GATC[CT]
686 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AseI AsnI
AspHI AvaII AviII BanI BanII
BbrPI BbvI BcnI BfaI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PmaCI PmlI PshBI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I