Restriction map for
pF1KB3824
##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII Aor51HI ApaI ApoI
AscI Asp700I Asp718I AspI AvrII
BalI BbeI BbvCI BfrI BlnI
BlpI Bpu1102I BseAI BsiWI BsmBI
BspDI BspEI BspHI Bst1107I BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoRI EcoT22I FseI HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PmeI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SexAI SfiI
SfoI SgrAI SnaBI SwaI Tth111I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 172 GGCGTC G[GA]^CG[CT]C
AgeI 1241 ACCGGT A^CCGGT
AhdI 795 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2100 GTGCAC G^TGCAC
AocI 701 CCTCAGG CC^T[ACGT]AGG
ApaLI 2100 GTGCAC G^TGCAC
AseI 1447 ATTAAT AT^TAAT
AsnI 1447 ATTAAT AT^TAAT
AspEI 795 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 653 TGCGCA TGC^GCA
BamHI 916 GGATCC G^GATCC
BbrPI 192 CACGTG CAC^GTG
BcgI 582 CGAGGGTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1919 TGATCA T^GATCA
BsaBI 1834 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 172 GGCGTC G[GA]^CG[CT]C
BsaWI 1241 ACCGGT [AT]^CCGG[AT]
Bsp1407I 2200 TGTACA T^GTACA
BspLU11I 1040 ACATGT A^CATGT
BsrGI 2200 TGTACA T^GTACA
BssHII 82 GCGCGC G^CGCGC
BssSI 938 CTCGTG CACGAG(-5/-1)
BstEII 2752 GGTCACC G^GT[ACGT]ACC
BstPI 2752 GGTCACC G^GT[ACGT]ACC
Bsu36I 701 CCTCAGG CC^T[ACGT]AGG
DraII 2495 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 29 TGGCCG [CT]^GGCC[GA]
Eam1105I 795 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 505 GAAGAG CTCTTC(1/4)
Eco81I 701 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2495 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2752 GGTCACC G^GT[ACGT]ACC
EcoRV 2725 GATATC GAT^ATC
FbaI 1919 TGATCA T^GATCA
FspI 653 TGCGCA TGC^GCA
HaeII 99 GGCGCT [GA]GCGC^[CT]
Hin1I 172 GGCGTC G[GA]^CG[CT]C
Ksp632I 505 GAAGAG CTCTTC(1/4)
MamI 1834 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 31 GCCGGC GCC^GGC
NgoMIV 31 GCCGGC G^CCGGC
PinAI 1241 ACCGGT A^CCGGT
PmaCI 192 CACGTG CAC^GTG
PmlI 192 CACGTG CAC^GTG
PpuMI 2495 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1447 ATTAAT AT^TAAT
SbfI 2526 CCTGCAGG CCTGCA^GG
ScaI 2020 AGTACT AGT^ACT
SpeI 687 ACTAGT A^CTAGT
SphI 3059 GCATGC GCATG^C
Sse8387I 2526 CCTGCAGG CCTGCA^GG
SspBI 2200 TGTACA T^GTACA
SspI 1025 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 191 ACACGT A^C[GA][CT]GT
1040 ACATGT
AlwNI 144 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
621 CAGCCGCTG
AvaI 95 CCCGGG C^[CT]CG[GA]G
153 CCCGGG
AvaII 1481 GGACC G^G[AT]CC
2496 GGACC
BglI 1076 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2525 GCCTGCAGGGC
BglII 1583 AGATCT A^GATCT
1721 AGATCT
BsaAI 192 CACGTG [CT]AC^GT[GA]
1138 TACGTG
BsaI 1238 GAGACC GGTCTC(1/5)
51 GGTCTC
BsoBI 95 CCCGGG C^[CT]CG[GA]G
153 CCCGGG
BsrBI 283 CCGCTC CCGCTC(-3/-3)
2488 GAGCGG
BsrDI 2817 CATTGC GCAATG(2/0)
374 GCAATG
BsrFI 31 GCCGGC [GA]^CCGG[CT]
1241 ACCGGT
BstBI 434 TTCGAA TT^CGAA
1046 TTCGAA
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
1241 ACCGGT
DraI 364 TTTAAA TTT^AAA
489 TTTAAA
DsaI 1233 CCACGG C^C[GA][CT]GG
1829 CCGTGG
HgaI 173 GCGTC GACGC(5/10)
1277 GCGTC
HincII 1279 GTCAAC GT[CT]^[GA]AC
2683 GTTGAC
HindII 1279 GTCAAC GT[CT]^[GA]AC
2683 GTTGAC
NspV 434 TTCGAA TT^CGAA
1046 TTCGAA
PshAI 1054 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1678 GACATCTGTC
SacI 1705 GAGCTC GAGCT^C
2014 GAGCTC
SfuI 434 TTCGAA TT^CGAA
1046 TTCGAA
SmaI 95 CCCGGG CCC^GGG
153 CCCGGG
SmlI 824 CTCAAG C^T[CT][GA]AG
2394 CTTGAG
StuI 1781 AGGCCT AGG^CCT
2471 AGGCCT
XcmI 2902 CCACTGCTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2984 CCAGAGTCCAGGTGG
XmaCI 95 CCCGGG C^CCGGG
153 CCCGGG
XmaI 95 CCCGGG C^CCGGG
153 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII