Restriction map for
pF1KB3766
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsiYI BslI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MluI MluNI MroI MscI
MvnI NarI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 1498 AAATTC [GA]^AATT[CT]
AlwNI 1437 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1498 AAATTC [GA]^AATT[CT]
AvaII 1483 GGACC G^G[AT]CC
AvrII 610 CCTAGG C^CTAGG
BanI 1262 GGCACC G^G[CT][GA]CC
BclI 1116 TGATCA T^GATCA
BcnI 10 CCCGG CC^[GC]GG
BglI 26 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 610 CCTAGG C^CTAGG
BsaI 707 GAGACC GGTCTC(1/5)
BsgI 266 CTGCAC GTGCAG(16/14)
BsmFI 720 GTCCC GGGAC(10/14)
BsmI 1020 GAATGC GAATGC(1/-1)
BspHI 881 TCATGA T^CATGA
BsrDI 900 CATTGC GCAATG(2/0)
BsrFI 26 GCCGGC [GA]^CCGG[CT]
BstAPI 462 GCAGCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 196 GGTGACC G^GT[ACGT]ACC
BstPI 196 GGTGACC G^GT[ACGT]ACC
BstXI 23 CCAGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
EarI 826 GAAGAG CTCTTC(1/4)
EcoO65I 196 GGTGACC G^GT[ACGT]ACC
EcoT14I 610 CCTAGG C^C[AT][AT]GG
FbaI 1116 TGATCA T^GATCA
HgaI 640 GCGTC GACGC(5/10)
HphI 196 GGTGA GGTGA(8/7)
Ksp632I 826 GAAGAG CTCTTC(1/4)
MfeI 295 CAATTG C^AATTG
MslI 1253 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 295 CAATTG C^AATTG
NaeI 26 GCCGGC GCC^GGC
NciI 10 CCCGG CC^[GC]GG
NgoMIV 26 GCCGGC G^CCGGC
NheI 156 GCTAGC G^CTAGC
PleI 817 GAGTC GAGTC(4/5)
RcaI 881 TCATGA T^CATGA
SacI 829 GAGCTC GAGCT^C
SapI 826 GAAGAGC GCTCTTC(1/4)
StuI 1043 AGGCCT AGG^CCT
StyI 610 CCTAGG C^C[AT][AT]GG
SwaI 1494 ATTTAAAT ATTT^AAAT
XbaI 1137 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 75 GTAC GT^AC
693 GTAC
Asp700I 1072 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1308 GAAAAAGTTC
AspHI 391 GTGCTC G[AT]GC[AT]^C
829 GAGCTC
BanII 33 GGGCTC G[GA]GC[CT]^C
829 GAGCTC
BsaJI 9 CCCCGG C^C[ACGT][ACGT]GG
610 CCTAGG
BsiHKAI 391 GTGCTC G[AT]GC[AT]^C
829 GAGCTC
BsmAI 707 GAGAC GTCTC(1/5)
435 GTCTC
BstF5I 558 GGATG GGATG(2/0)
1395 GGATG
BstNI 60 CCTGG CC^[AT]GG
756 CCAGG
BstYI 66 GGATCT [GA]^GATC[CT]
1386 AGATCC
Cfr13I 6 GGCCC G^G[ACGT]CC
1483 GGACC
DraI 382 TTTAAA TTT^AAA
1495 TTTAAA
EcoRII 60 CCTGG ^CC[AT]GG
756 CCAGG
FokI 558 GGATG GGATG(9/13)
1395 GGATG
HaeIII 6 GGCC GG^CC
1044 GGCC
HapII 11 CCGG C^CGG
27 CCGG
HpaII 11 CCGG C^CGG
27 CCGG
MflI 66 GGATCT [GA]^GATC[CT]
1386 AGATCC
MspI 11 CCGG C^CGG
27 CCGG
MvaI 60 CCTGG CC^[AT]GG
756 CCAGG
RsaI 75 GTAC GT^AC
693 GTAC
Sau96I 6 GGCCC G^G[ACGT]CC
1483 GGACC
XhoII 66 GGATCT [GA]^GATC[CT]
1386 AGATCC
XmnI 1072 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1308 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BmyI Bsp1286I BssKI Cac8I DdeI
DpnII Fnu4HI HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MwoI NdeII NlaIII NlaIV
PstI PvuII Sau3AI ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I