Restriction map for
pF1KB3736
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AvrII BamHI
BanI BbeI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 679 GTCTAC GT^[AC][GT]AC
AccII 706 CGCG CG^CG
AcsI 223 AAATTT [GA]^AATT[CT]
AcyI 207 GACGCC G[GA]^CG[CT]C
AhdI 1161 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1041 GATCC GGATC(4/5)
AlwNI 883 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 416 AGCGCT AGC^GCT
ApaI 809 GGGCCC GGGCC^C
ApoI 223 AAATTT [GA]^AATT[CT]
AspEI 1161 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 455 TGCGCA TGC^GCA
BbrPI 738 CACGTG CAC^GTG
BfaI 1112 CTAG C^TAG
BsaHI 207 GACGCC G[GA]^CG[CT]C
BsaWI 830 ACCGGA [AT]^CCGG[AT]
BsrBI 783 CCGCTC CCGCTC(-3/-3)
BsrDI 176 GCAATG GCAATG(2/0)
BsrFI 1034 ACCGGC [GA]^CCGG[CT]
BstBI 1235 TTCGAA TT^CGAA
BstUI 706 CGCG CG^CG
BstYI 1040 AGATCC [GA]^GATC[CT]
Cfr10I 1034 ACCGGC [GA]^CCGG[CT]
DsaI 515 CCATGG C^C[GA][CT]GG
Eam1105I 1161 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 416 AGCGCT AGC^GCT
FauI 704 CCCGC CCCGC(4/6)
FspI 455 TGCGCA TGC^GCA
HaeII 416 AGCGCT [GA]GCGC^[CT]
Hin1I 207 GACGCC G[GA]^CG[CT]C
HincII 1228 GTTGAC GT[CT]^[GA]AC
HindII 1228 GTTGAC GT[CT]^[GA]AC
MaeI 1112 CTAG C^TAG
MflI 1040 AGATCC [GA]^GATC[CT]
MvnI 706 CGCG CG^CG
NcoI 515 CCATGG C^CATGG
NspI 689 GCATGT [GA]CATG^[CT]
NspV 1235 TTCGAA TT^CGAA
PmaCI 738 CACGTG CAC^GTG
PmlI 738 CACGTG CAC^GTG
SfuI 1235 TTCGAA TT^CGAA
SnaBI 427 TACGTA TAC^GTA
StuI 506 AGGCCT AGG^CCT
TaqI 1236 TCGA T^CGA
Tsp509I 224 AATT ^AATT
TthHB8I 1236 TCGA T^CGA
XcmI 318 CCACGCCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1040 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BbsI 229 GTCTTC GAAGAC(2/6)
235 GTCTTC
BbvCI 1101 GCTGAGG CCTCAGC(-5/-2)
1255 GCTGAGG
BglI 703 GCCCGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1115 GCCGGGAGGGC
BlpI 940 GCTCAGC GC^T[ACGT]AGC
1053 GCTCAGC
Bpu1102I 940 GCTCAGC GC^T[ACGT]AGC
1053 GCTCAGC
BpuAI 229 GTCTTC GAAGAC(2/6)
235 GTCTTC
BsaAI 427 TACGTA [CT]AC^GT[GA]
738 CACGTG
BsaI 1261 GAGACC GGTCTC(1/5)
543 GGTCTC
BstEII 795 GGTCACC G^GT[ACGT]ACC
975 GGTCACC
BstPI 795 GGTCACC G^GT[ACGT]ACC
975 GGTCACC
BstXI 404 CCATCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
737 CCACGTGGATGG
CelII 940 GCTCAGC GC^T[ACGT]AGC
1053 GCTCAGC
DpnII 198 GATC ^GATC
1041 GATC
EarI 36 CTCTTC CTCTTC(1/4)
136 CTCTTC
EcoO65I 795 GGTCACC G^GT[ACGT]ACC
975 GGTCACC
EcoT14I 509 CCTTGG C^C[AT][AT]GG
515 CCATGG
HgaI 207 GACGC GACGC(5/10)
462 GACGC
Ksp632I 36 CTCTTC CTCTTC(1/4)
136 CTCTTC
MaeIII 796 GTCAC ^GT[ACGT]AC
976 GTCAC
MboI 198 GATC ^GATC
1041 GATC
MspA1I 182 CCGCTG C[AC]G^C[GT]G
730 CCGCTG
NdeII 198 GATC ^GATC
1041 GATC
PstI 684 CTGCAG CTGCA^G
1144 CTGCAG
SacI 560 GAGCTC GAGCT^C
1051 GAGCTC
Sau3AI 198 GATC ^GATC
1041 GATC
SfcI 684 CTGCAG C^T[GA][CT]AG
1144 CTGCAG
StyI 509 CCTTGG C^C[AT][AT]GG
515 CCATGG
Tsp45I 796 GTCAC ^GT[GC]AC
976 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboII MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NlaIII NlaIV PleI PpuMI
Sau96I ScrFI TfiI TseI TspRI