Restriction map for
pF1KB3726
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmI BspEI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I DraI DraIII EagI
EclXI Eco52I EcoNI EcoRI EcoT22I
FbaI FseI FspI HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 2476 GACGTC GACGT^C
AflIII 680 ACACGT A^C[GA][CT]GT
Alw44I 1105 GTGCAC G^TGCAC
Aor51HI 1967 AGCGCT AGC^GCT
ApaI 115 GGGCCC GGGCC^C
ApaLI 1105 GTGCAC G^TGCAC
BanI 1977 GGTGCC G^G[CT][GA]CC
BfaI 742 CTAG C^TAG
BglI 14 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1143 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 600 GTGCAG GTGCAG(16/14)
BspDI 148 ATCGAT AT^CGAT
BsrFI 1924 ACCGGC [GA]^CCGG[CT]
BstEII 501 GGTCACC G^GT[ACGT]ACC
BstPI 501 GGTCACC G^GT[ACGT]ACC
Cfr10I 1924 ACCGGC [GA]^CCGG[CT]
ClaI 148 ATCGAT AT^CGAT
CpoI 2201 CGGACCG CG^G[AT]CCG
DrdI 493 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1967 AGCGCT AGC^GCT
EcoO65I 501 GGTCACC G^GT[ACGT]ACC
EcoRV 2049 GATATC GAT^ATC
HincII 1735 GTCAAC GT[CT]^[GA]AC
HindII 1735 GTCAAC GT[CT]^[GA]AC
HindIII 1441 AAGCTT A^AGCTT
MaeI 742 CTAG C^TAG
MamI 1143 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
RsrII 2201 CGGACCG CG^G[AT]CCG
SexAI 1061 ACCTGGT A^CC[AT]GGT
XcmI 1287 CCACAATCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 29 CGCG CG^CG
896 CGCG
AcsI 1861 GAATTT [GA]^AATT[CT]
1873 AAATTC
AhdI 1486 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACATCTGGTC
AocI 226 CCTGAGG CC^T[ACGT]AGG
1692 CCTGAGG
ApoI 1861 GAATTT [GA]^AATT[CT]
1873 AAATTC
Asp700I 1239 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1354 GAAGCAATTC
AspEI 1486 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACATCTGGTC
AspI 721 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1905 GACCCCGTC
BlpI 9 GCTCAGC GC^T[ACGT]AGC
177 GCTCAGC
Bpu1102I 9 GCTCAGC GC^T[ACGT]AGC
177 GCTCAGC
BsmBI 210 CGTCTC CGTCTC(1/5)
892 GAGACG
Bsp1407I 548 TGTACA T^GTACA
1452 TGTACA
BsrGI 548 TGTACA T^GTACA
1452 TGTACA
BstUI 29 CGCG CG^CG
896 CGCG
Bsu36I 226 CCTGAGG CC^T[ACGT]AGG
1692 CCTGAGG
CelII 9 GCTCAGC GC^T[ACGT]AGC
177 GCTCAGC
Eam1105I 1486 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACATCTGGTC
Eco81I 226 CCTGAGG CC^T[ACGT]AGG
1692 CCTGAGG
MseI 320 TTAA T^TAA
1547 TTAA
MvnI 29 CGCG CG^CG
896 CGCG
NcoI 506 CCATGG C^CATGG
692 CCATGG
PflFI 721 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1905 GACCCCGTC
PshAI 1728 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1923 GACCGGCGTC
SspBI 548 TGTACA T^GTACA
1452 TGTACA
StuI 261 AGGCCT AGG^CCT
1388 AGGCCT
TfiI 1084 GAATC G^A[AT]TC
1585 GATTC
Tru9I 320 TTAA T^TAA
1547 TTAA
Tth111I 721 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1905 GACCCCGTC
XmnI 1239 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1354 GAAGCAATTC
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BamHI BanII BbsI BbvI BcnI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII