Restriction map for
pF1KB3350
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI Bsp1407I BspDI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SfiI SfoI SfuI SnaBI
SpeI SphI SspBI SspI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1056 CGCG CG^CG
AccIII 1620 TCCGGA T^CCGGA
AflIII 1392 ACATGT A^C[GA][CT]GT
Alw44I 1471 GTGCAC G^TGCAC
ApaI 352 GGGCCC GGGCC^C
ApaLI 1471 GTGCAC G^TGCAC
AspHI 1471 GTGCAC G[AT]GC[AT]^C
AspI 846 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1877 TGCGCA TGC^GCA
BbsI 1117 GAAGAC GAAGAC(2/6)
BbvCI 454 CCTCAGC CCTCAGC(-5/-2)
BglII 1479 AGATCT A^GATCT
BpuAI 1117 GAAGAC GAAGAC(2/6)
BsaWI 1620 TCCGGA [AT]^CCGG[AT]
BseAI 1620 TCCGGA T^CCGGA
BsiHKAI 1471 GTGCAC G[AT]GC[AT]^C
BsmAI 590 GAGAC GTCTC(1/5)
BspEI 1620 TCCGGA T^CCGGA
BspHI 1811 TCATGA T^CATGA
BspLU11I 1392 ACATGT A^CATGT
BstAPI 1552 GCACTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1056 CGCG CG^CG
BstXI 1584 CCAGACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1479 AGATCT [GA]^GATC[CT]
DraIII 1553 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 1626 ATGCAT ATGCA^T
FspI 1877 TGCGCA TGC^GCA
HaeII 25 AGCGCC [GA]GCGC^[CT]
HgaI 1110 GACGC GACGC(5/10)
MaeII 1895 ACGT A^CGT
MflI 1479 AGATCT [GA]^GATC[CT]
MroI 1620 TCCGGA T^CCGGA
MvnI 1056 CGCG CG^CG
NsiI 1626 ATGCAT ATGCA^T
NspI 1392 ACATGT [GA]CATG^[CT]
PflFI 846 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 895 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 238 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 1811 TCATGA T^CATGA
SexAI 83 ACCTGGT A^CC[AT]GGT
SgrAI 228 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 62 CCCGGG CCC^GGG
Tsp45I 1741 GTCAC ^GT[GC]AC
Tth111I 846 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 895 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1479 AGATCT [GA]^GATC[CT]
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 579 GATCC GGATC(4/5)
553 GGATC
AlwNI 421 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1273 CAGCAGCTG
Asp700I 312 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1417 GAACTGGTTC
BalI 2 TGGCCA TGG^CCA
903 TGGCCA
BanI 214 GGCACC G^G[CT][GA]CC
1775 GGTGCC
BfaI 718 CTAG C^TAG
1504 CTAG
BsmFI 769 GTCCC GGGAC(10/14)
1361 GTCCC
BsmI 1624 GAATGC GAATGC(1/-1)
1693 GCATTC
BspMI 35 ACCTGC ACCTGC(4/8)
410 ACCTGC
DdeI 455 CTCAG C^T[ACGT]AG
533 CTGAG
EaeI 2 TGGCCA [CT]^GGCC[GA]
903 TGGCCA
EcoNI 84 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
922 CCTGCCCAAGG
MaeI 718 CTAG C^TAG
1504 CTAG
MaeIII 1507 GTTAC ^GT[ACGT]AC
1741 GTCAC
MluNI 2 TGGCCA TGG^CCA
903 TGGCCA
MscI 2 TGGCCA TGG^CCA
903 TGGCCA
PleI 283 GAGTC GAGTC(4/5)
1324 GAGTC
PvuII 457 CAGCTG CAG^CTG
1276 CAGCTG
SbfI 411 CCTGCAGG CCTGCA^GG
1780 CCTGCAGG
ScaI 946 AGTACT AGT^ACT
1448 AGTACT
SmlI 307 CTCAAG C^T[CT][GA]AG
870 CTCAAG
Sse8387I 411 CCTGCAGG CCTGCA^GG
1780 CCTGCAGG
XcmI 5 CCAGCAGCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
279 CCAGGAGTCTGATGG
XmnI 312 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1417 GAACTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I