Restriction map for
pF1KB3332
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AscI Asp718I AviII
BalI BbeI BbrPI BciVI BclI
BsaAI BsaHI BseAI BsiWI BspEI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I CpoI DraI DrdI EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MfeI MluI MluNI
MroI MscI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 2800 GAATTT [GA]^AATT[CT]
AflII 1417 CTTAAG C^TTAAG
AflIII 1559 ACGTGT A^C[GA][CT]GT
Alw44I 2924 GTGCAC G^TGCAC
Aor51HI 3087 AGCGCT AGC^GCT
ApaLI 2924 GTGCAC G^TGCAC
ApoI 2800 GAATTT [GA]^AATT[CT]
AseI 2641 ATTAAT AT^TAAT
AsnI 2641 ATTAAT AT^TAAT
Asp700I 2488 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1184 GGATCC G^GATCC
BanI 2413 GGTGCC G^G[CT][GA]CC
BfrI 1417 CTTAAG C^TTAAG
BglII 2330 AGATCT A^GATCT
BsaBI 1185 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1194 CGGCCG CG[GA][CT]^CG
Bsp1407I 1674 TGTACA T^GTACA
BspDI 3337 ATCGAT AT^CGAT
BsrBI 180 CCGCTC CCGCTC(-3/-3)
BsrDI 1544 GCAATG GCAATG(2/0)
BsrFI 1166 GCCGGC [GA]^CCGG[CT]
BsrGI 1674 TGTACA T^GTACA
BssSI 1710 CACGAG CACGAG(-5/-1)
BstEII 1872 GGTGACC G^GT[ACGT]ACC
BstPI 1872 GGTGACC G^GT[ACGT]ACC
BstXI 525 CCACCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1166 GCCGGC [GA]^CCGG[CT]
ClaI 3337 ATCGAT AT^CGAT
DraIII 379 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1194 CGGCCG C^GGCCG
EclXI 1194 CGGCCG C^GGCCG
Eco47III 3087 AGCGCT AGC^GCT
Eco52I 1194 CGGCCG C^GGCCG
EcoO65I 1872 GGTGACC G^GT[ACGT]ACC
MamI 1185 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1166 GCCGGC GCC^GGC
NcoI 1919 CCATGG C^CATGG
NgoMIV 1166 GCCGGC G^CCGGC
PflFI 1648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 696 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2641 ATTAAT AT^TAAT
SapI 3166 GAAGAGC GCTCTTC(1/4)
SexAI 1649 ACCTGGT A^CC[AT]GGT
SspBI 1674 TGTACA T^GTACA
TfiI 1240 GATTC G^A[AT]TC
Tth111I 1648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 696 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2488 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 60 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1132 GACCTGCTGTC
AlwI 1185 GATCC GGATC(4/5)
1184 GGATC
AspEI 60 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1132 GACCTGCTGTC
BbsI 2722 GAAGAC GAAGAC(2/6)
2881 GAAGAC
BcgI 1557 CGACGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
76 GCAGTGAGCTCG
BglI 2373 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3319 GCCATGCTGGC
BlpI 975 GCTCAGC GC^T[ACGT]AGC
1386 GCTCAGC
Bpu1102I 975 GCTCAGC GC^T[ACGT]AGC
1386 GCTCAGC
BpuAI 2722 GAAGAC GAAGAC(2/6)
2881 GAAGAC
BsaI 67 GGTCTC GGTCTC(1/5)
1845 GGTCTC
BsaWI 162 TCCGGT [AT]^CCGG[AT]
1529 ACCGGA
BsmBI 292 CGTCTC CGTCTC(1/5)
1769 CGTCTC
BsmI 1340 GAATGC GAATGC(1/-1)
401 GCATTC
BstAPI 813 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
844 GCAGAGACTGC
BstYI 1184 GGATCC [GA]^GATC[CT]
2330 AGATCT
CelII 975 GCTCAGC GC^T[ACGT]AGC
1386 GCTCAGC
Eam1105I 60 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1132 GACCTGCTGTC
HgaI 2559 GACGC GACGC(5/10)
1049 GCGTC
MaeII 291 ACGT A^CGT
1559 ACGT
MflI 1184 GGATCC [GA]^GATC[CT]
2330 AGATCT
MseI 1418 TTAA T^TAA
2642 TTAA
SspI 1063 AATATT AAT^ATT
2756 AATATT
TaqI 141 TCGA T^CGA
3338 TCGA
Tru9I 1418 TTAA T^TAA
2642 TTAA
Tsp509I 1219 AATT ^AATT
2801 AATT
TthHB8I 141 TCGA T^CGA
3338 TCGA
XcmI 437 CCACAATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2009 CCATGCCCTCAGTGG
XhoII 1184 GGATCC [GA]^GATC[CT]
2330 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
AvrII BanII BbvCI BbvI BcnI
BfaI BlnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TseI Tsp45I TspRI XmaCI XmaI