Restriction map for
pF1KB3327
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AflII Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspHI AspI AvaI AviII
BalI BbeI BbrPI BcgI BciVI
BfrI BglII BsaAI BsaBI BseAI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
BstAPI BstBI ClaI CpoI DraI
Eco47III EcoRV EcoT22I FseI FspI
HaeII HindIII HpaI KasI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 37 GGTACC G^GTACC
AccI 2610 GTATAC GT^[AC][GT]AC
AgeI 1258 ACCGGT A^CCGGT
Asp700I 1927 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 37 GGTACC G^GTACC
AvrII 486 CCTAGG C^CTAGG
BbvCI 1757 CCTCAGC CCTCAGC(-5/-2)
BglI 485 GCCTAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 486 CCTAGG C^CTAGG
BlpI 262 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 262 GCTGAGC GC^T[ACGT]AGC
BsaWI 1258 ACCGGT [AT]^CCGG[AT]
BsmBI 2138 CGTCTC CGTCTC(1/5)
BsmFI 417 GTCCC GGGAC(10/14)
BspHI 1788 TCATGA T^CATGA
BssSI 1892 CACGAG CACGAG(-5/-1)
Bst1107I 2610 GTATAC GTA^TAC
BstEII 177 GGTCACC G^GT[ACGT]ACC
BstPI 177 GGTCACC G^GT[ACGT]ACC
BstXI 438 CCACCACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2610 GTATAC GTA^TAC
CelII 262 GCTGAGC GC^T[ACGT]AGC
CfoI 1092 GCGC GCG^C
DraIII 1013 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 177 GGTCACC G^GT[ACGT]ACC
EcoRI 1802 GAATTC G^AATTC
HhaI 1092 GCGC GCG^C
HinP1I 1092 GCGC G^CGC
HincII 2274 GTTGAC GT[CT]^[GA]AC
HindII 2274 GTTGAC GT[CT]^[GA]AC
KpnI 37 GGTACC GGTAC^C
NcoI 618 CCATGG C^CATGG
NdeI 991 CATATG CA^TATG
NheI 2431 GCTAGC G^CTAGC
PinAI 1258 ACCGGT A^CCGGT
RcaI 1788 TCATGA T^CATGA
SpeI 1126 ACTAGT A^CTAGT
SspI 1879 AATATT AAT^ATT
StuI 483 AGGCCT AGG^CCT
XmnI 1927 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 324 CGCG CG^CG
396 CGCG
AcyI 315 GGCGTC G[GA]^CG[CT]C
514 GGCGTC
AflIII 834 ACATGT A^C[GA][CT]GT
1096 ACATGT
AhdI 1536 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1673 GACACATGGTC
AlwNI 1133 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
2287 CAGGAGCTG
AocI 403 CCTGAGG CC^T[ACGT]AGG
1910 CCTCAGG
AspEI 1536 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1673 GACACATGGTC
BamHI 399 GGATCC G^GATCC
1869 GGATCC
BanII 265 GAGCCC G[GA]GC[CT]^C
1950 GAGCCC
BclI 789 TGATCA T^GATCA
957 TGATCA
BmyI 265 GAGCCC G[AGT]GC[ACT]^C
1950 GAGCCC
BpmI 479 CTCCAG CTGGAG(16/14)
461 CTGGAG
BsaHI 315 GGCGTC G[GA]^CG[CT]C
514 GGCGTC
Bsp1286I 265 GAGCCC G[AGT]GC[ACT]^C
1950 GAGCCC
BspLU11I 834 ACATGT A^CATGT
1096 ACATGT
BspMI 824 ACCTGC ACCTGC(4/8)
1708 GCAGGT
BsrDI 2192 CATTGC GCAATG(2/0)
222 GCAATG
BstUI 324 CGCG CG^CG
396 CGCG
Bsu36I 403 CCTGAGG CC^T[ACGT]AGG
1910 CCTCAGG
DraII 343 AGGACCT [GA]G^G[ACGT]CC[CT]
524 AGGACCT
DrdI 643 GACACTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2586 GACTTCAGTGTC
Eam1105I 1536 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1673 GACACATGGTC
EarI 469 GAAGAG CTCTTC(1/4)
1377 GAAGAG
Eco81I 403 CCTGAGG CC^T[ACGT]AGG
1910 CCTCAGG
EcoNI 335 CCTCGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2013 CCTCCAACAGG
EcoO109I 343 AGGACCT [GA]G^G[ACGT]CC[CT]
524 AGGACCT
EcoT14I 486 CCTAGG C^C[AT][AT]GG
618 CCATGG
FbaI 789 TGATCA T^GATCA
957 TGATCA
HgaI 316 GCGTC GACGC(5/10)
515 GCGTC
Hin1I 315 GGCGTC G[GA]^CG[CT]C
514 GGCGTC
Ksp632I 469 GAAGAG CTCTTC(1/4)
1377 GAAGAG
KspI 323 CCGCGG CCGC^GG
395 CCGCGG
MvnI 324 CGCG CG^CG
396 CGCG
NaeI 195 GCCGGC GCC^GGC
309 GCCGGC
NgoMIV 195 GCCGGC G^CCGGC
309 GCCGGC
PflMI 141 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2286 CCAGGAGCTGG
PpuMI 343 AGGACCT [GA]G^G[AT]CC[CT]
524 AGGACCT
PstI 1372 CTGCAG CTGCA^G
1706 CTGCAG
SacII 323 CCGCGG CCGC^GG
395 CCGCGG
StyI 486 CCTAGG C^C[AT][AT]GG
618 CCATGG
Van91I 141 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2286 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbsI BbvI
BcnI BfaI BpuAI BsaI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII