Restriction map for
pF1KB3321
##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BbrPI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI PacI PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI SfiI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 2339 TCCGGA T^CCGGA
AhdI 428 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 366 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2114 AGCGCT AGC^GCT
AspEI 428 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2373 GGATCC G^GATCC
BbeI 1114 GGCGCC GGCGC^C
BcgI 306 GCAGCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2039 GTATCC GTATCC(6/5)
BglII 1391 AGATCT A^GATCT
BsaI 350 GAGACC GGTCTC(1/5)
BseAI 2339 TCCGGA T^CCGGA
BsmBI 1885 CGTCTC CGTCTC(1/5)
BsmI 1575 GAATGC GAATGC(1/-1)
BspEI 2339 TCCGGA T^CCGGA
BssSI 2620 CACGAG CACGAG(-5/-1)
BstBI 662 TTCGAA TT^CGAA
Bsu36I 366 CCTTAGG CC^T[ACGT]AGG
Eam1105I 428 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2114 AGCGCT AGC^GCT
Eco81I 366 CCTTAGG CC^T[ACGT]AGG
EcoT22I 1577 ATGCAT ATGCA^T
KasI 1114 GGCGCC G^GCGCC
KspI 76 CCGCGG CCGC^GG
MroI 2339 TCCGGA T^CCGGA
MseI 2216 TTAA T^TAA
NarI 1114 GGCGCC GG^CGCC
NsiI 1577 ATGCAT ATGCA^T
NspI 2650 ACATGC [GA]CATG^[CT]
NspV 662 TTCGAA TT^CGAA
PaeR7I 2359 CTCGAG C^TCGAG
PpuMI 1991 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 988 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 76 CCGCGG CCGC^GG
SapI 691 GAAGAGC GCTCTTC(1/4)
ScaI 2144 AGTACT AGT^ACT
SfoI 1114 GGCGCC GGC^GCC
SfuI 662 TTCGAA TT^CGAA
SpeI 2493 ACTAGT A^CTAGT
StuI 272 AGGCCT AGG^CCT
Tru9I 2216 TTAA T^TAA
XhoI 2359 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1155 GTATAC GT^[AC][GT]AC
2395 GTATAC
AcyI 640 GACGCC G[GA]^CG[CT]C
1114 GGCGCC
AgeI 545 ACCGGT A^CCGGT
1151 ACCGGT
BbsI 985 GAAGAC GAAGAC(2/6)
1279 GTCTTC
BglI 1533 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1608 GCCTGTTTGGC
BpuAI 985 GAAGAC GAAGAC(2/6)
1279 GTCTTC
BsaHI 640 GACGCC G[GA]^CG[CT]C
1114 GGCGCC
BsrBI 283 GAGCGG CCGCTC(-3/-3)
2320 GAGCGG
Bst1107I 1155 GTATAC GTA^TAC
2395 GTATAC
BstAPI 1389 GCAGATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1819 GCAGGTGATGC
BstEII 879 GGTGACC G^GT[ACGT]ACC
900 GGTGACC
BstPI 879 GGTGACC G^GT[ACGT]ACC
900 GGTGACC
BstZ17I 1155 GTATAC GTA^TAC
2395 GTATAC
EarI 2029 CTCTTC CTCTTC(1/4)
691 GAAGAG
EcoO65I 879 GGTGACC G^GT[ACGT]ACC
900 GGTGACC
Hin1I 640 GACGCC G[GA]^CG[CT]C
1114 GGCGCC
Ksp632I 2029 CTCTTC CTCTTC(1/4)
691 GAAGAG
MslI 1321 CACTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2490 CACACTAGTG
NaeI 1117 GCCGGC GCC^GGC
1694 GCCGGC
NcoI 885 CCATGG C^CATGG
1208 CCATGG
NgoMIV 1117 GCCGGC G^CCGGC
1694 GCCGGC
PinAI 545 ACCGGT A^CCGGT
1151 ACCGGT
SmlI 2233 CTCAAG C^T[CT][GA]AG
2359 CTCGAG
TaqI 663 TCGA T^CGA
2360 TCGA
TthHB8I 663 TCGA T^CGA
2360 TCGA
XcmI 2099 CCACCGCCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2683 CCAGCCAGCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
StyI TfiI TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmaCI XmaI