Restriction map for
pF1KB1326
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# NO CUT ENZYMES ( 81 enzymes) #
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AccI AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BciVI
BfrI BsaBI BsiWI BsmI BspDI
BspLU11I BsrDI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI MamI
MfeI MluI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SfuI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tru9I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 268 GACGTC GACGT^C
Acc65I 1982 GGTACC G^GTACC
AflIII 1235 ACGTGT A^C[GA][CT]GT
AocI 2722 CCTAAGG CC^T[ACGT]AGG
Aor51HI 838 AGCGCT AGC^GCT
Asp718I 1982 GGTACC G^GTACC
AvrII 2821 CCTAGG C^CTAGG
BamHI 314 GGATCC G^GATCC
BbrPI 307 CACGTG CAC^GTG
BbvCI 2736 GCTGAGG CCTCAGC(-5/-2)
BclI 2702 TGATCA T^GATCA
BfaI 2822 CTAG C^TAG
BlnI 2821 CCTAGG C^CTAGG
BlpI 2259 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2259 GCTGAGC GC^T[ACGT]AGC
BsaI 1808 GAGACC GGTCTC(1/5)
BsmBI 1494 CGTCTC CGTCTC(1/5)
BspHI 288 TCATGA T^CATGA
BstAPI 494 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2722 CCTAAGG CC^T[ACGT]AGG
CelII 2259 GCTGAGC GC^T[ACGT]AGC
Eco47III 838 AGCGCT AGC^GCT
Eco81I 2722 CCTAAGG CC^T[ACGT]AGG
FbaI 2702 TGATCA T^GATCA
KpnI 1982 GGTACC GGTAC^C
MaeI 2822 CTAG C^TAG
PflMI 1332 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 307 CACGTG CAC^GTG
PmlI 307 CACGTG CAC^GTG
RcaI 288 TCATGA T^CATGA
SapI 1257 GAAGAGC GCTCTTC(1/4)
SexAI 2503 ACCAGGT A^CC[AT]GGT
SfiI 1795 GGCCTCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 431 CACCGGCG C[GA]^CCGG[CT]G
TfiI 2603 GAATC G^A[AT]TC
Tsp509I 277 AATT ^AATT
Van91I 1332 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Alw44I 321 GTGCAC G^TGCAC
2728 GTGCAC
ApaLI 321 GTGCAC G^TGCAC
2728 GTGCAC
BalI 1026 TGGCCA TGG^CCA
2012 TGGCCA
BbeI 826 GGCGCC GGCGC^C
1963 GGCGCC
BbsI 603 GAAGAC GAAGAC(2/6)
1852 GTCTTC
BcgI 1987 CGACTGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2240 GCATTGGCATCG
BglI 1796 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2161 GCCCACGAGGC
BglII 902 AGATCT A^GATCT
1199 AGATCT
BpuAI 603 GAAGAC GAAGAC(2/6)
1852 GTCTTC
BsiEI 11 CGGCCG CG[GA][CT]^CG
1302 CGGCCG
Bsp1407I 1664 TGTACA T^GTACA
2567 TGTACA
BsrGI 1664 TGTACA T^GTACA
2567 TGTACA
BssSI 324 CACGAG CACGAG(-5/-1)
2164 CACGAG
EagI 11 CGGCCG C^GGCCG
1302 CGGCCG
EclXI 11 CGGCCG C^GGCCG
1302 CGGCCG
Eco52I 11 CGGCCG C^GGCCG
1302 CGGCCG
KasI 826 GGCGCC G^GCGCC
1963 GGCGCC
MluNI 1026 TGGCCA TGG^CCA
2012 TGGCCA
MscI 1026 TGGCCA TGG^CCA
2012 TGGCCA
NarI 826 GGCGCC GG^CGCC
1963 GGCGCC
NspI 1667 ACATGC [GA]CATG^[CT]
2732 ACATGC
SfoI 826 GGCGCC GGC^GCC
1963 GGCGCC
SspBI 1664 TGTACA T^GTACA
2567 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MnlI MroI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PshAI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII
XmaCI XmaI