Restriction map for
pF1KB7709
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1099 GTCGAC GT^[AC][GT]AC
AcsI 125 AAATTC [GA]^AATT[CT]
AlwNI 767 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 125 AAATTC [GA]^AATT[CT]
AseI 492 ATTAAT AT^TAAT
AsnI 492 ATTAAT AT^TAAT
BbsI 684 GAAGAC GAAGAC(2/6)
BclI 159 TGATCA T^GATCA
BpuAI 684 GAAGAC GAAGAC(2/6)
BsaI 714 GAGACC GGTCTC(1/5)
BsgI 1063 GTGCAG GTGCAG(16/14)
BsmI 279 GCATTC GAATGC(1/-1)
BsrDI 911 GCAATG GCAATG(2/0)
BstAPI 565 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 907 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 698 GCGC GCG^C
DrdI 800 GACGAGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 457 GAAGAG CTCTTC(1/4)
EcoT14I 137 CCTTGG C^C[AT][AT]GG
FbaI 159 TGATCA T^GATCA
HaeII 697 GGCGCT [GA]GCGC^[CT]
HapII 600 CCGG C^CGG
HgaI 273 GACGC GACGC(5/10)
HhaI 698 GCGC GCG^C
HinP1I 698 GCGC G^CGC
HpaII 600 CCGG C^CGG
Ksp632I 457 GAAGAG CTCTTC(1/4)
MseI 493 TTAA T^TAA
MspI 600 CCGG C^CGG
NdeI 212 CATATG CA^TATG
PflMI 132 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 492 ATTAAT AT^TAAT
SalI 1099 GTCGAC G^TCGAC
ScaI 223 AGTACT AGT^ACT
SfaNI 241 GCATC GCATC(5/9)
SmlI 679 CTTGAG C^T[CT][GA]AG
SspI 1024 AATATT AAT^ATT
StyI 137 CCTTGG C^C[AT][AT]GG
TfiI 864 GAATC G^A[AT]TC
Tru9I 493 TTAA T^TAA
Van91I 132 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 602 GGATC GGATC(4/5)
628 GGATC
BfaI 302 CTAG C^TAG
466 CTAG
BglII 81 AGATCT A^GATCT
948 AGATCT
BpmI 819 CTGGAG CTGGAG(16/14)
1042 CTGGAG
BseRI 498 GAGGAG GAGGAG(10/8)
1014 GAGGAG
BsiYI 132 CCATGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
545 CCAATCGGAGG
BslI 132 CCATGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
545 CCAATCGGAGG
HincII 1099 GTCGAC GT[CT]^[GA]AC
1189 GTCAAC
HindII 1099 GTCGAC GT[CT]^[GA]AC
1189 GTCAAC
HphI 298 TCACC GGTGA(8/7)
397 TCACC
MaeI 302 CTAG C^TAG
466 CTAG
NlaIV 738 GGACCC GG[ACGT]^[ACGT]CC
1222 GGAGCC
PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT]
737 AGGACCC
SfcI 251 CTACAG C^T[GA][CT]AG
257 CTACAG
TaqI 810 TCGA T^CGA
1100 TCGA
TthHB8I 810 TCGA T^CGA
1100 TCGA
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII PleI RsaI Sau3AI
Sau96I ScrFI TseI Tsp45I Tsp509I
TspRI XhoII