Restriction map for
pF1KA1531
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccIII AclI AflIII AgeI AhdI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BbrPI BclI BlnI
BsaAI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BstBI
ClaI CpoI DraI DrdI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MfeI MluI
MroI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SbfI
ScaI SexAI SfuI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 3286 GACGTC GACGT^C
Acc65I 842 GGTACC G^GTACC
AcsI 1414 AAATTT [GA]^AATT[CT]
AflII 544 CTTAAG C^TTAAG
AlwNI 679 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2474 AGCGCT AGC^GCT
ApoI 1414 AAATTT [GA]^AATT[CT]
Asp718I 842 GGTACC G^GTACC
BciVI 1146 GTATCC GTATCC(6/5)
BfrI 544 CTTAAG C^TTAAG
BglII 411 AGATCT A^GATCT
BsaBI 1296 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 299 GCAATG GCAATG(2/0)
BssSI 2583 CTCGTG CACGAG(-5/-1)
Bst1107I 1666 GTATAC GTA^TAC
BstAPI 1764 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2415 GGTGACC G^GT[ACGT]ACC
BstPI 2415 GGTGACC G^GT[ACGT]ACC
BstZ17I 1666 GTATAC GTA^TAC
DraIII 1657 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2474 AGCGCT AGC^GCT
EcoO65I 2415 GGTGACC G^GT[ACGT]ACC
KpnI 842 GGTACC GGTAC^C
MamI 1296 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 545 TTAA T^TAA
NcoI 992 CCATGG C^CATGG
NheI 1363 GCTAGC G^CTAGC
SacI 1936 GAGCTC GAGCT^C
SalI 1429 GTCGAC G^TCGAC
SapI 3345 GAAGAGC GCTCTTC(1/4)
SgrAI 1201 CGCCGGTG C[GA]^CCGG[CT]G
SphI 672 GCATGC GCATG^C
TaqI 1430 TCGA T^CGA
Tru9I 545 TTAA T^TAA
Tsp509I 1415 AATT ^AATT
TthHB8I 1430 TCGA T^CGA
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# TWO CUT ENZYMES ( 43 enzymes) #
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AccI 1429 GTCGAC GT^[AC][GT]AC
1666 GTATAC
Alw44I 981 GTGCAC G^TGCAC
2875 GTGCAC
AocI 342 CCTGAGG CC^T[ACGT]AGG
965 CCTCAGG
ApaLI 981 GTGCAC G^TGCAC
2875 GTGCAC
AscI 97 GGCGCGCC GG^CGCGCC
2588 GGCGCGCC
AspI 939 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3283 GACGACGTC
BalI 1469 TGGCCA TGG^CCA
2438 TGGCCA
BbsI 3330 GAAGAC GAAGAC(2/6)
2542 GTCTTC
BbvCI 1578 CCTCAGC CCTCAGC(-5/-2)
2265 GCTGAGG
BcgI 1065 CGAGCGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1535 GCAGCCGCATCG
BfaI 1364 CTAG C^TAG
2404 CTAG
BlpI 159 GCTGAGC GC^T[ACGT]AGC
1611 GCTGAGC
Bpu1102I 159 GCTGAGC GC^T[ACGT]AGC
1611 GCTGAGC
BpuAI 3330 GAAGAC GAAGAC(2/6)
2542 GTCTTC
BsaI 448 GAGACC GGTCTC(1/5)
1039 GAGACC
BseRI 1881 CTCCTC GAGGAG(10/8)
2260 GAGGAG
BsiEI 1218 CGGCCG CG[GA][CT]^CG
2804 CGGCCG
Bsp1407I 1280 TGTACA T^GTACA
1346 TGTACA
BsrGI 1280 TGTACA T^GTACA
1346 TGTACA
BstXI 809 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1625 CCAGGGAGGTGG
BstYI 411 AGATCT [GA]^GATC[CT]
2168 GGATCT
Bsu36I 342 CCTGAGG CC^T[ACGT]AGG
965 CCTCAGG
CelII 159 GCTGAGC GC^T[ACGT]AGC
1611 GCTGAGC
EagI 1218 CGGCCG C^GGCCG
2804 CGGCCG
EclXI 1218 CGGCCG C^GGCCG
2804 CGGCCG
Eco52I 1218 CGGCCG C^GGCCG
2804 CGGCCG
Eco81I 342 CCTGAGG CC^T[ACGT]AGG
965 CCTCAGG
EcoNI 693 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1619 CCTTTCCCAGG
HincII 1429 GTCGAC GT[CT]^[GA]AC
2062 GTCAAC
HindII 1429 GTCGAC GT[CT]^[GA]AC
2062 GTCAAC
KspI 1083 CCGCGG CCGC^GG
2615 CCGCGG
MaeI 1364 CTAG C^TAG
2404 CTAG
MflI 411 AGATCT [GA]^GATC[CT]
2168 GGATCT
MluNI 1469 TGGCCA TGG^CCA
2438 TGGCCA
MscI 1469 TGGCCA TGG^CCA
2438 TGGCCA
NspI 672 GCATGC [GA]CATG^[CT]
1766 ACATGC
PflFI 939 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3283 GACGACGTC
SacII 1083 CCGCGG CCGC^GG
2615 CCGCGG
SfiI 2439 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3148 GGCCGGGCAGGCC
SspBI 1280 TGTACA T^GTACA
1346 TGTACA
StuI 1529 AGGCCT AGG^CCT
2930 AGGCCT
Tth111I 939 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3283 GACGACGTC
XhoII 411 AGATCT [GA]^GATC[CT]
2168 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TfiI TseI Tsp45I TspRI Van91I
XcmI XmaCI XmaI