Restriction map for
pF1KA0386
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AscI AseI AsnI
AspI AviII AvrII BbeI BbrPI
BclI BfrI BglII BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrDI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI KasI Ksp632I KspI
MaeII MluI MroI MvnI NarI
NotI NruI NsiI NspI PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 41 enzymes) #
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AhdI 3042 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 570 GTGCAC G^TGCAC
Aor51HI 2145 AGCGCT AGC^GCT
ApaI 34 GGGCCC GGGCC^C
ApaLI 570 GTGCAC G^TGCAC
Asp700I 1615 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3042 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 10 GGATCC G^GATCC
BcgI 2255 GCACCCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 256 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 853 GAGACC GGTCTC(1/5)
BsmBI 387 GAGACG CGTCTC(1/5)
Bsp1407I 1025 TGTACA T^GTACA
BsrFI 689 GCCGGC [GA]^CCGG[CT]
BsrGI 1025 TGTACA T^GTACA
BssSI 2263 CTCGTG CACGAG(-5/-1)
BstBI 2573 TTCGAA TT^CGAA
BstXI 575 CCATTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 689 GCCGGC [GA]^CCGG[CT]
Eam1105I 3042 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2145 AGCGCT AGC^GCT
EcoRI 607 GAATTC G^AATTC
FauI 67 GCGGG CCCGC(4/6)
MamI 256 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 689 GCCGGC GCC^GGC
NcoI 2732 CCATGG C^CATGG
NdeI 3125 CATATG CA^TATG
NgoMIV 689 GCCGGC G^CCGGC
NheI 816 GCTAGC G^CTAGC
NspV 2573 TTCGAA TT^CGAA
PaeR7I 3149 CTCGAG C^TCGAG
SalI 895 GTCGAC G^TCGAC
SexAI 940 ACCTGGT A^CC[AT]GGT
SfuI 2573 TTCGAA TT^CGAA
SmaI 515 CCCGGG CCC^GGG
SspBI 1025 TGTACA T^GTACA
XbaI 1797 TCTAGA T^CTAGA
XhoI 3149 CTCGAG C^TCGAG
XmaCI 515 CCCGGG C^CCGGG
XmaI 515 CCCGGG C^CCGGG
XmnI 1615 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 913 GGTACC G^GTACC
1036 GGTACC
Asp718I 913 GGTACC G^GTACC
1036 GGTACC
AvaI 515 CCCGGG C^[CT]CG[GA]G
3149 CTCGAG
BalI 1733 TGGCCA TGG^CCA
2717 TGGCCA
BbvCI 205 CCTCAGC CCTCAGC(-5/-2)
1401 CCTCAGC
BciVI 440 GTATCC GTATCC(6/5)
945 GTATCC
BglI 27 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1104 GCCCAGGCGGC
BsoBI 515 CCCGGG C^[CT]CG[GA]G
3149 CTCGAG
BspHI 677 TCATGA T^CATGA
1895 TCATGA
BsrBI 128 CCGCTC CCGCTC(-3/-3)
1167 CCGCTC
DraII 33 AGGGCCC [GA]G^G[ACGT]CC[CT]
235 AGGGCCT
DsaI 2679 CCACGG C^C[GA][CT]GG
2732 CCATGG
EcoO109I 33 AGGGCCC [GA]G^G[ACGT]CC[CT]
235 AGGGCCT
HaeII 982 GGCGCT [GA]GCGC^[CT]
2145 AGCGCT
KpnI 913 GGTACC GGTAC^C
1036 GGTACC
MfeI 371 CAATTG C^AATTG
3133 CAATTG
MluNI 1733 TGGCCA TGG^CCA
2717 TGGCCA
MscI 1733 TGGCCA TGG^CCA
2717 TGGCCA
MunI 371 CAATTG C^AATTG
3133 CAATTG
PflMI 2340 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2589 CCAAATTCTGG
RcaI 677 TCATGA T^CATGA
1895 TCATGA
ScaI 2249 AGTACT AGT^ACT
2823 AGTACT
StuI 1640 AGGCCT AGG^CCT
2930 AGGCCT
TfiI 594 GAATC G^A[AT]TC
1774 GAATC
Van91I 2340 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2589 CCAAATTCTGG
XcmI 1457 CCAGGAACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2798 CCAGCAACCTCCTGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII EaeI Eco81I EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII