Restriction map for
pF1KA0051
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AgeI AhdI Alw44I ApaI ApaLI
AscI AspEI AviII BbeI BbrPI
BglI BsaAI BsgI BsiWI Bsp1407I
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Cfr10I CpoI Eam1105I EcoO65I EcoT22I
FseI FspI HpaI KasI KspI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SspBI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 104 GACGTC GACGT^C
Acc65I 4481 GGTACC G^GTACC
AccI 226 GTCTAC GT^[AC][GT]AC
AccII 3036 CGCG CG^CG
AccIII 3951 TCCGGA T^CCGGA
AclI 4166 AACGTT AA^CGTT
AcyI 104 GACGTC G[GA]^CG[CT]C
AflII 3741 CTTAAG C^TTAAG
AflIII 3035 ACGCGT A^C[GA][CT]GT
AocI 1871 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2468 AGCGCT AGC^GCT
Asp700I 246 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4481 GGTACC G^GTACC
AspI 1313 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2586 GGATCC G^GATCC
BbvCI 2581 GCTGAGG CCTCAGC(-5/-2)
BfrI 3741 CTTAAG C^TTAAG
BlpI 793 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 793 GCTAAGC GC^T[ACGT]AGC
BsaBI 1279 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 104 GACGTC G[GA]^CG[CT]C
BseAI 3951 TCCGGA T^CCGGA
BsiEI 40 CGGCCG CG[GA][CT]^CG
BspDI 3578 ATCGAT AT^CGAT
BspEI 3951 TCCGGA T^CCGGA
BstBI 4801 TTCGAA TT^CGAA
BstUI 3036 CGCG CG^CG
Bsu36I 1871 CCTTAGG CC^T[ACGT]AGG
CelII 793 GCTAAGC GC^T[ACGT]AGC
ClaI 3578 ATCGAT AT^CGAT
DraIII 1925 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 40 CGGCCG C^GGCCG
EclXI 40 CGGCCG C^GGCCG
Eco47III 2468 AGCGCT AGC^GCT
Eco52I 40 CGGCCG C^GGCCG
Eco81I 1871 CCTTAGG CC^T[ACGT]AGG
HgaI 3037 GCGTC GACGC(5/10)
Hin1I 104 GACGTC G[GA]^CG[CT]C
KpnI 4481 GGTACC GGTAC^C
MamI 1279 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1432 CAATTG C^AATTG
MluI 3035 ACGCGT A^CGCGT
MroI 3951 TCCGGA T^CCGGA
MunI 1432 CAATTG C^AATTG
MvnI 3036 CGCG CG^CG
NcoI 365 CCATGG C^CATGG
NheI 3363 GCTAGC G^CTAGC
NspV 4801 TTCGAA TT^CGAA
PacI 1630 TTAATTAA TTAAT^TAA
PflFI 1313 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4166 AACGTT AA^CGTT
SacI 1345 GAGCTC GAGCT^C
SapI 751 GAAGAGC GCTCTTC(1/4)
SexAI 1586 ACCTGGT A^CC[AT]GGT
SfuI 4801 TTCGAA TT^CGAA
SpeI 4506 ACTAGT A^CTAGT
SspI 1462 AATATT AAT^ATT
SwaI 4040 ATTTAAAT ATTT^AAAT
Tth111I 1313 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 246 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 1345 GAGCTC G[AT]GC[AT]^C
3955 GAGCAC
AvaI 2162 CTCGGG C^[CT]CG[GA]G
2552 CTCGGG
AvrII 171 CCTAGG C^CTAGG
4642 CCTAGG
BcnI 38 CCCGG CC^[GC]GG
2496 CCGGG
BglII 180 AGATCT A^GATCT
3209 AGATCT
BlnI 171 CCTAGG C^CTAGG
4642 CCTAGG
BsaWI 3951 TCCGGA [AT]^CCGG[AT]
4187 TCCGGT
BsiHKAI 1345 GAGCTC G[AT]GC[AT]^C
3955 GAGCAC
BsmBI 102 GAGACG CGTCTC(1/5)
4275 GAGACG
BsoBI 2162 CTCGGG C^[CT]CG[GA]G
2552 CTCGGG
BspHI 3369 TCATGA T^CATGA
4895 TCATGA
BstAPI 646 GCATTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1132 GCAAACAGTGC
DrdI 1073 GACAGAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4074 GACGGAAGTGTC
FauI 2676 GCGGG CCCGC(4/6)
3414 GCGGG
HaeII 2468 AGCGCT [GA]GCGC^[CT]
3360 GGCGCT
HindIII 1565 AAGCTT A^AGCTT
2909 AAGCTT
NciI 38 CCCGG CC^[GC]GG
2496 CCGGG
PpuMI 4733 AGGACCT [GA]G^G[AT]CC[CT]
4859 AGGACCT
RcaI 3369 TCATGA T^CATGA
4895 TCATGA
ScaI 2489 AGTACT AGT^ACT
3848 AGTACT
SphI 166 GCATGC GCATG^C
3699 GCATGC
StuI 486 AGGCCT AGG^CCT
2238 AGGCCT
XcmI 191 CCACCACAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2993 CCACCAAGTTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaII
BalI BanI BanII BbsI BbvI
BcgI BciVI BclI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII