Restriction map for
pF1KSDF0380
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbsI
BbvCI BcgI BclI BfrI BglI
BlnI BpuAI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 660 CGCG CG^CG
AcsI 1414 GAATTT [GA]^AATT[CT]
AlwNI 2143 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1414 GAATTT [GA]^AATT[CT]
Asp700I 28 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 909 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1671 GGATCC G^GATCC
BbeI 1976 GGCGCC GGCGC^C
BbrPI 1708 CACGTG CAC^GTG
BsaAI 1708 CACGTG [CT]AC^GT[GA]
BsaWI 2306 ACCGGA [AT]^CCGG[AT]
BsmI 804 GAATGC GAATGC(1/-1)
Bsp1407I 1007 TGTACA T^GTACA
BsrGI 1007 TGTACA T^GTACA
BstAPI 1738 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 660 CGCG CG^CG
DsaI 974 CCATGG C^C[GA][CT]GG
HaeII 1976 GGCGCC [GA]GCGC^[CT]
HgaI 1096 GCGTC GACGC(5/10)
KasI 1976 GGCGCC G^GCGCC
MvnI 660 CGCG CG^CG
NarI 1976 GGCGCC GG^CGCC
NcoI 974 CCATGG C^CATGG
PaeR7I 370 CTCGAG C^TCGAG
PflFI 909 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 484 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1708 CACGTG CAC^GTG
PmlI 1708 CACGTG CAC^GTG
PpuMI 2060 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 577 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 1089 GCATC GCATC(5/9)
SfoI 1976 GGCGCC GGC^GCC
SspBI 1007 TGTACA T^GTACA
TaqI 371 TCGA T^CGA
Tth111I 909 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 371 TCGA T^CGA
Van91I 484 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 370 CTCGAG C^TCGAG
XmnI 28 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 1095 GGCGTC G[GA]^CG[CT]C
1976 GGCGCC
AocI 171 CCTGAGG CC^T[ACGT]AGG
768 CCTTAGG
ApaI 133 GGGCCC GGGCC^C
263 GGGCCC
BalI 1160 TGGCCA TGG^CCA
1364 TGGCCA
BciVI 84 GGATAC GTATCC(6/5)
197 GTATCC
BglII 516 AGATCT A^GATCT
945 AGATCT
BlpI 1678 GCTCAGC GC^T[ACGT]AGC
1953 GCTGAGC
Bpu1102I 1678 GCTCAGC GC^T[ACGT]AGC
1953 GCTGAGC
BsaBI 522 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
544 GATATTGATC
BsaHI 1095 GGCGTC G[GA]^CG[CT]C
1976 GGCGCC
BsgI 716 CTGCAC GTGCAG(16/14)
1390 GTGCAG
BstXI 1260 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1742 CCAGTGCCCTGG
Bsu36I 171 CCTGAGG CC^T[ACGT]AGG
768 CCTTAGG
CelII 1678 GCTCAGC GC^T[ACGT]AGC
1953 GCTGAGC
DraIII 1739 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1772 CACCCAGTG
DrdI 744 GACGGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2233 GACGGCAGTGTC
Eco81I 171 CCTGAGG CC^T[ACGT]AGG
768 CCTTAGG
EcoT14I 182 CCTTGG C^C[AT][AT]GG
974 CCATGG
FauI 2255 CCCGC CCCGC(4/6)
2091 GCGGG
Hin1I 1095 GGCGTC G[GA]^CG[CT]C
1976 GGCGCC
MaeII 80 ACGT A^CGT
1709 ACGT
MamI 522 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
544 GATATTGATC
MluNI 1160 TGGCCA TGG^CCA
1364 TGGCCA
MscI 1160 TGGCCA TGG^CCA
1364 TGGCCA
MslI 960 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
975 CATGGAAGTG
MspA1I 1402 CAGCTG C[AC]G^C[GT]G
1951 CAGCTG
PstI 1110 CTGCAG CTGCA^G
2065 CTGCAG
PvuII 1402 CAGCTG CAG^CTG
1951 CAGCTG
SapI 871 GCTCTTC GCTCTTC(1/4)
1245 GCTCTTC
SmlI 370 CTCGAG C^T[CT][GA]AG
1783 CTCAAG
StyI 182 CCTTGG C^C[AT][AT]GG
974 CCATGG
TfiI 443 GATTC G^A[AT]TC
2040 GAATC
XcmI 2033 CCATCCTGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2154 CCAGGAGGTGTTTGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfcI StuI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII