Restriction map for
pF1KSDF0258
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AclI AflIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbeI BbrPI BglI BglII BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 1233 GACGTC GACGT^C
AccII 327 CGCG CG^CG
AccIII 1548 TCCGGA T^CCGGA
AcsI 223 GAATTT [GA]^AATT[CT]
AcyI 1233 GACGTC G[GA]^CG[CT]C
AflII 523 CTTAAG C^TTAAG
AocI 367 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2084 AGCGCT AGC^GCT
ApoI 223 GAATTT [GA]^AATT[CT]
Asp700I 1954 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1448 TGCGCA TGC^GCA
BamHI 1442 GGATCC G^GATCC
BanI 1310 GGTGCC G^G[CT][GA]CC
BcgI 1845 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 795 GGATAC GTATCC(6/5)
BclI 1107 TGATCA T^GATCA
BfaI 301 CTAG C^TAG
BfrI 523 CTTAAG C^TTAAG
BlpI 51 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 51 GCTCAGC GC^T[ACGT]AGC
BsaHI 1233 GACGTC G[GA]^CG[CT]C
BsaWI 1548 TCCGGA [AT]^CCGG[AT]
BseAI 1548 TCCGGA T^CCGGA
BsgI 1558 CTGCAC GTGCAG(16/14)
BsiEI 329 CGGCCG CG[GA][CT]^CG
BsmBI 1900 GAGACG CGTCTC(1/5)
BsmI 658 GAATGC GAATGC(1/-1)
BspEI 1548 TCCGGA T^CCGGA
BsrBI 346 CCGCTC CCGCTC(-3/-3)
BsrDI 550 GCAATG GCAATG(2/0)
BstUI 327 CGCG CG^CG
Bsu36I 367 CCTGAGG CC^T[ACGT]AGG
CelII 51 GCTCAGC GC^T[ACGT]AGC
DraIII 646 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1777 GACCCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 329 CGGCCG C^GGCCG
EclXI 329 CGGCCG C^GGCCG
Eco47III 2084 AGCGCT AGC^GCT
Eco52I 329 CGGCCG C^GGCCG
Eco81I 367 CCTGAGG CC^T[ACGT]AGG
FbaI 1107 TGATCA T^GATCA
FspI 1448 TGCGCA TGC^GCA
Hin1I 1233 GACGTC G[GA]^CG[CT]C
HincII 2203 GTCAAC GT[CT]^[GA]AC
HindII 2203 GTCAAC GT[CT]^[GA]AC
MaeI 301 CTAG C^TAG
MroI 1548 TCCGGA T^CCGGA
MslI 810 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 327 CGCG CG^CG
NaeI 830 GCCGGC GCC^GGC
NgoMIV 830 GCCGGC G^CCGGC
NheI 300 GCTAGC G^CTAGC
NspI 254 ACATGC [GA]CATG^[CT]
PshAI 1495 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 647 ACCTGGT A^CC[AT]GGT
SmaI 1407 CCCGGG CCC^GGG
SmlI 523 CTTAAG C^T[CT][GA]AG
StuI 635 AGGCCT AGG^CCT
TaqI 457 TCGA T^CGA
TthHB8I 457 TCGA T^CGA
XmaCI 1407 CCCGGG C^CCGGG
XmaI 1407 CCCGGG C^CCGGG
XmnI 1954 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 37 GAGCTC G[AT]GC[AT]^C
528 GAGCTC
BalI 1466 TGGCCA TGG^CCA
2006 TGGCCA
BbsI 1209 GAAGAC GAAGAC(2/6)
2298 GAAGAC
BpuAI 1209 GAAGAC GAAGAC(2/6)
2298 GAAGAC
BsaAI 814 TACGTG [CT]AC^GT[GA]
1570 TACGTG
BsiHKAI 37 GAGCTC G[AT]GC[AT]^C
528 GAGCTC
BsrFI 830 GCCGGC [GA]^CCGG[CT]
1454 ACCGGC
BssSI 67 CACGAG CACGAG(-5/-1)
844 CACGAG
BstAPI 123 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
843 GCACGAGCTGC
BstXI 197 CCAGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
641 CCATGCACCTGG
Cfr10I 830 GCCGGC [GA]^CCGG[CT]
1454 ACCGGC
DsaI 107 CCGTGG C^C[GA][CT]GG
2003 CCGTGG
MluNI 1466 TGGCCA TGG^CCA
2006 TGGCCA
MscI 1466 TGGCCA TGG^CCA
2006 TGGCCA
MspA1I 25 CAGCTG C[AC]G^C[GT]G
715 CAGCTG
PpuMI 399 GGGACCC [GA]G^G[AT]CC[CT]
974 AGGTCCC
PvuII 25 CAGCTG CAG^CTG
715 CAGCTG
SacI 37 GAGCTC GAGCT^C
528 GAGCTC
SapI 597 GAAGAGC GCTCTTC(1/4)
1125 GAAGAGC
Tsp509I 224 AATT ^AATT
1982 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BanII BbvCI
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I TspRI Van91I XhoII