Restriction map for
pF1KSDF0166
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TseI Tth111I
Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 962 GACGCC G[GA]^CG[CT]C
AflII 498 CTTAAG C^TTAAG
ApaI 129 GGGCCC GGGCC^C
AseI 268 ATTAAT AT^TAAT
AsnI 268 ATTAAT AT^TAAT
AvaI 125 CCCGGG C^[CT]CG[GA]G
BamHI 531 GGATCC G^GATCC
BanII 129 GGGCCC G[GA]GC[CT]^C
BbsI 274 GAAGAC GAAGAC(2/6)
BfrI 498 CTTAAG C^TTAAG
BmyI 129 GGGCCC G[AGT]GC[ACT]^C
BpuAI 274 GAAGAC GAAGAC(2/6)
BsaHI 962 GACGCC G[GA]^CG[CT]C
BseRI 1163 GAGGAG GAGGAG(10/8)
BsiEI 110 CGGCCG CG[GA][CT]^CG
BsmAI 1237 GAGAC GTCTC(1/5)
BsoBI 125 CCCGGG C^[CT]CG[GA]G
Bsp1286I 129 GGGCCC G[AGT]GC[ACT]^C
BspHI 455 TCATGA T^CATGA
BsrI 553 ACTGG ACTGG(1/-1)
BssSI 1037 CTCGTG CACGAG(-5/-1)
EaeI 110 CGGCCG [CT]^GGCC[GA]
EagI 110 CGGCCG C^GGCCG
EclXI 110 CGGCCG C^GGCCG
Eco52I 110 CGGCCG C^GGCCG
FauI 132 CCCGC CCCGC(4/6)
HgaI 962 GACGC GACGC(5/10)
Hin1I 962 GACGCC G[GA]^CG[CT]C
MaeII 1019 ACGT A^CGT
NcoI 525 CCATGG C^CATGG
NheI 606 GCTAGC G^CTAGC
PleI 733 GAGTC GAGTC(4/5)
PpuMI 8 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 268 ATTAAT AT^TAAT
RcaI 455 TCATGA T^CATGA
SfcI 696 CTACAG C^T[GA][CT]AG
SmaI 125 CCCGGG CCC^GGG
SmlI 498 CTTAAG C^T[CT][GA]AG
StuI 846 AGGCCT AGG^CCT
Tsp45I 557 GTGAC ^GT[GC]AC
TspRI 279 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 798 TCTAGA T^CTAGA
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 3 GGACC G^G[AT]CC
9 GGACC
BsmFI 8 GGGAC GGGAC(10/14)
494 GTCCC
BspMI 252 ACCTGC ACCTGC(4/8)
1287 GCAGGT
BstYI 216 GGATCT [GA]^GATC[CT]
531 GGATCC
DdeI 474 CTTAG C^T[ACGT]AG
1308 CTTAG
DraII 8 GGGACCT [GA]G^G[ACGT]CC[CT]
128 GGGGCCC
DsaI 36 CCACGG C^C[GA][CT]GG
525 CCATGG
EcoO109I 8 GGGACCT [GA]G^G[ACGT]CC[CT]
128 GGGGCCC
EcoRI 412 GAATTC G^AATTC
790 GAATTC
EcoT14I 525 CCATGG C^C[AT][AT]GG
761 CCTTGG
HapII 6 CCGG C^CGG
126 CCGG
HincII 175 GTTGAC GT[CT]^[GA]AC
943 GTTGAC
HindII 175 GTTGAC GT[CT]^[GA]AC
943 GTTGAC
HpaII 6 CCGG C^CGG
126 CCGG
MflI 216 GGATCT [GA]^GATC[CT]
531 GGATCC
MslI 967 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1120 CACAGAAATG
MspI 6 CCGG C^CGG
126 CCGG
SapI 679 GAAGAGC GCTCTTC(1/4)
626 GCTCTTC
StyI 525 CCATGG C^C[AT][AT]GG
761 CCTTGG
TaqI 185 TCGA T^CGA
567 TCGA
TthHB8I 185 TCGA T^CGA
567 TCGA
XhoII 216 GGATCT [GA]^GATC[CT]
531 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I BcnI BfaI BsaJI BsiYI
BslI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I Tsp509I XmnI