Restriction map for
pF1KSDF0136
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccI AclI AcsI AflIII
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BamHI BbrPI BbvCI BciVI BclI
BfaI BglII BlnI BlpI Bpu1102I
BsaBI BsiEI BsiWI BsmBI BsmI
BspDI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MaeI MamI
MfeI MflI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI StyI
SwaI TfiI Tsp509I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 2147 GGTACC G^GTACC
AccIII 2051 TCCGGA T^CCGGA
AflII 507 CTTAAG C^TTAAG
AlwI 1377 GATCC GGATC(4/5)
AocI 817 CCTCAGG CC^T[ACGT]AGG
Asp718I 2147 GGTACC G^GTACC
AspI 1666 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2367 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 507 CTTAAG C^TTAAG
BsaAI 1519 TACGTG [CT]AC^GT[GA]
BseAI 2051 TCCGGA T^CCGGA
Bsp1407I 1076 TGTACA T^GTACA
BspEI 2051 TCCGGA T^CCGGA
BspHI 1373 TCATGA T^CATGA
BsrGI 1076 TGTACA T^GTACA
Bsu36I 817 CCTCAGG CC^T[ACGT]AGG
Eco81I 817 CCTCAGG CC^T[ACGT]AGG
HindIII 2308 AAGCTT A^AGCTT
KpnI 2147 GGTACC GGTAC^C
MroI 2051 TCCGGA T^CCGGA
PaeR7I 2031 CTCGAG C^TCGAG
PflFI 1666 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 547 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1246 CAGCTG CAG^CTG
RcaI 1373 TCATGA T^CATGA
ScaI 299 AGTACT AGT^ACT
SphI 251 GCATGC GCATG^C
SspBI 1076 TGTACA T^GTACA
SspI 1207 AATATT AAT^ATT
StuI 867 AGGCCT AGG^CCT
Tth111I 1666 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2031 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AgeI 1649 ACCGGT A^CCGGT
2144 ACCGGT
BbeI 1911 GGCGCC GGCGC^C
1987 GGCGCC
BsaI 37 GAGACC GGTCTC(1/5)
2121 GGTCTC
BsmAI 37 GAGAC GTCTC(1/5)
2122 GTCTC
BsrBI 363 CCGCTC CCGCTC(-3/-3)
166 GAGCGG
BsrDI 1764 CATTGC GCAATG(2/0)
2273 GCAATG
BstAPI 974 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2380 GCACCCCCTGC
HgaI 1173 GACGC GACGC(5/10)
1601 GCGTC
KasI 1911 GGCGCC G^GCGCC
1987 GGCGCC
MaeII 1358 ACGT A^CGT
1520 ACGT
MseI 508 TTAA T^TAA
2299 TTAA
NarI 1911 GGCGCC GG^CGCC
1987 GGCGCC
PinAI 1649 ACCGGT A^CCGGT
2144 ACCGGT
SacI 454 GAGCTC GAGCT^C
874 GAGCTC
SapI 540 GAAGAGC GCTCTTC(1/4)
1821 GCTCTTC
SbfI 669 CCTGCAGG CCTGCA^GG
1605 CCTGCAGG
SfoI 1911 GGCGCC GGC^GCC
1987 GGCGCC
Sse8387I 669 CCTGCAGG CCTGCA^GG
1605 CCTGCAGG
TaqI 26 TCGA T^CGA
2032 TCGA
Tru9I 508 TTAA T^TAA
2299 TTAA
Tsp45I 175 GTGAC ^GT[GC]AC
1171 GTGAC
TthHB8I 26 TCGA T^CGA
2032 TCGA
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI TspRI Van91I