Restriction map for
pF1KSDF0044
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# NO CUT ENZYMES ( 86 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbsI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I EcoRV EcoT22I
FbaI HindIII HpaI KpnI MaeI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SfiI SfuI
SmlI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 1105 GACGTC GACGT^C
AcsI 2830 GAATTC [GA]^AATT[CT]
AocI 2684 CCTTAGG CC^T[ACGT]AGG
Aor51HI 659 AGCGCT AGC^GCT
ApoI 2830 GAATTC [GA]^AATT[CT]
BalI 1806 TGGCCA TGG^CCA
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
BsaBI 2124 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 568 GAGACG CGTCTC(1/5)
BssHII 898 GCGCGC G^CGCGC
BssSI 2578 CACGAG CACGAG(-5/-1)
BstEII 256 GGTTACC G^GT[ACGT]ACC
BstPI 256 GGTTACC G^GT[ACGT]ACC
Bsu36I 2684 CCTTAGG CC^T[ACGT]AGG
DrdI 2749 GACGCGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 659 AGCGCT AGC^GCT
Eco81I 2684 CCTTAGG CC^T[ACGT]AGG
EcoO65I 256 GGTTACC G^GT[ACGT]ACC
EcoRI 2830 GAATTC G^AATTC
FseI 88 GGCCGGCC GGCCGG^CC
MamI 2124 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1806 TGGCCA TGG^CCA
MscI 1806 TGGCCA TGG^CCA
MseI 1934 TTAA T^TAA
NcoI 1163 CCATGG C^CATGG
NspI 902 GCATGC [GA]CATG^[CT]
SbfI 930 CCTGCAGG CCTGCA^GG
SexAI 539 ACCAGGT A^CC[AT]GGT
SgrAI 729 CGCCGGCG C[GA]^CCGG[CT]G
SphI 902 GCATGC GCATG^C
Sse8387I 930 CCTGCAGG CCTGCA^GG
StuI 1277 AGGCCT AGG^CCT
Tru9I 1934 TTAA T^TAA
Tsp509I 2831 AATT ^AATT
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 675 GATCC GGATC(4/5)
780 GATCC
AviII 1475 TGCGCA TGC^GCA
1544 TGCGCA
BbrPI 499 CACGTG CAC^GTG
1354 CACGTG
BcgI 276 CGAGCACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2580 CGAGCTCTCTGC
BlpI 655 GCTCAGC GC^T[ACGT]AGC
2493 GCTCAGC
Bpu1102I 655 GCTCAGC GC^T[ACGT]AGC
2493 GCTCAGC
BsaAI 499 CACGTG [CT]AC^GT[GA]
1354 CACGTG
BsmI 878 GAATGC GAATGC(1/-1)
524 GCATTC
BstYI 674 AGATCC [GA]^GATC[CT]
779 AGATCC
CelII 655 GCTCAGC GC^T[ACGT]AGC
2493 GCTCAGC
EagI 1853 CGGCCG C^GGCCG
2387 CGGCCG
EclXI 1853 CGGCCG C^GGCCG
2387 CGGCCG
Eco52I 1853 CGGCCG C^GGCCG
2387 CGGCCG
FspI 1475 TGCGCA TGC^GCA
1544 TGCGCA
HincII 1848 GTTGAC GT[CT]^[GA]AC
2530 GTCAAC
HindII 1848 GTTGAC GT[CT]^[GA]AC
2530 GTCAAC
MflI 674 AGATCC [GA]^GATC[CT]
779 AGATCC
PmaCI 499 CACGTG CAC^GTG
1354 CACGTG
PmlI 499 CACGTG CAC^GTG
1354 CACGTG
SacI 2248 GAGCTC GAGCT^C
2581 GAGCTC
XhoII 674 AGATCC [GA]^GATC[CT]
779 AGATCC
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XmaCI XmaI