Restriction map for
pF1KSDB1467
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AvrII BbrPI BclI BfrI
BlnI BsaWI BseAI BsmBI BspDI
BspEI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI DraI DraIII Eco47III
EcoRI FbaI FseI HpaI KpnI
KspI MluI MroI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 244 CGCG CG^CG
AflIII 3623 ACATGT A^C[GA][CT]GT
AhdI 3612 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2306 GTGCAC G^TGCAC
ApaI 4490 GGGCCC GGGCC^C
ApaLI 2306 GTGCAC G^TGCAC
AspEI 3612 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 659 GGCGCC GGCGC^C
BbvCI 1971 CCTCAGC CCTCAGC(-5/-2)
BsaAI 3561 CACGTA [CT]AC^GT[GA]
BsaBI 3291 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 3563 CGTACG C^GTACG
Bsp1407I 4409 TGTACA T^GTACA
BspLU11I 3623 ACATGT A^CATGT
BsrDI 17 GCAATG GCAATG(2/0)
BsrGI 4409 TGTACA T^GTACA
BstUI 244 CGCG CG^CG
CpoI 223 CGGACCG CG^G[AT]CCG
DrdI 1588 GACAGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3612 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1030 GATATC GAT^ATC
EcoT22I 4570 ATGCAT ATGCA^T
KasI 659 GGCGCC G^GCGCC
MamI 3291 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 589 CAATTG C^AATTG
MunI 589 CAATTG C^AATTG
MvnI 244 CGCG CG^CG
NarI 659 GGCGCC GG^CGCC
NcoI 1977 CCATGG C^CATGG
NheI 69 GCTAGC G^CTAGC
NsiI 4570 ATGCAT ATGCA^T
RsrII 223 CGGACCG CG^G[AT]CCG
SfiI 3000 GGCCAAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 659 GGCGCC GGC^GCC
SspBI 4409 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 193 GTAGAC GT^[AC][GT]AC
1201 GTAGAC
AcsI 1703 AAATTC [GA]^AATT[CT]
4628 AAATTT
AcyI 659 GGCGCC G[GA]^CG[CT]C
897 GACGCC
ApoI 1703 AAATTC [GA]^AATT[CT]
4628 AAATTT
Asp700I 3078 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4128 GAAGATCTTC
AspI 1855 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2329 GACAGTGTC
AviII 1400 TGCGCA TGC^GCA
3335 TGCGCA
BcgI 2527 CGATATTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3285 GCAGATGATTCG
BciVI 498 GGATAC GTATCC(6/5)
3918 GGATAC
BlpI 2737 GCTGAGC GC^T[ACGT]AGC
4767 GCTGAGC
Bpu1102I 2737 GCTGAGC GC^T[ACGT]AGC
4767 GCTGAGC
BsaHI 659 GGCGCC G[GA]^CG[CT]C
897 GACGCC
BsiEI 3366 CGGCCG CG[GA][CT]^CG
4302 CGGCCG
BsmI 891 GAATGC GAATGC(1/-1)
2785 GAATGC
BspHI 1329 TCATGA T^CATGA
3299 TCATGA
BsrBI 4305 CCGCTC CCGCTC(-3/-3)
4330 GAGCGG
BssSI 4189 CACGAG CACGAG(-5/-1)
3102 CTCGTG
BstEII 3438 GGTGACC G^GT[ACGT]ACC
4741 GGTGACC
BstPI 3438 GGTGACC G^GT[ACGT]ACC
4741 GGTGACC
CelII 2737 GCTGAGC GC^T[ACGT]AGC
4767 GCTGAGC
EagI 3366 CGGCCG C^GGCCG
4302 CGGCCG
EclXI 3366 CGGCCG C^GGCCG
4302 CGGCCG
Eco52I 3366 CGGCCG C^GGCCG
4302 CGGCCG
EcoO65I 3438 GGTGACC G^GT[ACGT]ACC
4741 GGTGACC
FspI 1400 TGCGCA TGC^GCA
3335 TGCGCA
HgaI 897 GACGC GACGC(5/10)
2697 GCGTC
Hin1I 659 GGCGCC G[GA]^CG[CT]C
897 GACGCC
HincII 1126 GTCAAC GT[CT]^[GA]AC
3772 GTTGAC
HindII 1126 GTCAAC GT[CT]^[GA]AC
3772 GTTGAC
HindIII 2254 AAGCTT A^AGCTT
2452 AAGCTT
PflFI 1855 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2329 GACAGTGTC
RcaI 1329 TCATGA T^CATGA
3299 TCATGA
SapI 2015 GAAGAGC GCTCTTC(1/4)
4254 GCTCTTC
SexAI 1784 ACCTGGT A^CC[AT]GGT
1856 ACCTGGT
Tth111I 1855 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2329 GACAGTGTC
XmnI 3078 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4128 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI