Restriction map for
pF1KSDB0185
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI ApaI AseI AsnI Asp718I
AspI AvrII BciVI BfaI BfrI
BlnI BsiWI Bsp1407I BspDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EcoRI EcoRV EcoT22I
FseI HindIII HpaI KpnI KspI
MaeI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcsI 1613 AAATTT [GA]^AATT[CT]
AhdI 4441 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4566 GTGCAC G^TGCAC
Aor51HI 2191 AGCGCT AGC^GCT
ApaLI 4566 GTGCAC G^TGCAC
ApoI 1613 AAATTT [GA]^AATT[CT]
AscI 2414 GGCGCGCC GG^CGCGCC
AspEI 4441 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 110 GGATCC G^GATCC
BbeI 4475 GGCGCC GGCGC^C
BclI 305 TGATCA T^GATCA
BglII 4871 AGATCT A^GATCT
BsmBI 3631 GAGACG CGTCTC(1/5)
BsmI 3151 GCATTC GAATGC(1/-1)
BspHI 965 TCATGA T^CATGA
BspLU11I 488 ACATGT A^CATGT
BssHII 2415 GCGCGC G^CGCGC
DraIII 2161 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4441 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2191 AGCGCT AGC^GCT
FbaI 305 TGATCA T^GATCA
KasI 4475 GGCGCC G^GCGCC
NaeI 2092 GCCGGC GCC^GGC
NarI 4475 GGCGCC GG^CGCC
NgoMIV 2092 GCCGGC G^CCGGC
PshAI 2628 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 965 TCATGA T^CATGA
SbfI 3075 CCTGCAGG CCTGCA^GG
ScaI 1376 AGTACT AGT^ACT
SfoI 4475 GGCGCC GGC^GCC
SphI 645 GCATGC GCATG^C
Sse8387I 3075 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1637 TCCGGA T^CCGGA
3842 TCCGGA
AcyI 1912 GACGCC G[GA]^CG[CT]C
4475 GGCGCC
AflIII 488 ACATGT A^C[GA][CT]GT
3566 ACGTGT
AocI 3761 CCTCAGG CC^T[ACGT]AGG
3892 CCTGAGG
BsaBI 111 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1413 GATCAAGATC
BsaHI 1912 GACGCC G[GA]^CG[CT]C
4475 GGCGCC
BsaWI 1637 TCCGGA [AT]^CCGG[AT]
3842 TCCGGA
BseAI 1637 TCCGGA T^CCGGA
3842 TCCGGA
BspEI 1637 TCCGGA T^CCGGA
3842 TCCGGA
BsrBI 1821 GAGCGG CCGCTC(-3/-3)
2887 GAGCGG
Bsu36I 3761 CCTCAGG CC^T[ACGT]AGG
3892 CCTGAGG
EagI 1466 CGGCCG C^GGCCG
3735 CGGCCG
EclXI 1466 CGGCCG C^GGCCG
3735 CGGCCG
Eco52I 1466 CGGCCG C^GGCCG
3735 CGGCCG
Eco81I 3761 CCTCAGG CC^T[ACGT]AGG
3892 CCTGAGG
Hin1I 1912 GACGCC G[GA]^CG[CT]C
4475 GGCGCC
HincII 1147 GTCAAC GT[CT]^[GA]AC
2938 GTCAAC
HindII 1147 GTCAAC GT[CT]^[GA]AC
2938 GTCAAC
MamI 111 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1413 GATCAAGATC
MroI 1637 TCCGGA T^CCGGA
3842 TCCGGA
SacI 2671 GAGCTC GAGCT^C
3979 GAGCTC
SexAI 1225 ACCTGGT A^CC[AT]GGT
1400 ACCTGGT
SmlI 2074 CTCAAG C^T[CT][GA]AG
4627 CTCAAG
StuI 3320 AGGCCT AGG^CCT
4709 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaI AvaII
AviII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcgI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI