Restriction map for
pF1KSDB0041
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 1312 CGCG CG^CG
AcsI 871 AAATTT [GA]^AATT[CT]
AhdI 150 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 626 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 871 AAATTT [GA]^AATT[CT]
AspEI 150 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 139 GAAGAC GAAGAC(2/6)
BbvCI 605 CCTCAGC CCTCAGC(-5/-2)
BcnI 1341 CCGGG CC^[GC]GG
BfaI 292 CTAG C^TAG
BglII 511 AGATCT A^GATCT
BpuAI 139 GAAGAC GAAGAC(2/6)
BsaI 1143 GAGACC GGTCTC(1/5)
BsgI 595 GTGCAG GTGCAG(16/14)
BspMI 618 ACCTGC ACCTGC(4/8)
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrI 315 CCAGT ACTGG(1/-1)
BstUI 1312 CGCG CG^CG
EaeI 1212 TGGCCG [CT]^GGCC[GA]
Eam1105I 150 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 922 GAAGAG CTCTTC(1/4)
FauI 5 GCGGG CCCGC(4/6)
HapII 1341 CCGG C^CGG
HpaII 1341 CCGG C^CGG
HphI 1000 GGTGA GGTGA(8/7)
Ksp632I 922 GAAGAG CTCTTC(1/4)
MaeI 292 CTAG C^TAG
MaeIII 209 GTGAC ^GT[ACGT]AC
MslI 823 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 629 CAGCTG C[AC]G^C[GT]G
MspI 1341 CCGG C^CGG
MvnI 1312 CGCG CG^CG
NciI 1341 CCGGG CC^[GC]GG
PvuII 629 CAGCTG CAG^CTG
SspI 1114 AATATT AAT^ATT
StuI 661 AGGCCT AGG^CCT
Tsp45I 209 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 19 enzymes) #
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Acc65I 460 GGTACC G^GTACC
1285 GGTACC
Asp718I 460 GGTACC G^GTACC
1285 GGTACC
AspHI 703 GAGCAC G[AT]GC[AT]^C
788 GTGCTC
AvaI 170 CTCGAG C^[CT]CG[GA]G
1301 CTCGAG
BciVI 504 GGATAC GTATCC(6/5)
1222 GGATAC
BpmI 623 CTCCAG CTGGAG(16/14)
782 CTCCAG
BseRI 1235 GAGGAG GAGGAG(10/8)
1325 GAGGAG
BsiHKAI 703 GAGCAC G[AT]GC[AT]^C
788 GTGCTC
BsoBI 170 CTCGAG C^[CT]CG[GA]G
1301 CTCGAG
Cac8I 676 GCCAGC GC[ACGT]^[ACGT]GC
711 GCGAGC
KpnI 460 GGTACC GGTAC^C
1285 GGTACC
NcoI 320 CCATGG C^CATGG
581 CCATGG
NlaIII 321 CATG CATG^
582 CATG
PaeR7I 170 CTCGAG C^TCGAG
1301 CTCGAG
PflMI 315 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1204 CCAAACCGTGG
PleI 1015 GACTC GAGTC(4/5)
645 GAGTC
SfaNI 348 GATGC GCATC(5/9)
71 GCATC
Van91I 315 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1204 CCAAACCGTGG
XhoI 170 CTCGAG C^TCGAG
1301 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII