Restriction map for
pF1KSDB0024
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII AccII AccIII AflII AgeI
Alw44I Aor51HI ApaLI AscI AspI
AviII AvrII BbeI BbrPI BcgI
BclI BcnI BfrI BlnI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KspI MluI MroI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI SspBI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 2101 GGTACC G^GTACC
AccI 3730 GTCTAC GT^[AC][GT]AC
AcyI 3718 GGCGTC G[GA]^CG[CT]C
AflIII 3311 ACGTGT A^C[GA][CT]GT
AlwNI 3034 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 491 CCTCAGG CC^T[ACGT]AGG
ApaI 3011 GGGCCC GGGCC^C
AseI 25 ATTAAT AT^TAAT
AsnI 25 ATTAAT AT^TAAT
Asp718I 2101 GGTACC G^GTACC
BalI 2138 TGGCCA TGG^CCA
BbvCI 1652 CCTCAGC CCTCAGC(-5/-2)
BglI 2066 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3446 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3446 GCTTAGC GC^T[ACGT]AGC
BsaAI 2749 TACGTG [CT]AC^GT[GA]
BsaBI 3052 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3718 GGCGTC G[GA]^CG[CT]C
BsaI 151 GAGACC GGTCTC(1/5)
BsrBI 3393 GAGCGG CCGCTC(-3/-3)
BsrDI 3326 GCAATG GCAATG(2/0)
BstEII 3098 GGTTACC G^GT[ACGT]ACC
BstPI 3098 GGTTACC G^GT[ACGT]ACC
Bsu36I 491 CCTCAGG CC^T[ACGT]AGG
CelII 3446 GCTTAGC GC^T[ACGT]AGC
DraI 3186 TTTAAA TTT^AAA
DsaI 986 CCATGG C^C[GA][CT]GG
EaeI 2138 TGGCCA [CT]^GGCC[GA]
Eco81I 491 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3098 GGTTACC G^GT[ACGT]ACC
EcoRV 250 GATATC GAT^ATC
Hin1I 3718 GGCGTC G[GA]^CG[CT]C
HpaI 3672 GTTAAC GTT^AAC
KpnI 2101 GGTACC GGTAC^C
MamI 3052 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2138 TGGCCA TGG^CCA
MscI 2138 TGGCCA TGG^CCA
NcoI 986 CCATGG C^CATGG
PflMI 2874 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 25 ATTAAT AT^TAAT
SbfI 335 CCTGCAGG CCTGCA^GG
ScaI 1821 AGTACT AGT^ACT
SpeI 799 ACTAGT A^CTAGT
Sse8387I 335 CCTGCAGG CCTGCA^GG
SspI 1285 AATATT AAT^ATT
Van91I 2874 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2817 CCAGAGAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AclI 866 AACGTT AA^CGTT
2935 AACGTT
AcsI 304 GAATTT [GA]^AATT[CT]
2079 GAATTT
AhdI 743 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1690 GACAGCCAGTC
ApoI 304 GAATTT [GA]^AATT[CT]
2079 GAATTT
AspEI 743 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1690 GACAGCCAGTC
AspHI 544 GAGCAC G[AT]GC[AT]^C
1046 GTGCTC
AvaI 887 CCCGAG C^[CT]CG[GA]G
2425 CTCGGG
BamHI 703 GGATCC G^GATCC
1678 GGATCC
BanI 2101 GGTACC G^G[CT][GA]CC
2782 GGTGCC
BanII 2339 GAGCCC G[GA]GC[CT]^C
3011 GGGCCC
BglII 1576 AGATCT A^GATCT
3171 AGATCT
BsaWI 300 ACCGGA [AT]^CCGG[AT]
3021 TCCGGT
BsgI 311 CTGCAC GTGCAG(16/14)
608 CTGCAC
BsiHKAI 544 GAGCAC G[AT]GC[AT]^C
1046 GTGCTC
BsmI 2735 GAATGC GAATGC(1/-1)
3127 GAATGC
BsoBI 887 CCCGAG C^[CT]CG[GA]G
2425 CTCGGG
BssSI 1792 CACGAG CACGAG(-5/-1)
3608 CTCGTG
DraII 55 GGGTCCC [GA]G^G[ACGT]CC[CT]
3640 GGGTCCT
Eam1105I 743 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1690 GACAGCCAGTC
EcoO109I 55 GGGTCCC [GA]G^G[ACGT]CC[CT]
3640 GGGTCCT
HapII 301 CCGG C^CGG
3022 CCGG
HgaI 1941 GACGC GACGC(5/10)
3719 GCGTC
HincII 1316 GTCAAC GT[CT]^[GA]AC
3672 GTTAAC
HindII 1316 GTCAAC GT[CT]^[GA]AC
3672 GTTAAC
HpaII 301 CCGG C^CGG
3022 CCGG
MspI 301 CCGG C^CGG
3022 CCGG
NheI 1170 GCTAGC G^CTAGC
1944 GCTAGC
PpuMI 55 GGGTCCC [GA]G^G[AT]CC[CT]
3640 GGGTCCT
Psp1406I 866 AACGTT AA^CGTT
2935 AACGTT
PstI 336 CTGCAG CTGCA^G
2570 CTGCAG
StuI 554 AGGCCT AGG^CCT
2073 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AvaII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI