Restriction map for
pF1KA1962
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MluI MluNI MroI MscI
MspA1I MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 967 GTCTAC GT^[AC][GT]AC
AflIII 1654 ACATGT A^C[GA][CT]GT
BanI 752 GGTGCC G^G[CT][GA]CC
BanII 223 GAGCTC G[GA]GC[CT]^C
BbsI 2175 GAAGAC GAAGAC(2/6)
BclI 768 TGATCA T^GATCA
BcnI 1784 CCCGG CC^[GC]GG
BpuAI 2175 GAAGAC GAAGAC(2/6)
BsaBI 763 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2140 ACCGGA [AT]^CCGG[AT]
BspLU11I 1654 ACATGT A^CATGT
BstEII 369 GGTGACC G^GT[ACGT]ACC
BstPI 369 GGTGACC G^GT[ACGT]ACC
BstYI 411 AGATCC [GA]^GATC[CT]
Cac8I 3 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 1352 GCGC GCG^C
DraI 2182 TTTAAA TTT^AAA
DsaI 682 CCATGG C^C[GA][CT]GG
EcoO65I 369 GGTGACC G^GT[ACGT]ACC
EcoT14I 682 CCATGG C^C[AT][AT]GG
FbaI 768 TGATCA T^GATCA
HaeII 1351 AGCGCC [GA]GCGC^[CT]
HhaI 1352 GCGC GCG^C
HinP1I 1352 GCGC G^CGC
HincII 212 GTCAAC GT[CT]^[GA]AC
HindII 212 GTCAAC GT[CT]^[GA]AC
HindIII 1421 AAGCTT A^AGCTT
MamI 763 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1662 CAATTG C^AATTG
MflI 411 AGATCC [GA]^GATC[CT]
MunI 1662 CAATTG C^AATTG
NciI 1784 CCCGG CC^[GC]GG
NcoI 682 CCATGG C^CATGG
NspI 1654 ACATGT [GA]CATG^[CT]
PacI 949 TTAATTAA TTAAT^TAA
PstI 2225 CTGCAG CTGCA^G
SacI 223 GAGCTC GAGCT^C
SspI 797 AATATT AAT^ATT
StuI 2196 AGGCCT AGG^CCT
StyI 682 CCATGG C^C[AT][AT]GG
XcmI 792 CCAGCAATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 411 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 412 GATCC GGATC(4/5)
819 GGATC
AlwNI 235 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
295 CAGTTCCTG
AocI 606 CCTCAGG CC^T[ACGT]AGG
756 CCTCAGG
BsaI 243 GAGACC GGTCTC(1/5)
1587 GAGACC
BsiYI 84 CCCAAGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1364 CCAAACATCGG
BslI 84 CCCAAGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1364 CCAAACATCGG
BstNI 197 CCAGG CC^[AT]GG
313 CCTGG
Bsu36I 606 CCTCAGG CC^T[ACGT]AGG
756 CCTCAGG
DraII 80 AGGTCCC [GA]G^G[ACGT]CC[CT]
1162 AGGTCCC
EarI 1110 CTCTTC CTCTTC(1/4)
848 GAAGAG
Eco81I 606 CCTCAGG CC^T[ACGT]AGG
756 CCTCAGG
EcoO109I 80 AGGTCCC [GA]G^G[ACGT]CC[CT]
1162 AGGTCCC
EcoRII 197 CCAGG ^CC[AT]GG
313 CCTGG
HaeIII 60 GGCC GG^CC
2197 GGCC
Ksp632I 1110 CTCTTC CTCTTC(1/4)
848 GAAGAG
MaeII 1137 ACGT A^CGT
1858 ACGT
MslI 1817 CATATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1969 CATACTGGTG
MvaI 197 CCAGG CC^[AT]GG
313 CCTGG
MwoI 133 GCTGCTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
681 GCCATGGCTGC
PpuMI 80 AGGTCCC [GA]G^G[AT]CC[CT]
1162 AGGTCCC
SmlI 422 CTCAAG C^T[CT][GA]AG
972 CTTGAG
TaqI 64 TCGA T^CGA
2103 TCGA
TthHB8I 64 TCGA T^CGA
2103 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AspHI AvaII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
Cfr13I DdeI DpnII EcoRI Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI