Restriction map for
pF1KSDA1399
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Alw44I 558 GTGCAC G^TGCAC
AocI 1103 CCTCAGG CC^T[ACGT]AGG
ApaLI 558 GTGCAC G^TGCAC
Asp700I 147 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 558 GTGCAC G[AT]GC[AT]^C
AvaI 278 CCCGAG C^[CT]CG[GA]G
BbsI 426 GAAGAC GAAGAC(2/6)
BglII 821 AGATCT A^GATCT
BpuAI 426 GAAGAC GAAGAC(2/6)
BsgI 1144 CTGCAC GTGCAG(16/14)
BsiHKAI 558 GTGCAC G[AT]GC[AT]^C
BsoBI 278 CCCGAG C^[CT]CG[GA]G
BspMI 378 ACCTGC ACCTGC(4/8)
BsrBI 595 GAGCGG CCGCTC(-3/-3)
BsrFI 1093 GCCGGC [GA]^CCGG[CT]
BstYI 821 AGATCT [GA]^GATC[CT]
Bsu36I 1103 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1093 GCCGGC [GA]^CCGG[CT]
DraI 904 TTTAAA TTT^AAA
Eco81I 1103 CCTCAGG CC^T[ACGT]AGG
FauI 597 GCGGG CCCGC(4/6)
HphI 1122 TCACC GGTGA(8/7)
MflI 821 AGATCT [GA]^GATC[CT]
MseI 905 TTAA T^TAA
NaeI 1093 GCCGGC GCC^GGC
NgoMIV 1093 GCCGGC G^CCGGC
NspI 128 GCATGC [GA]CATG^[CT]
PstI 667 CTGCAG CTGCA^G
SapI 132 GCTCTTC GCTCTTC(1/4)
SfcI 667 CTGCAG C^T[GA][CT]AG
SphI 128 GCATGC GCATG^C
Tru9I 905 TTAA T^TAA
XhoII 821 AGATCT [GA]^GATC[CT]
XmnI 147 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 164 GGCGCC G[GA]^CG[CT]C
247 GGCGCC
ApaI 273 GGGCCC GGGCC^C
1077 GGGCCC
BbeI 164 GGCGCC GGCGC^C
247 GGCGCC
BlpI 390 GCTCAGC GC^T[ACGT]AGC
525 GCTGAGC
Bpu1102I 390 GCTCAGC GC^T[ACGT]AGC
525 GCTGAGC
BsaHI 164 GGCGCC G[GA]^CG[CT]C
247 GGCGCC
BsmFI 1116 GTCCC GGGAC(10/14)
1184 GTCCC
BspHI 1170 TCATGA T^CATGA
1211 TCATGA
CelII 390 GCTCAGC GC^T[ACGT]AGC
525 GCTGAGC
EaeI 221 CGGCCA [CT]^GGCC[GA]
1095 CGGCCA
Hin1I 164 GGCGCC G[GA]^CG[CT]C
247 GGCGCC
HincII 771 GTCAAC GT[CT]^[GA]AC
922 GTCAAC
HindII 771 GTCAAC GT[CT]^[GA]AC
922 GTCAAC
HinfI 1155 GAGTC G^A[ACGT]TC
1217 GAGTC
KasI 164 GGCGCC G^GCGCC
247 GGCGCC
MaeII 638 ACGT A^CGT
851 ACGT
MaeIII 1157 GTCAC ^GT[ACGT]AC
1219 GTCAC
MspA1I 120 CCGCTG C[AC]G^C[GT]G
537 CCGCTG
NarI 164 GGCGCC GG^CGCC
247 GGCGCC
PleI 1155 GAGTC GAGTC(4/5)
1217 GAGTC
PpuMI 475 AGGACCC [GA]G^G[AT]CC[CT]
882 AGGACCC
RcaI 1170 TCATGA T^CATGA
1211 TCATGA
SfoI 164 GGCGCC GGC^GCC
247 GGCGCC
Tsp45I 1157 GTCAC ^GT[GC]AC
1219 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
Ksp632I MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TseI TspRI