Restriction map for
pF1KSDA1260
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbrPI
BbvCI BcgI BfrI BlnI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoT22I
FseI FspI HindIII KpnI KspI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 2415 AAATTT [GA]^AATT[CT]
AgeI 637 ACCGGT A^CCGGT
ApoI 2415 AAATTT [GA]^AATT[CT]
Asp700I 1774 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 756 CTCGGG C^[CT]CG[GA]G
BglI 2038 GCCGTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 194 AGATCT A^GATCT
BlpI 1554 GCTCAGC GC^T[ACGT]AGC
BpmI 248 CTGGAG CTGGAG(16/14)
Bpu1102I 1554 GCTCAGC GC^T[ACGT]AGC
BsaAI 454 TACGTG [CT]AC^GT[GA]
BsaBI 1603 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 811 GGTCTC GGTCTC(1/5)
BsaWI 637 ACCGGT [AT]^CCGG[AT]
BsgI 1375 CTGCAC GTGCAG(16/14)
BsiEI 2296 CGGTCG CG[GA][CT]^CG
BsmBI 1300 GAGACG CGTCTC(1/5)
BsmI 946 GAATGC GAATGC(1/-1)
BsoBI 756 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1268 TGTACA T^GTACA
BsrBI 837 GAGCGG CCGCTC(-3/-3)
BsrDI 2034 CATTGC GCAATG(2/0)
BsrGI 1268 TGTACA T^GTACA
BssSI 2221 CACGAG CACGAG(-5/-1)
BstEII 640 GGTGACC G^GT[ACGT]ACC
BstPI 640 GGTGACC G^GT[ACGT]ACC
CelII 1554 GCTCAGC GC^T[ACGT]AGC
EcoO65I 640 GGTGACC G^GT[ACGT]ACC
EcoRV 2158 GATATC GAT^ATC
HpaI 84 GTTAAC GTT^AAC
MamI 1603 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 876 GCCGGC GCC^GGC
NgoMIV 876 GCCGGC G^CCGGC
PinAI 637 ACCGGT A^CCGGT
SapI 1518 GCTCTTC GCTCTTC(1/4)
ScaI 215 AGTACT AGT^ACT
SspBI 1268 TGTACA T^GTACA
StuI 1125 AGGCCT AGG^CCT
TaqI 1031 TCGA T^CGA
TthHB8I 1031 TCGA T^CGA
XcmI 188 CCAATGAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1774 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
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AatII 1039 GACGTC GACGT^C
1546 GACGTC
AccII 1304 CGCG CG^CG
1380 CGCG
AocI 1621 CCTCAGG CC^T[ACGT]AGG
1972 CCTGAGG
AspHI 973 GAGCTC G[AT]GC[AT]^C
1516 GAGCTC
AspI 789 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
904 GACAAGGTC
BalI 1361 TGGCCA TGG^CCA
1897 TGGCCA
BbeI 2108 GGCGCC GGCGC^C
2126 GGCGCC
BbsI 430 GAAGAC GAAGAC(2/6)
1486 GTCTTC
BclI 480 TGATCA T^GATCA
593 TGATCA
BfaI 622 CTAG C^TAG
2441 CTAG
BpuAI 430 GAAGAC GAAGAC(2/6)
1486 GTCTTC
BseRI 2053 CTCCTC GAGGAG(10/8)
703 GAGGAG
BsiHKAI 973 GAGCTC G[AT]GC[AT]^C
1516 GAGCTC
BspHI 477 TCATGA T^CATGA
1568 TCATGA
BspMI 1373 ACCTGC ACCTGC(4/8)
2263 ACCTGC
BstAPI 360 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2367 GCAGCCTTTGC
BstUI 1304 CGCG CG^CG
1380 CGCG
BstXI 932 CCACGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1199 CCAACTTCGTGG
Bsu36I 1621 CCTCAGG CC^T[ACGT]AGG
1972 CCTGAGG
DrdI 1033 GACGGCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1978 GACACAACTGTC
Eco81I 1621 CCTCAGG CC^T[ACGT]AGG
1972 CCTGAGG
FbaI 480 TGATCA T^GATCA
593 TGATCA
KasI 2108 GGCGCC G^GCGCC
2126 GGCGCC
MaeI 622 CTAG C^TAG
2441 CTAG
MluNI 1361 TGGCCA TGG^CCA
1897 TGGCCA
MscI 1361 TGGCCA TGG^CCA
1897 TGGCCA
MvnI 1304 CGCG CG^CG
1380 CGCG
NarI 2108 GGCGCC GG^CGCC
2126 GGCGCC
NcoI 516 CCATGG C^CATGG
1464 CCATGG
NspI 365 ACATGC [GA]CATG^[CT]
920 ACATGC
PflFI 789 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
904 GACAAGGTC
PleI 2118 GACTC GAGTC(4/5)
2230 GAGTC
PstI 2194 CTGCAG CTGCA^G
2236 CTGCAG
RcaI 477 TCATGA T^CATGA
1568 TCATGA
SacI 973 GAGCTC GAGCT^C
1516 GAGCTC
SfcI 2194 CTGCAG C^T[GA][CT]AG
2236 CTGCAG
SfoI 2108 GGCGCC GGC^GCC
2126 GGCGCC
Tth111I 789 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
904 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbvI
BciVI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII