Restriction map for
pF1KSDA1152
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BcnI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HpaI KasI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 905 GGTACC G^GTACC
AcsI 856 AAATTT [GA]^AATT[CT]
AflIII 650 ACATGT A^C[GA][CT]GT
ApoI 856 AAATTT [GA]^AATT[CT]
Asp700I 836 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 905 GGTACC G^GTACC
AvaII 530 GGACC G^G[AT]CC
BanI 905 GGTACC G^G[CT][GA]CC
BbvCI 26 CCTCAGC CCTCAGC(-5/-2)
BciVI 1045 GTATCC GTATCC(6/5)
BclI 813 TGATCA T^GATCA
BfaI 422 CTAG C^TAG
BlpI 87 GCTGAGC GC^T[ACGT]AGC
BpmI 932 CTGGAG CTGGAG(16/14)
Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC
BsaWI 240 TCCGGT [AT]^CCGG[AT]
BseRI 76 GAGGAG GAGGAG(10/8)
BspLU11I 650 ACATGT A^CATGT
BsrI 694 ACTGG ACTGG(1/-1)
BstEII 243 GGTGACC G^GT[ACGT]ACC
BstPI 243 GGTGACC G^GT[ACGT]ACC
CelII 87 GCTGAGC GC^T[ACGT]AGC
Cfr13I 530 GGACC G^G[ACGT]CC
DpnII 814 GATC ^GATC
DsaI 543 CCACGG C^C[GA][CT]GG
EaeI 889 CGGCCA [CT]^GGCC[GA]
EcoNI 951 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 243 GGTGACC G^GT[ACGT]ACC
EcoT14I 32 CCTTGG C^C[AT][AT]GG
FauI 506 CCCGC CCCGC(4/6)
FbaI 813 TGATCA T^GATCA
HaeII 84 GGCGCT [GA]GCGC^[CT]
HapII 241 CCGG C^CGG
HincII 368 GTCGAC GT[CT]^[GA]AC
HindII 368 GTCGAC GT[CT]^[GA]AC
HindIII 58 AAGCTT A^AGCTT
HpaII 241 CCGG C^CGG
KpnI 905 GGTACC GGTAC^C
MaeI 422 CTAG C^TAG
MboI 814 GATC ^GATC
MspA1I 507 CCGCTG C[AC]G^C[GT]G
MspI 241 CCGG C^CGG
NdeII 814 GATC ^GATC
NheI 421 GCTAGC G^CTAGC
NspI 650 ACATGT [GA]CATG^[CT]
SalI 368 GTCGAC G^TCGAC
Sau3AI 814 GATC ^GATC
Sau96I 530 GGACC G^G[ACGT]CC
SfaNI 947 GATGC GCATC(5/9)
SspI 1024 AATATT AAT^ATT
StuI 188 AGGCCT AGG^CCT
StyI 32 CCTTGG C^C[AT][AT]GG
XmnI 836 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 368 GTCGAC GT^[AC][GT]AC
926 GTCTAC
AfaI 13 GTAC GT^AC
906 GTAC
AlwNI 433 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
496 CAGCGTCTG
BsiYI 507 CCGCTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
951 CCTCAGAAAGG
BslI 507 CCGCTGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
951 CCTCAGAAAGG
BspMI 172 ACCTGC ACCTGC(4/8)
473 GCAGGT
CfoI 85 GCGC GCG^C
381 GCGC
HgaI 138 GCGTC GACGC(5/10)
498 GCGTC
HhaI 85 GCGC GCG^C
381 GCGC
HinP1I 85 GCGC G^CGC
381 GCGC
MboII 965 GAAGA GAAGA(8/7)
792 TCTTC
NlaIII 334 CATG CATG^
651 CATG
PstI 471 CTGCAG CTGCA^G
938 CTGCAG
RsaI 13 GTAC GT^AC
906 GTAC
Tsp509I 250 AATT ^AATT
857 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I DdeI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MnlI MvaI MwoI NlaIV
PleI ScrFI SfcI TaqI TfiI
TseI Tsp45I TspRI TthHB8I