Restriction map for
pF1KSDA1125
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoRI FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 3324 GACGTC GACGT^C
AcsI 980 AAATTC [GA]^AATT[CT]
AflIII 3481 ACACGT A^C[GA][CT]GT
AgeI 2280 ACCGGT A^CCGGT
Alw44I 1318 GTGCAC G^TGCAC
ApaLI 1318 GTGCAC G^TGCAC
ApoI 980 AAATTC [GA]^AATT[CT]
Asp700I 258 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 185 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 90 TGGCCA TGG^CCA
BanI 2320 GGCGCC G^G[CT][GA]CC
BbeI 2320 GGCGCC GGCGC^C
BglI 634 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1593 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1593 GCTGAGC GC^T[ACGT]AGC
BsaAI 1045 TACGTG [CT]AC^GT[GA]
BsaWI 2280 ACCGGT [AT]^CCGG[AT]
Bsp1407I 586 TGTACA T^GTACA
BsrGI 586 TGTACA T^GTACA
BstAPI 2502 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1593 GCTGAGC GC^T[ACGT]AGC
DraII 937 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 898 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2985 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 937 AGGGCCT [GA]G^G[ACGT]CC[CT]
HincII 2725 GTCAAC GT[CT]^[GA]AC
HindII 2725 GTCAAC GT[CT]^[GA]AC
KasI 2320 GGCGCC G^GCGCC
KspI 2312 CCGCGG CCGC^GG
MluNI 90 TGGCCA TGG^CCA
MscI 90 TGGCCA TGG^CCA
NaeI 1156 GCCGGC GCC^GGC
NarI 2320 GGCGCC GG^CGCC
NgoMIV 1156 GCCGGC G^CCGGC
NspI 2498 ACATGC [GA]CATG^[CT]
PaeR7I 3270 CTCGAG C^TCGAG
PflFI 185 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 570 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2280 ACCGGT A^CCGGT
PstI 35 CTGCAG CTGCA^G
SacII 2312 CCGCGG CCGC^GG
SfcI 35 CTGCAG C^T[GA][CT]AG
SfoI 2320 GGCGCC GGC^GCC
SgrAI 2279 CACCGGTG C[GA]^CCGG[CT]G
SmaI 320 CCCGGG CCC^GGG
SspBI 586 TGTACA T^GTACA
Tth111I 185 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 570 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3270 CTCGAG C^TCGAG
XmaCI 320 CCCGGG C^CCGGG
XmaI 320 CCCGGG C^CCGGG
XmnI 258 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 1289 CGCG CG^CG
2313 CGCG
BfaI 778 CTAG C^TAG
2789 CTAG
BsaI 418 GAGACC GGTCTC(1/5)
3061 GAGACC
BsiEI 1745 CGGCCG CG[GA][CT]^CG
2435 CGGCCG
BsrBI 2292 CCGCTC CCGCTC(-3/-3)
2995 GAGCGG
BstUI 1289 CGCG CG^CG
2313 CGCG
BstXI 2246 CCACGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2648 CCATCACCCTGG
BstYI 24 AGATCC [GA]^GATC[CT]
1223 AGATCC
EagI 1745 CGGCCG C^GGCCG
2435 CGGCCG
EclXI 1745 CGGCCG C^GGCCG
2435 CGGCCG
Eco52I 1745 CGGCCG C^GGCCG
2435 CGGCCG
EcoRV 4 GATATC GAT^ATC
2579 GATATC
EcoT22I 411 ATGCAT ATGCA^T
512 ATGCAT
MaeI 778 CTAG C^TAG
2789 CTAG
MflI 24 AGATCC [GA]^GATC[CT]
1223 AGATCC
MvnI 1289 CGCG CG^CG
2313 CGCG
NcoI 1394 CCATGG C^CATGG
2963 CCATGG
NsiI 411 ATGCAT ATGCA^T
512 ATGCAT
SapI 3513 GAAGAGC GCTCTTC(1/4)
2294 GCTCTTC
SmlI 472 CTCAAG C^T[CT][GA]AG
3270 CTCGAG
XhoII 24 AGATCC [GA]^GATC[CT]
1223 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI