Restriction map for
pF1KSDA1051
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AvaI AvaII AviII BamHI
BbrPI BbvCI BfrI BglI BglII
BsaAI BsaBI BsaI BseAI BseRI
BsgI BsiEI BsiWI BsmI BsoBI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FseI FspI HgaI HindIII HpaI
KspI MamI MflI MluI MroI
MseI MspA1I NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 511 GTCTAC GT^[AC][GT]AC
AccII 876 CGCG CG^CG
AcyI 158 GGCGCC G[GA]^CG[CT]C
AflIII 582 ACATGT A^C[GA][CT]GT
AlwI 304 GATCC GGATC(4/5)
ApaI 174 GGGCCC GGGCC^C
AspI 249 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 276 CCTAGG C^CTAGG
BalI 130 TGGCCA TGG^CCA
BbeI 158 GGCGCC GGCGC^C
BbsI 799 GAAGAC GAAGAC(2/6)
BbvI 613 GCAGC GCAGC(8/12)
BcnI 19 CCGGG CC^[GC]GG
BfaI 277 CTAG C^TAG
BlnI 276 CCTAGG C^CTAGG
BlpI 291 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 291 GCTGAGC GC^T[ACGT]AGC
BpuAI 799 GAAGAC GAAGAC(2/6)
BsaHI 158 GGCGCC G[GA]^CG[CT]C
BsaWI 993 TCCGGT [AT]^CCGG[AT]
BsmBI 923 GAGACG CGTCTC(1/5)
BsmFI 918 GGGAC GGGAC(10/14)
Bsp1407I 488 TGTACA T^GTACA
BspDI 52 ATCGAT AT^CGAT
BspLU11I 582 ACATGT A^CATGT
BsrGI 488 TGTACA T^GTACA
BstF5I 371 GGATG GGATG(2/0)
BstUI 876 CGCG CG^CG
CelII 291 GCTGAGC GC^T[ACGT]AGC
CfoI 159 GCGC GCG^C
ClaI 52 ATCGAT AT^CGAT
EaeI 130 TGGCCA [CT]^GGCC[GA]
EcoT14I 276 CCTAGG C^C[AT][AT]GG
FauI 1008 CCCGC CCCGC(4/6)
FokI 371 GGATG GGATG(9/13)
HaeII 158 GGCGCC [GA]GCGC^[CT]
HhaI 159 GCGC GCG^C
Hin1I 158 GGCGCC G[GA]^CG[CT]C
HinP1I 159 GCGC G^CGC
KasI 158 GGCGCC G^GCGCC
MaeI 277 CTAG C^TAG
MaeIII 247 GTGAC ^GT[ACGT]AC
MfeI 149 CAATTG C^AATTG
MluNI 130 TGGCCA TGG^CCA
MscI 130 TGGCCA TGG^CCA
MunI 149 CAATTG C^AATTG
MvnI 876 CGCG CG^CG
NarI 158 GGCGCC GG^CGCC
NciI 19 CCGGG CC^[GC]GG
NdeI 869 CATATG CA^TATG
NspI 582 ACATGT [GA]CATG^[CT]
PflFI 249 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 433 CCAGTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 249 GACTC GAGTC(4/5)
SacI 740 GAGCTC GAGCT^C
SapI 616 GCTCTTC GCTCTTC(1/4)
SfaNI 169 GCATC GCATC(5/9)
SfoI 158 GGCGCC GGC^GCC
SspBI 488 TGTACA T^GTACA
StyI 276 CCTAGG C^C[AT][AT]GG
TseI 613 GCAGC G^C[AT]GC
Tsp45I 247 GTGAC ^GT[GC]AC
Tth111I 249 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 433 CCAGTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 615 AGCT AG^CT
741 AGCT
BcgI 54 CGATTCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
87 CGAATATGTTGC
BclI 81 TGATCA T^GATCA
959 TGATCA
BsaJI 213 CCTGGG C^C[ACGT][ACGT]GG
276 CCTAGG
BsiYI 433 CCAGTAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
987 CCAGCCTCCGG
BslI 433 CCAGTAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
987 CCAGCCTCCGG
BsmAI 923 GAGAC GTCTC(1/5)
255 GTCTC
Cac8I 287 GCTGGC GC[ACGT]^[ACGT]GC
986 GCCAGC
Cfr13I 174 GGGCC G^G[ACGT]CC
175 GGCCC
EarI 617 CTCTTC CTCTTC(1/4)
702 GAAGAG
EcoRI 107 GAATTC G^AATTC
829 GAATTC
FbaI 81 TGATCA T^GATCA
959 TGATCA
HaeIII 131 GGCC GG^CC
175 GGCC
HincII 208 GTTGAC GT[CT]^[GA]AC
532 GTTGAC
HindII 208 GTTGAC GT[CT]^[GA]AC
532 GTTGAC
Ksp632I 617 CTCTTC CTCTTC(1/4)
702 GAAGAG
MaeII 509 ACGT A^CGT
820 ACGT
MslI 85 CACGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
543 CATAGAAATG
Sau96I 174 GGGCC G^G[ACGT]CC
175 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
Acc65I AciI AcsI AfaI ApoI
Asp718I AspHI BanI BanII BciVI
BmyI BpmI BsiHKAI Bsp1286I BsrI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI HapII HinfI HpaII HphI
ItaI KpnI MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI TaqI
TfiI Tsp509I TspRI TthHB8I