Restriction map for
pF1KSDA0980
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII AvrII BamHI BcgI BciVI
BlnI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I EcoO65I EcoRV
EcoT22I FspI MamI MfeI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3199 GACGTC GACGT^C
Acc65I 1325 GGTACC G^GTACC
AccI 43 GTCTAC GT^[AC][GT]AC
AflII 109 CTTAAG C^TTAAG
Aor51HI 3147 AGCGCT AGC^GCT
Asp700I 4087 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1325 GGTACC G^GTACC
AspI 3199 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2528 CACGTG CAC^GTG
BclI 972 TGATCA T^GATCA
BfaI 811 CTAG C^TAG
BfrI 109 CTTAAG C^TTAAG
BglII 1022 AGATCT A^GATCT
BsiEI 2891 CGGCCG CG[GA][CT]^CG
BsmI 3616 GAATGC GAATGC(1/-1)
BspLU11I 3222 ACATGT A^CATGT
BssHII 2382 GCGCGC G^CGCGC
DraI 3369 TTTAAA TTT^AAA
DrdI 1111 GACAGTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2891 CGGCCG C^GGCCG
EclXI 2891 CGGCCG C^GGCCG
Eco47III 3147 AGCGCT AGC^GCT
Eco52I 2891 CGGCCG C^GGCCG
EcoRI 769 GAATTC G^AATTC
FbaI 972 TGATCA T^GATCA
FseI 2888 GGCCGGCC GGCCGG^CC
KpnI 1325 GGTACC GGTAC^C
MaeI 811 CTAG C^TAG
MluI 375 ACGCGT A^CGCGT
NcoI 3652 CCATGG C^CATGG
PflFI 3199 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2587 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2528 CACGTG CAC^GTG
PmlI 2528 CACGTG CAC^GTG
ScaI 3334 AGTACT AGT^ACT
SfiI 2574 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2723 GCATGC GCATG^C
Tth111I 3199 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2587 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 810 TCTAGA T^CTAGA
XmnI 4087 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 193 GAATTT [GA]^AATT[CT]
769 GAATTC
ApaI 1082 GGGCCC GGGCC^C
2455 GGGCCC
ApoI 193 GAATTT [GA]^AATT[CT]
769 GAATTC
BalI 983 TGGCCA TGG^CCA
2514 TGGCCA
BsaAI 2528 CACGTG [CT]AC^GT[GA]
4000 TACGTG
BspHI 792 TCATGA T^CATGA
1100 TCATGA
BssSI 2047 CACGAG CACGAG(-5/-1)
904 CTCGTG
BstXI 2569 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2863 CCACGGATGTGG
BstYI 599 AGATCC [GA]^GATC[CT]
1022 AGATCT
HincII 181 GTTAAC GT[CT]^[GA]AC
3537 GTTAAC
HindII 181 GTTAAC GT[CT]^[GA]AC
3537 GTTAAC
HindIII 112 AAGCTT A^AGCTT
1528 AAGCTT
HpaI 181 GTTAAC GTT^AAC
3537 GTTAAC
KspI 2103 CCGCGG CCGC^GG
2436 CCGCGG
MflI 599 AGATCC [GA]^GATC[CT]
1022 AGATCT
MluNI 983 TGGCCA TGG^CCA
2514 TGGCCA
MscI 983 TGGCCA TGG^CCA
2514 TGGCCA
NaeI 338 GCCGGC GCC^GGC
2889 GCCGGC
NgoMIV 338 GCCGGC G^CCGGC
2889 GCCGGC
NspI 2723 GCATGC [GA]CATG^[CT]
3222 ACATGT
PaeR7I 697 CTCGAG C^TCGAG
2410 CTCGAG
RcaI 792 TCATGA T^CATGA
1100 TCATGA
SacII 2103 CCGCGG CCGC^GG
2436 CCGCGG
SmaI 2813 CCCGGG CCC^GGG
2996 CCCGGG
XhoI 697 CTCGAG C^TCGAG
2410 CTCGAG
XhoII 599 AGATCC [GA]^GATC[CT]
1022 AGATCT
XmaCI 2813 CCCGGG C^CCGGG
2996 CCCGGG
XmaI 2813 CCCGGG C^CCGGG
2996 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I SbfI ScrFI SexAI
SfaNI SfcI SfoI SmlI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI