Restriction map for
pF1KSDA0952
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflII 1282 CTTAAG C^TTAAG
AlwNI 627 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1118 AGCGCT AGC^GCT
Asp700I 808 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 581 TGGCCA TGG^CCA
BanII 435 GAGCTC G[GA]GC[CT]^C
BbsI 165 GAAGAC GAAGAC(2/6)
BfrI 1282 CTTAAG C^TTAAG
BglII 900 AGATCT A^GATCT
BpuAI 165 GAAGAC GAAGAC(2/6)
BsmBI 58 GAGACG CGTCTC(1/5)
Bsp1407I 363 TGTACA T^GTACA
BspMI 510 ACCTGC ACCTGC(4/8)
BsrGI 363 TGTACA T^GTACA
BstAPI 991 GCAAATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 1103 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 383 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 581 TGGCCA [CT]^GGCC[GA]
Eco47III 1118 AGCGCT AGC^GCT
EcoNI 699 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1103 AGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 236 GCGTC GACGC(5/10)
MluNI 581 TGGCCA TGG^CCA
MscI 581 TGGCCA TGG^CCA
MslI 412 CACAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1245 CAGCTG C[AC]G^C[GT]G
PflMI 382 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 393 GACTC GAGTC(4/5)
PvuII 1245 CAGCTG CAG^CTG
SacI 435 GAGCTC GAGCT^C
ScaI 1322 AGTACT AGT^ACT
SexAI 384 ACCAGGT A^CC[AT]GGT
SspBI 363 TGTACA T^GTACA
Van91I 382 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 808 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 494 GTATAC GT^[AC][GT]AC
1053 GTATAC
AciI 960 CCGC CCGC(-3/-1)
1287 GCGG
AlwI 957 GATCC GGATC(4/5)
1542 GATCC
AvaII 74 GGTCC G^G[AT]CC
1337 GGTCC
BcgI 507 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
981 CGATGATTTTGC
BcnI 282 CCCGG CC^[GC]GG
1010 CCGGG
BfaI 805 CTAG C^TAG
937 CTAG
BsaI 649 GAGACC GGTCTC(1/5)
1030 GAGACC
BsgI 1001 CTGCAC GTGCAG(16/14)
1375 GTGCAG
BsrDI 1218 CATTGC GCAATG(2/0)
1424 GCAATG
Bst1107I 494 GTATAC GTA^TAC
1053 GTATAC
BstF5I 1188 CATCC GGATG(2/0)
1419 GGATG
BstYI 900 AGATCT [GA]^GATC[CT]
1541 AGATCC
BstZ17I 494 GTATAC GTA^TAC
1053 GTATAC
CfoI 1119 GCGC GCG^C
1164 GCGC
FokI 1188 CATCC GGATG(9/13)
1419 GGATG
HaeII 1118 AGCGCT [GA]GCGC^[CT]
1163 GGCGCT
HapII 283 CCGG C^CGG
1010 CCGG
HhaI 1119 GCGC GCG^C
1164 GCGC
HinP1I 1119 GCGC G^CGC
1164 GCGC
HpaII 283 CCGG C^CGG
1010 CCGG
MaeI 805 CTAG C^TAG
937 CTAG
MflI 900 AGATCT [GA]^GATC[CT]
1541 AGATCC
MspI 283 CCGG C^CGG
1010 CCGG
NciI 282 CCCGG CC^[GC]GG
1010 CCGGG
PstI 1058 CTGCAG CTGCA^G
1256 CTGCAG
SfcI 1058 CTGCAG C^T[GA][CT]AG
1256 CTGCAG
TfiI 258 GAATC G^A[AT]TC
779 GATTC
XcmI 839 CCAAAGAAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1406 CCAGTGTGATACTGG
XhoII 900 AGATCT [GA]^GATC[CT]
1541 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AfaI AluI AspHI BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I