Restriction map for
pF1KSDA0939
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AccII AccIII AclI
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BciVI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1462 GACGTC GACGT^C
AflII 1570 CTTAAG C^TTAAG
AlwNI 1732 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 816 AGCGCT AGC^GCT
ApaI 1704 GGGCCC GGGCC^C
BanI 1345 GGCACC G^G[CT][GA]CC
BbvCI 1470 CCTCAGC CCTCAGC(-5/-2)
BcgI 92 CGAAACTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 905 TGATCA T^GATCA
BcnI 1221 CCGGG CC^[GC]GG
BfaI 205 CTAG C^TAG
BfrI 1570 CTTAAG C^TTAAG
BpmI 1315 CTGGAG CTGGAG(16/14)
BsaBI 1248 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 694 GTCTC GTCTC(1/5)
BspHI 45 TCATGA T^CATGA
BspMI 1640 ACCTGC ACCTGC(4/8)
BsrBI 1178 GAGCGG CCGCTC(-3/-3)
BstAPI 1731 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1031 AGATCC [GA]^GATC[CT]
DraI 325 TTTAAA TTT^AAA
Eco47III 816 AGCGCT AGC^GCT
FauI 1275 GCGGG CCCGC(4/6)
FbaI 905 TGATCA T^GATCA
HaeII 816 AGCGCT [GA]GCGC^[CT]
HincII 1465 GTCAAC GT[CT]^[GA]AC
HindII 1465 GTCAAC GT[CT]^[GA]AC
MaeI 205 CTAG C^TAG
MaeII 1463 ACGT A^CGT
MamI 1248 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1031 AGATCC [GA]^GATC[CT]
NciI 1221 CCGGG CC^[GC]GG
NcoI 1322 CCATGG C^CATGG
PflMI 1676 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 812 CTGCAG CTGCA^G
PvuII 716 CAGCTG CAG^CTG
RcaI 45 TCATGA T^CATGA
SacI 1522 GAGCTC GAGCT^C
SapI 1368 GCTCTTC GCTCTTC(1/4)
SfcI 812 CTGCAG C^T[GA][CT]AG
SspI 448 AATATT AAT^ATT
TfiI 944 GAATC G^A[AT]TC
Van91I 1676 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1376 CCATCCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1031 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflIII 371 ACATGT A^C[GA][CT]GT
1078 ACATGT
BalI 608 TGGCCA TGG^CCA
965 TGGCCA
BglI 924 GCCTTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1706 GCCCCTCCGGC
BsgI 1642 CTGCAC GTGCAG(16/14)
133 GTGCAG
BsmFI 481 GGGAC GGGAC(10/14)
993 GTCCC
BspLU11I 371 ACATGT A^CATGT
1078 ACATGT
CfoI 817 GCGC GCG^C
1653 GCGC
EaeI 608 TGGCCA [CT]^GGCC[GA]
965 TGGCCA
EarI 1369 CTCTTC CTCTTC(1/4)
19 GAAGAG
HapII 1221 CCGG C^CGG
1712 CCGG
HhaI 817 GCGC GCG^C
1653 GCGC
HinP1I 817 GCGC G^CGC
1653 GCGC
HpaII 1221 CCGG C^CGG
1712 CCGG
Ksp632I 1369 CTCTTC CTCTTC(1/4)
19 GAAGAG
MluNI 608 TGGCCA TGG^CCA
965 TGGCCA
MscI 608 TGGCCA TGG^CCA
965 TGGCCA
MspA1I 171 CAGCGG C[AC]G^C[GT]G
716 CAGCTG
MspI 1221 CCGG C^CGG
1712 CCGG
PleI 409 GAGTC GAGTC(4/5)
1504 GAGTC
PpuMI 1565 AGGACCT [GA]G^G[AT]CC[CT]
1637 AGGACCT
SmlI 870 CTTGAG C^T[CT][GA]AG
1570 CTTAAG
TaqI 599 TCGA T^CGA
1427 TCGA
TthHB8I 599 TCGA T^CGA
1427 TCGA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbvI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI Hin1I HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI