Restriction map for
pF1KSDA0927
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# NO CUT ENZYMES ( 87 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbsI
BclI BfrI BlnI BpuAI BsaBI
BsiWI BspDI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoO65I EcoRI FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MunI NarI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 2622 GACGTC GACGT^C
AccIII 1112 TCCGGA T^CCGGA
AcyI 2622 GACGTC G[GA]^CG[CT]C
Aor51HI 81 AGCGCT AGC^GCT
BalI 1043 TGGCCA TGG^CCA
BbrPI 1975 CACGTG CAC^GTG
BciVI 2452 GGATAC GTATCC(6/5)
BglII 462 AGATCT A^GATCT
BsaHI 2622 GACGTC G[GA]^CG[CT]C
BsaI 778 GAGACC GGTCTC(1/5)
BsaWI 1112 TCCGGA [AT]^CCGG[AT]
BseAI 1112 TCCGGA T^CCGGA
BsmBI 2620 GAGACG CGTCTC(1/5)
BsmI 1822 GAATGC GAATGC(1/-1)
Bsp1407I 2465 TGTACA T^GTACA
BspEI 1112 TCCGGA T^CCGGA
BspHI 2198 TCATGA T^CATGA
BsrFI 1190 GCCGGC [GA]^CCGG[CT]
BsrGI 2465 TGTACA T^GTACA
Cfr10I 1190 GCCGGC [GA]^CCGG[CT]
DrdI 1536 GACCAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 81 AGCGCT AGC^GCT
EcoRV 2005 GATATC GAT^ATC
Hin1I 2622 GACGTC G[GA]^CG[CT]C
MluNI 1043 TGGCCA TGG^CCA
MroI 1112 TCCGGA T^CCGGA
MscI 1043 TGGCCA TGG^CCA
MseI 2137 TTAA T^TAA
NaeI 1190 GCCGGC GCC^GGC
NcoI 716 CCATGG C^CATGG
NgoMIV 1190 GCCGGC G^CCGGC
NheI 115 GCTAGC G^CTAGC
PmaCI 1975 CACGTG CAC^GTG
PmlI 1975 CACGTG CAC^GTG
PstI 840 CTGCAG CTGCA^G
RcaI 2198 TCATGA T^CATGA
SmlI 364 CTCAAG C^T[CT][GA]AG
SspBI 2465 TGTACA T^GTACA
StuI 1160 AGGCCT AGG^CCT
Tru9I 2137 TTAA T^TAA
Tsp509I 1430 AATT ^AATT
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccI 940 GTCTAC GT^[AC][GT]AC
1105 GTCTAC
Alw44I 921 GTGCAC G^TGCAC
1366 GTGCAC
AocI 143 CCTCAGG CC^T[ACGT]AGG
862 CCTGAGG
ApaI 1806 GGGCCC GGGCC^C
2581 GGGCCC
ApaLI 921 GTGCAC G^TGCAC
1366 GTGCAC
AspI 750 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACGAGGTC
BbvCI 2370 CCTCAGC CCTCAGC(-5/-2)
393 GCTGAGG
BcgI 105 CGAGGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2617 GCAGAGACGTCG
BfaI 116 CTAG C^TAG
460 CTAG
BsaAI 1942 TACGTG [CT]AC^GT[GA]
1975 CACGTG
BsiEI 13 CGGCCG CG[GA][CT]^CG
1383 CGGCCG
BstAPI 265 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
330 GCACGCCTTGC
BstXI 710 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2566 CCATCCCACTGG
Bsu36I 143 CCTCAGG CC^T[ACGT]AGG
862 CCTGAGG
EagI 13 CGGCCG C^GGCCG
1383 CGGCCG
EclXI 13 CGGCCG C^GGCCG
1383 CGGCCG
Eco52I 13 CGGCCG C^GGCCG
1383 CGGCCG
Eco81I 143 CCTCAGG CC^T[ACGT]AGG
862 CCTGAGG
EcoT22I 1296 ATGCAT ATGCA^T
1824 ATGCAT
HgaI 448 GCGTC GACGC(5/10)
1388 GCGTC
MaeI 116 CTAG C^TAG
460 CTAG
NsiI 1296 ATGCAT ATGCA^T
1824 ATGCAT
NspI 1294 ACATGC [GA]CATG^[CT]
2422 GCATGT
PflFI 750 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACGAGGTC
PflMI 1791 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2073 CCACATCTTGG
PvuII 391 CAGCTG CAG^CTG
2373 CAGCTG
SapI 97 GCTCTTC GCTCTTC(1/4)
1549 GCTCTTC
SfiI 684 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1644 GGCCCGGGCGGCC
SmaI 661 CCCGGG CCC^GGG
1646 CCCGGG
TfiI 2019 GAATC G^A[AT]TC
2303 GAATC
Tth111I 750 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACGAGGTC
Van91I 1791 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2073 CCACATCTTGG
XcmI 716 CCATGGCCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1007 CCATCCGCGGGGTGG
XmaCI 661 CCCGGG C^CCGGG
1646 CCCGGG
XmaI 661 CCCGGG C^CCGGG
1646 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII