Restriction map for
pF1KSDA0915
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BbrPI BfrI BlnI
BsaAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoRV EcoT22I FseI
HgaI HincII HindII HpaI KpnI
KspI MfeI MluI MluNI MscI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI StuI SwaI Tru9I XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 1278 GACGTC GACGT^C
AccII 1964 CGCG CG^CG
AccIII 2094 TCCGGA T^CCGGA
AcsI 1906 GAATTC [GA]^AATT[CT]
AfaI 1056 GTAC GT^AC
Alw44I 171 GTGCAC G^TGCAC
ApaLI 171 GTGCAC G^TGCAC
ApoI 1906 GAATTC [GA]^AATT[CT]
AspI 2321 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2476 CTCGAG C^[CT]CG[GA]G
AviII 1688 TGCGCA TGC^GCA
BamHI 2097 GGATCC G^GATCC
BbeI 1898 GGCGCC GGCGC^C
BbsI 1217 GTCTTC GAAGAC(2/6)
BcgI 1775 GCAAGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1224 GGATAC GTATCC(6/5)
BclI 1368 TGATCA T^GATCA
BglI 1750 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 897 AGATCT A^GATCT
BpuAI 1217 GTCTTC GAAGAC(2/6)
BsaBI 898 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2094 TCCGGA [AT]^CCGG[AT]
BseAI 2094 TCCGGA T^CCGGA
BsmI 1746 GAATGC GAATGC(1/-1)
BsoBI 2476 CTCGAG C^[CT]CG[GA]G
BspEI 2094 TCCGGA T^CCGGA
BsrFI 1607 GCCGGC [GA]^CCGG[CT]
BssSI 851 CTCGTG CACGAG(-5/-1)
BstUI 1964 CGCG CG^CG
Cfr10I 1607 GCCGGC [GA]^CCGG[CT]
DsaI 398 CCATGG C^C[GA][CT]GG
EcoRI 1906 GAATTC G^AATTC
EcoT14I 398 CCATGG C^C[AT][AT]GG
FbaI 1368 TGATCA T^GATCA
FspI 1688 TGCGCA TGC^GCA
HindIII 2174 AAGCTT A^AGCTT
KasI 1898 GGCGCC G^GCGCC
MamI 898 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2094 TCCGGA T^CCGGA
MvnI 1964 CGCG CG^CG
NaeI 1607 GCCGGC GCC^GGC
NarI 1898 GGCGCC GG^CGCC
NcoI 398 CCATGG C^CATGG
NgoMIV 1607 GCCGGC G^CCGGC
PaeR7I 2476 CTCGAG C^TCGAG
PflFI 2321 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 1056 GTAC GT^AC
SacI 664 GAGCTC GAGCT^C
SbfI 1038 CCTGCAGG CCTGCA^GG
SfoI 1898 GGCGCC GGC^GCC
Sse8387I 1038 CCTGCAGG CCTGCA^GG
StyI 398 CCATGG C^C[AT][AT]GG
TfiI 923 GAATC G^A[AT]TC
Tth111I 2321 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 241 TCTAGA T^CTAGA
XcmI 1211 CCAGCGGTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2476 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 1278 GACGTC G[GA]^CG[CT]C
1898 GGCGCC
Aor51HI 1795 AGCGCT AGC^GCT
2198 AGCGCT
BlpI 808 GCTGAGC GC^T[ACGT]AGC
1332 GCTGAGC
Bpu1102I 808 GCTGAGC GC^T[ACGT]AGC
1332 GCTGAGC
BsaHI 1278 GACGTC G[GA]^CG[CT]C
1898 GGCGCC
BstEII 374 GGTCACC G^GT[ACGT]ACC
769 GGTCACC
BstPI 374 GGTCACC G^GT[ACGT]ACC
769 GGTCACC
BstYI 897 AGATCT [GA]^GATC[CT]
2097 GGATCC
CelII 808 GCTGAGC GC^T[ACGT]AGC
1332 GCTGAGC
DraIII 1179 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1495 CACGCTGTG
Eco47III 1795 AGCGCT AGC^GCT
2198 AGCGCT
EcoO65I 374 GGTCACC G^GT[ACGT]ACC
769 GGTCACC
Hin1I 1278 GACGTC G[GA]^CG[CT]C
1898 GGCGCC
MaeII 1279 ACGT A^CGT
1583 ACGT
MflI 897 AGATCT [GA]^GATC[CT]
2097 GGATCC
MslI 1323 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1578 CACCAACGTG
PvuII 197 CAGCTG CAG^CTG
2410 CAGCTG
SapI 1825 GAAGAGC GCTCTTC(1/4)
1451 GCTCTTC
Tsp509I 1024 AATT ^AATT
1907 AATT
XhoII 897 AGATCT [GA]^GATC[CT]
2097 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI TaqI TseI
Tsp45I TspRI TthHB8I Van91I