Restriction map for
pF1KSDA0893
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
CfoI ClaI CpoI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 297 CGCG CG^CG
Alw44I 1925 GTGCAC G^TGCAC
AlwNI 322 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 153 CCTGAGG CC^T[ACGT]AGG
ApaLI 1925 GTGCAC G^TGCAC
AseI 1798 ATTAAT AT^TAAT
AsnI 1798 ATTAAT AT^TAAT
BamHI 1984 GGATCC G^GATCC
BclI 1395 TGATCA T^GATCA
BglII 2100 AGATCT A^GATCT
BsaBI 715 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2437 CGTCTC CGTCTC(1/5)
BsmFI 2618 GGGAC GGGAC(10/14)
BsmI 613 GAATGC GAATGC(1/-1)
BspHI 978 TCATGA T^CATGA
BsrBI 930 CCGCTC CCGCTC(-3/-3)
BsrFI 1947 GCCGGT [GA]^CCGG[CT]
BssSI 479 CACGAG CACGAG(-5/-1)
BstUI 297 CGCG CG^CG
Bsu36I 153 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1947 GCCGGT [GA]^CCGG[CT]
DraI 1960 TTTAAA TTT^AAA
DraIII 1673 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 153 CCTGAGG CC^T[ACGT]AGG
EcoNI 99 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2116 ATGCAT ATGCA^T
FauI 929 CCCGC CCCGC(4/6)
FbaI 1395 TGATCA T^GATCA
HapII 1948 CCGG C^CGG
HpaI 289 GTTAAC GTT^AAC
HpaII 1948 CCGG C^CGG
MamI 715 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1948 CCGG C^CGG
MvnI 297 CGCG CG^CG
NdeI 836 CATATG CA^TATG
NsiI 2116 ATGCAT ATGCA^T
PpuMI 2618 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1576 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1798 ATTAAT AT^TAAT
PstI 2720 CTGCAG CTGCA^G
RcaI 978 TCATGA T^CATGA
SacI 2558 GAGCTC GAGCT^C
SmlI 1084 CTTGAG C^T[CT][GA]AG
SpeI 1552 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanII 316 GAGCCC G[GA]GC[CT]^C
2558 GAGCTC
BsgI 2402 GTGCAG GTGCAG(16/14)
2679 GTGCAG
DraII 2237 AGGGCCT [GA]G^G[ACGT]CC[CT]
2618 GGGACCT
DrdI 968 GACTAACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2044 GACAAATACGTC
EcoO109I 2237 AGGGCCT [GA]G^G[ACGT]CC[CT]
2618 GGGACCT
EcoRI 66 GAATTC G^AATTC
520 GAATTC
EcoT14I 999 CCTTGG C^C[AT][AT]GG
1476 CCTTGG
HincII 289 GTTAAC GT[CT]^[GA]AC
805 GTTGAC
HindII 289 GTTAAC GT[CT]^[GA]AC
805 GTTGAC
MaeII 1956 ACGT A^CGT
2051 ACGT
NspI 363 ACATGC [GA]CATG^[CT]
2471 GCATGT
PflMI 321 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1717 CCACCTCTTGG
PvuII 911 CAGCTG CAG^CTG
1847 CAGCTG
StuI 600 AGGCCT AGG^CCT
1153 AGGCCT
StyI 999 CCTTGG C^C[AT][AT]GG
1476 CCTTGG
Van91I 321 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1717 CCACCTCTTGG
XbaI 210 TCTAGA T^CTAGA
369 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI